Generation of trisomy 21 induced pluripotent stem cells
21 三体诱导多能干细胞的产生
基本信息
- 批准号:7942741
- 负责人:
- 金额:$ 18.56万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-09-30 至 2012-02-29
- 项目状态:已结题
- 来源:
- 关键词:AffectAlzheimer&aposs DiseaseBiomanufacturingBrainCell LineCell modelCellsCharacteristicsChromosomesChromosomes, Human, Pair 21ClinicalCommunitiesCongenital Heart DefectsCoupledCraniofacial AbnormalitiesDataDefectDevelopmentDevelopmental ProcessDown SyndromeEmbryoEnvironmentEquipmentEvaluationFibroblastsGenerationsGeneticGoalsHousingHumanImmune systemImpairmentIndividualKnowledgeLeadLifeMental RetardationMental Retardation and Developmental Disabilities Research CentersMissionModelingMolecularNerve DegenerationNervous system structureNeurologicNeuronal DifferentiationNeuronsNeurosciencesNon-Viral VectorOrganPluripotent Stem CellsPredispositionProcessProsencephalonPsyche structurePublic HealthReagentReportingResearchResearch PersonnelResearch PriorityResourcesServicesSourceStem Cell ResearchStem cellsSynapsesSystemTestingTissuesTrisomyUniversitiesValidationViralWisconsinWorkabstractingbasecell typedevelopmental diseaseembryonic stem cellexperiencehuman diseaseinduced pluripotent stem cellinnovationinterestleukemiamouse modelneurodevelopmentneurogenesisprogramsrelating to nervous systemresearch studysuccess
项目摘要
DESCRIPTION (provided by applicant): Down Syndrome (DS), or trisomy 21, is the most common genetic developmental disorder that leads to mental retardation. Mouse models have implicated reduced neurogenesis and faulty synaptic development as important contributors to the mental impairment characteristic of DS. While mouse models are crucial, they cannot explain all the complexities of human brain development making it important to examine the effects of trisomy 21 in the context of human cells. Human neural development in DS has not been well studied. An innovative way to study the development of particular cell types is through the use of human pluripotent stem cells. This exploratory proposal aims to take advantage of recent reports of induced pluripotent stem cells (iPS cells) to create Down syndrome-specific pluripotent stem cells to facilitate analysis of human neural development. The first aim of this proposal is to induce pluripotent stem cells from human fibroblasts that carry the trisomy 21. The second aim will exploit this new resource by testing the feasibility of differentiating these trisomy 21 iPS cells into functional forebrain neurons. The establishment of this human trisomy 21 neuronal culture paradigm will enable studies dissecting the molecular mechanisms of at least three important research priorities in Down syndrome: neurogenesis, synapse development and function, and neurodegeneration. PUBLIC HEALTH RELEVANCE: As the most common genetic cause of mental retardation, Down syndrome is a public health concern. The proposed research will create a new model of Down syndrome to advance the study of Down syndrome neuropathophysiology. The aims of this proposal include making Down syndrome-specific human pluripotent stem cells from non-embryonic sources.
描述(申请人提供):唐氏综合症(DS),或21三体,是最常见的导致智力低下的遗传性发育障碍。小鼠模型表明,神经发生减少和突触发育障碍是DS的精神损害特征的重要因素。虽然老鼠模型至关重要,但它们不能解释人脑发育的所有复杂性,这使得研究21三体在人类细胞环境中的影响变得重要。人类在DS中的神经发育尚未得到很好的研究。研究特定细胞类型发育的一种创新方法是通过使用人类多能干细胞。这一探索性建议旨在利用最近关于诱导多能干细胞(iPS细胞)的报道来创造唐氏综合症特异性多能干细胞,以促进对人类神经发育的分析。这项提议的第一个目标是从携带21三体的人成纤维细胞中诱导出多能干细胞。第二个目标将通过测试将这些21三体iPS细胞分化为功能性前脑神经元的可行性来开发这一新资源。这种人类21三体神经元培养模式的建立将使研究能够剖析唐氏综合症至少三个重要研究重点的分子机制:神经发生、突触发育和功能以及神经退行性变。公共卫生相关性:唐氏综合症是导致精神发育迟滞的最常见的遗传原因,是一个公共卫生问题。本研究将建立一种新的唐氏综合征模型,促进唐氏综合征神经病理生理学研究。这项提议的目标包括从非胚胎来源中制造唐氏综合症特有的人类多能干细胞。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
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ANITA BHATTACHARYYA其他文献
ANITA BHATTACHARYYA的其他文献
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{{ truncateString('ANITA BHATTACHARYYA', 18)}}的其他基金
Supplement to TR01 Human cortical development and neural plasticity altered by trisomy 21
TR01 补充品 21 三体改变的人类皮质发育和神经可塑性
- 批准号:
10670626 - 财政年份:2022
- 资助金额:
$ 18.56万 - 项目类别:
Modeling Histone Demethylase Function in Neurogenesis
模拟神经发生中的组蛋白去甲基化酶功能
- 批准号:
10527660 - 财政年份:2022
- 资助金额:
$ 18.56万 - 项目类别:
Human cortical development and neural plasticity altered by trisomy 21
21 三体改变人类皮质发育和神经可塑性
- 批准号:
10296076 - 财政年份:2021
- 资助金额:
$ 18.56万 - 项目类别:
T21RS meeting June 2019 Barcelona meeting grant
T21RS 会议 2019 年 6 月 巴塞罗那会议补助金
- 批准号:
9763218 - 财政年份:2019
- 资助金额:
$ 18.56万 - 项目类别:
Defining interneuron deficits in Down syndrome brain.
定义唐氏综合症大脑的中间神经元缺陷。
- 批准号:
9034147 - 财政年份:2015
- 资助金额:
$ 18.56万 - 项目类别:
Generation of trisomy 21 induced pluripotent stem cells
21 三体诱导多能干细胞的产生
- 批准号:
7739054 - 财政年份:2009
- 资助金额:
$ 18.56万 - 项目类别: