A Novel Treatment for Keratoconus and Keratectasias using Nitro Technology

使用硝基技术治疗圆锥角膜和角膜扩张症的新方法

基本信息

  • 批准号:
    7754376
  • 负责人:
  • 金额:
    $ 19.8万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2009
  • 资助国家:
    美国
  • 起止时间:
    2009-01-01 至 2011-06-30
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): A major breakthrough in the treatment of keratoconus and post-LASIK keratectasias has been realized. Recent work by the German group of Wollensak, Spoerl, and Seiler has shown that cross-linking corneal collagen through application of riboflavin and ultraviolet light (UVR) can limit progressive vision loss in keratoconus patients. Despite these successes, the UVR therapy poses attendant risks, particularly related to ultraviolet irradiation. As such, FDA approval for clinical trials in the US has only recently been granted. The thrust of this application is to develop an alternative method of corneal collagen cross-linking using novel technology. Our recent studies indicate that collagen cross-linking through reactions with nitrite and related agents can induce corneal changes commensurate with UVR therapy. In addition, published toxicity/mutagenicity studies suggest a good safety profile for these agents. Thus, nitro technology could be used as an "eye drop alternative" to UVR treatment. Potential advantages over current UVR therapy include the omission of ultraviolet light exposure, the ability to treat thin corneas, less patient discomfort, ease of self- administration, a dose-response effect, and the ability to treat the peripheral cornea. This project is designed to lay groundwork for rapid translation of this technology into a treatment. Specifically, we will: 1. Determine an effective way to cross-link the cornea using nitro technology under conditions simulating the human cornea (i.e. pH 7.4 and 34oC). Fresh rabbit and human eye bank corneas will be incubated under a variety of conditions that include 1. nitrite with catalysts and 2. alternative nitrite related agents. The assay of thermal shrinkage temperature will be used to determine cross-linking efficacy. Stiffness changes induced will be studied using a biaxial material tester. Specific aim 1 is designed to identify one or more "candidate eye drops" that will then be used for in vivo rabbit experiments. 2. Test the hypothesis that corneal cross-linking through nitro technology is tolerated by corneal cells. Primary cultures of rabbit endothelial cells, keratocytes, and epithelial cells will be grown in vitro. The cells will then be exposed to nitro compounds found to be efficacious for cross-linking. Cytotoxicity will be evaluated using standard apoptosis and necrosis assays. The results from this aim will establish the in vitro toxicity level of nitro compounds to corneal cells. Specific aim 3. Test the hypothesis that corneal cross-linking through nitro technology has efficacy and is safe for the living eye. 33 young adult female New Zealand white rabbits will undergo daily application (for 1-6wks) of the topical nitro solution to the right eye with the fellow eye serving as a control. The animals will undergo serial examinations using 3 non-invasive instruments, a corneal topographer, in vivo confocal microscope, and applanation tonometer. Post-mortem, biomechanical strength testing, histology, and SEM will be performed. The results of this aim will establish the in vivo efficacy and safety of this technology and will dictate the feasibility of a human phase I trial. PUBLIC HEALTH RELEVANCE This research aims to develop a simple, new treatment for diseases of corneal destabilization which include keratoconus and post-surgical keratectasias. The latter conditions are devastating, long-term (5-10 years) complications following LASIK procedures. They were previously unpredicted and are currently of unknown epidemiologic proportion.
描述(由申请人提供):在圆锥角膜和LASIK术后角膜扩张的治疗方面取得了重大突破。德国Wollensak、Spoerl和Seiler小组最近的研究表明,通过应用核黄素和紫外线(UVR)交联角膜胶原蛋白可以限制圆锥角膜患者的视力丧失。尽管取得了这些成功,但UVR疗法带来了伴随的风险,特别是与紫外线照射有关的风险。因此,FDA最近才批准在美国进行临床试验。本申请的主旨是使用新技术开发角膜胶原交联的替代方法。我们最近的研究表明,胶原蛋白通过与亚硝酸盐和相关试剂反应而交联,可诱导与UVR治疗相当的角膜变化。此外,已发表的毒性/致突变性研究表明这些药物具有良好的安全性特征。因此,硝基技术可用作UVR治疗的“眼药水替代品”。相对于当前UVR疗法的潜在优势包括省略紫外线暴露、治疗薄角膜的能力、较少的患者不适、易于自我施用、剂量反应效应以及治疗周边角膜的能力。该项目旨在为将这项技术快速转化为治疗奠定基础。具体来说,我们将:1。确定在模拟人类角膜的条件下(即pH 7.4和34 ℃)使用硝基技术交联角膜的有效方法。新鲜兔眼和人眼库角膜将在各种条件下孵育,包括1。亚硝酸盐与催化剂和2.替代亚硝酸盐相关试剂。热收缩温度的测定将用于确定交联功效。将使用双轴材料测试仪研究引起的刚度变化。具体目标1旨在确定一种或多种“候选滴眼液”,然后将其用于兔体内实验。2.测试通过硝基技术的角膜交联被角膜细胞耐受的假设。兔内皮细胞、角膜细胞和上皮细胞的原代培养物将在体外生长。然后将细胞暴露于发现对交联有效的硝基化合物。将使用标准细胞凋亡和坏死试验评价细胞毒性。该目的的结果将确定硝基化合物对角膜细胞的体外毒性水平。具体目标3。验证通过硝基技术进行角膜交联对活体眼有效且安全的假设。33只年轻成年雌性新西兰白色兔的右眼将接受每日局部硝基溶液的应用(持续1- 6周),对侧眼作为对照。将使用3种非侵入性仪器(角膜地形图仪、体内共焦显微镜和压平眼压计)对动物进行系列检查。将进行尸检、生物力学强度试验、组织学和SEM。这一目标的结果将确定该技术的体内疗效和安全性,并将决定人体I期试验的可行性。公共卫生相关性本研究旨在开发一种简单的、新的治疗角膜不稳定疾病的方法,包括圆锥角膜和手术后角膜扩张。后一种情况是LASIK手术后毁灭性的长期(5-10年)并发症。它们以前是不可预测的,目前的流行病学比例未知。

项目成果

期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nitroalcohol Induced Hydrogel Formation in Amine-Functionalized Polymers.
  • DOI:
    10.1002/app.31944
  • 发表时间:
    2010-07-15
  • 期刊:
  • 影响因子:
    3
  • 作者:
    Solomon, Marissa R.;O'Connor, Naphtali A.;Paik, David C.;Turro, Nicholas J.
  • 通讯作者:
    Turro, Nicholas J.
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DAVID C PAIK其他文献

DAVID C PAIK的其他文献

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{{ truncateString('DAVID C PAIK', 18)}}的其他基金

Therapeutic Corneal Cross-linking Using Aliphatic Beta-Nitroalcohols
使用脂肪族 β-硝基醇进行治疗性角膜交联
  • 批准号:
    8616069
  • 财政年份:
    2011
  • 资助金额:
    $ 19.8万
  • 项目类别:
Therapeutic Corneal Cross-linking Using Aliphatic Beta-Nitroalcohols
使用脂肪族 β-硝基醇进行治疗性角膜交联
  • 批准号:
    8228144
  • 财政年份:
    2011
  • 资助金额:
    $ 19.8万
  • 项目类别:
Therapeutic Corneal Cross-linking Using Aliphatic Beta-Nitroalcohols
使用脂肪族 β-硝基醇进行治疗性角膜交联
  • 批准号:
    8435517
  • 财政年份:
    2011
  • 资助金额:
    $ 19.8万
  • 项目类别:
Therapeutic Corneal Cross-Linking Using Formaldehyde Releasing Agents
使用甲醛释放剂进行治疗性角膜交联
  • 批准号:
    9106336
  • 财政年份:
    2011
  • 资助金额:
    $ 19.8万
  • 项目类别:
Therapeutic Corneal Cross-linking Using Aliphatic Beta-Nitroalcohols
使用脂肪族 β-硝基醇进行治疗性角膜交联
  • 批准号:
    8040254
  • 财政年份:
    2011
  • 资助金额:
    $ 19.8万
  • 项目类别:
Therapeutic Corneal Cross-Linking Using Formaldehyde Releasing Agents
使用甲醛释放剂进行治疗性角膜交联
  • 批准号:
    9233107
  • 财政年份:
    2011
  • 资助金额:
    $ 19.8万
  • 项目类别:
A Novel Treatment for Keratoconus and Keratectasias using Nitro Technology
使用硝基技术治疗圆锥角膜和角膜扩张症的新方法
  • 批准号:
    7588578
  • 财政年份:
    2009
  • 资助金额:
    $ 19.8万
  • 项目类别:
NITRITE/COLLAGEN REACTIONS IN AGING AND SMOKING
衰老和吸烟中的亚硝酸盐/胶原蛋白反应
  • 批准号:
    6629663
  • 财政年份:
    2000
  • 资助金额:
    $ 19.8万
  • 项目类别:
NITRITE/COLLAGEN REACTIONS IN AGING AND SMOKING
衰老和吸烟中的亚硝酸盐/胶原蛋白反应
  • 批准号:
    6044277
  • 财政年份:
    2000
  • 资助金额:
    $ 19.8万
  • 项目类别:
NITRITE/COLLAGEN REACTIONS IN AGING AND SMOKING
衰老和吸烟中的亚硝酸盐/胶原蛋白反应
  • 批准号:
    6509365
  • 财政年份:
    2000
  • 资助金额:
    $ 19.8万
  • 项目类别:

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