Zeiss LSM 710 confocal microscope

描述（由申请人提供）：该提案要求支持购买蔡司LSM 710共聚焦显微镜，这将是纽约大学医学院五个主要用户实验室工作的关键共享资源，也是更广泛的纽约大学医学院社区外部用户的宝贵资源。主要用户实验室的项目主要由6个NIH R 01赠款资助，这些项目涉及胚胎发育期间细胞类型如何指定以及这些不同类型的细胞如何重新排列以形成功能器官。这些研究包括（1）确定斑马鱼胚胎心脏分化和形态发生的机制（Yelon）;（2）研究果蝇眼睛的图案和细胞命运决定中的全局转录控制的作用（Treisman）;（3）确定C. elegans早期胚胎细胞和上皮细胞的研究（Nance）;（4）了解C. elegans分化和增殖（Hubbard）;（5）破译斑马鱼脉管系统如何形成模式（Torres-Vazquez）。每个项目都严重依赖于使用荧光显微镜来观察细胞的形状和运动，并检查蛋白质的亚细胞定位;因为成像的胚胎或组织相对较厚，所以需要共聚焦显微镜来去除平面外的荧光。要求能够使用光活化蛋白质并同时成像GFP和mCherry的辅助激光器，以实现新的和强大的实时成像实验。主要用户PI是具有相当多的共聚焦显微镜经验的高级和初级教师的组合。他们将与Image Core Facility的负责人Alice Liang博士一起合作支持和操作共聚焦显微镜。梁博士还将监督整个纽约大学医学院的外部研究人员对该范围的培训和使用。公共卫生相关性：本提案中要求的显微镜对于推进纽约大学医学院正在进行的NIH资助项目的研究至关重要，并且所要求的辅助激光线将使新的研究线成为可能。这些项目共同使用模型生物来解决发育生物学中的基本问题，包括细胞如何形成模式和组织以形成器官。对这些过程的基本了解对于理解人类发展和疾病至关重要。

DESCRIPTION (provided by applicant): This proposal requests support for the purchase of a Zeiss LSM 710 confocal microscope, which will be a shared resource crucial to the work of five Major User laboratories at NYU School of Medicine as well as a valuable resource to outside users in the wider NYU School of Medicine community. The projects of the Major User labs, which are funded predominantly by 6 NIH R01 grants, address both how cell types are specified during embryonic development and how these different types of cells are rearranged to form functional organs. These studies include (1) identifying mechanisms of heart differentiation and morphogenesis in zebrafish embryos (Yelon); (2) investigating patterning of the Drosophila eye and the role of global transcriptional control in cell fate decisions (Treisman); (3) determining how C. elegans early embryonic cells and epithelial cells polarize (Nance); (4) learning how germ-line stem cells in C. elegans differentiate and proliferate (Hubbard); and (5) deciphering how the zebrafish vasculature becomes patterned (Torres-Vazquez). Each project relies heavily on the use of fluorescence microscopy to visualize cell shapes and movements and to examine the subcellular localization of proteins; because the embryos or tissues imaged are relatively thick, confocal microscopy is required to remove out-of-plane fluorescence. Accessory lasers that enable use of photoactivatable proteins and simultaneous imaging of GFP and mCherry are requested to enable new and powerful types of live imaging experiments. The Major User PIs are a combination of senior and junior faculty with considerable confocal microscopy experience. Together with Dr. Alice Liang, head of the Image Core Facility, they will cooperate in supporting and operating the confocal microscope. Dr. Liang will also oversee training and use of the scope by outside investigators throughout NYU School of Medicine. PUBLIC HEALTH RELEVANCE: The microscope requested in this proposal will be crucial in advancing the research of NIH-funded projects ongoing at NYU School of Medicine, and the requested accessory laser lines will enable new lines of investigation. Collectively the projects use model organisms to address fundamental questions in developmental biology, including how cells are patterned and organized to form organs. A basic understanding of these processes is critical to understanding human development and disease.