BD Biosciences Special Order LSRII

BD Biosciences 特别订单 LSRII

• 批准号: 7591572
• 负责人: Ellen A Lumpkin
• 金额: $ 42.98万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7591572
• 关键词: AIDS/HIV problem; Aging; Aging-Related Process; Bacteria; Cancer Biology; Cardiovascular system; Cell Separation; Cell surface; Cells; Communities; Congenital Heart Defects; Cyan Fluorescent Protein; Cytometry; Detection; Development; Dyes; Fluorescein; Fluoresceins; Fluorochrome; Funding; Green Fluorescent Proteins; Human; Immunology; Individual; Lasers; Malignant Neoplasms; Medical center; Medicine; Phycoerythrin; Process; Proteins; Regenerative Medicine; Research; Research Personnel; Research Project Grants; Societies; Stroke; Texas; Tube; United States National Institutes of Health; Viola; Yeasts; allophycocyanin; base; college; design; detector; flexibility; fluorophore; instrument; particle; public health relevance; red fluorescent protein; solid state; stem cell therapy

DESCRIPTION (provided by applicant): This application is for funds to purchase a BD Biosciences Special Order LSRII quadruple-laser FACS analyzer that will be situated in the new Cytometry and Cell Sorting Core at Baylor College of Medicine (BCM). This instrument has been selected to serve the purposes of over 16 investigators involved in over 18 NIH- funded projects. This instrument has been designed to serve two key purposes in our community. First, many BCM investigators require the capacity to analyze on multiple fluorescent markers concomitantly with multiple cell surface markers. Our four-laser instrument will allow analysis based on multiple parameters simultaneously such as mCherry, cyan fluorescent protein, yellow fluorescent protein, and allophycocyanin (APC) or sytox red. It will have a 561-nm solid-state laser, allowing detection of red fluorescent protein derivatives such as mCherry and increasing the sensitivity of detecting commonly used probes such as phycoerythrin. It will also be equipped with a 405-nm solid-state laser enabling the use of violet sensitive probes including cyan fluorescent protein and the fluorochrome Pacific Blue. Additionally, a 640-nm laser will allow detection of fluorochromes such as APC and its conjugates as well as the viability dye sytox red, which will give added power and flexibility to all investigators needing multiple easy-to-use parameters for analysis. Finally, the instrument will be equipped with a 488-nm solid-state laser enabling detection of standard fluorophores including green fluorescent protein, yellow fluorescent protein and fluorescein. The second key purpose for this flow analyzer is small-particle detection to study bacteria and yeast, which is critical for four groups of investigators at BCM. Thus, the instrument will include a forward scatter photo-multiplier tube detector enabling small particle detection and analysis, directly enhancing the research using bacteria and yeast. This instrument will afford maximum capabilities as well as maximum flexibility to best serve the evolving needs of the diverse BCM investigators and Texas Medical Center research community. This instrument is essential for the broad base of health-related research at BCM, including projects aimed at developing new therapies in regenerative medicine, cancer, HIV/AIDS, immunology and aging, which will contribute to the overall wellness of our society. Public Health Relevance: Our research at the Baylor College of Medicine requiring a four-laser LSRII flow cytometer will specifically study processes within cells allowing us to better understand stem cell therapies, aging processes, cancer biology and cardiovascular development. The requested LSRII flow cytometer is critical for determining the complexity and differences between individual cells, which is essential for our research projects aimed at developing new treatments or cures for serious human illnesses including cancer, heart abnormalities, stroke and HIV/AIDS.

描述(由申请人提供)：此申请是为了筹集资金购买BD Biosciences Special Order LSRII四路激光FACS分析仪，该分析仪将安装在贝勒医学院(BCM)新的细胞测量和细胞分类中心。该仪器已被选为参与NIH资助的18个项目的16名以上调查人员的目的。这个工具的设计是为了在我们的社区中服务于两个主要目的。首先，许多BCM研究人员需要同时分析多个荧光标记和多个细胞表面标记的能力。我们的四激光仪器将允许基于多个参数同时进行分析，例如mCherry、青色荧光蛋白、黄色荧光蛋白和别藻蓝蛋白(APC)或Sytox红。它将拥有561 nm的固态激光器，可以检测到mCherry等红色荧光蛋白质衍生物，并提高了检测常用探针(如藻红蛋白)的灵敏度。它还将配备一个405 nm的固态激光器，可以使用紫光敏感的探针，包括青色荧光蛋白和荧光太平洋蓝。此外，640 nm激光将允许检测APC及其结合物等荧光染料以及活性染料SYTOX红，这将为所有需要多个易于使用的参数进行分析的研究人员提供更大的能力和灵活性。最后，该仪器将配备488 nm固体激光器，能够检测包括绿色荧光蛋白、黄色荧光蛋白和荧光素在内的标准荧光团。这种流动分析仪的第二个关键用途是研究细菌和酵母的小颗粒检测，这对BCM的四组研究人员来说是至关重要的。因此，该仪器将包括一个前向散射光电倍增管探测器，能够检测和分析小颗粒，直接加强使用细菌和酵母的研究。该仪器将提供最大的能力以及最大的灵活性，以最大限度地满足多样化的BCM研究人员和德克萨斯医学中心研究社区不断变化的需求。这一工具对于BCM与健康相关的广泛研究基础至关重要，包括旨在开发再生医学、癌症、艾滋病毒/艾滋病、免疫学和老龄化方面的新疗法的项目，这将有助于我们社会的整体健康。公共卫生相关性：我们在贝勒医学院的研究需要四激光LSRII流式细胞仪，该研究将专门研究细胞内的过程，使我们能够更好地了解干细胞疗法、衰老过程、癌症生物学和心血管发育。所要求的LSRII流式细胞仪对于确定单个细胞之间的复杂性和差异至关重要，这对于我们旨在开发治疗癌症、心脏异常、中风和艾滋病毒/艾滋病等严重人类疾病的新疗法或治疗方法的研究项目至关重要。