BD Biosciences Special Order LSRII

描述（由申请人提供）：本申请用于购买BD Biosciences Special Order LSRII四激光FACS分析仪的资金，该分析仪将位于贝勒医学院（Baylor College of Medicine）的新细胞计数和细胞分选核心。该工具已被选定为超过16名研究人员的目的，参与超过18个NIH资助的项目。这个工具的设计是为了在我们的社会中达到两个关键目的。首先，许多荧光标记物的研究者需要同时分析多种细胞表面标记物的能力。我们的四激光仪器将允许同时基于多个参数进行分析，例如mCherry，青色荧光蛋白，黄色荧光蛋白和别藻蓝蛋白（APC）或sytox red。它将具有561 nm的固态激光器，可以检测红色荧光蛋白衍生物，如mCherry，并提高检测常用探针的灵敏度，如藻红蛋白。它还将配备一个405纳米的固态激光器，能够使用包括青色荧光蛋白和荧光染料太平洋蓝在内的紫色敏感探针。此外，640 nm激光将允许检测荧光染料，如APC及其结合物以及活力染料sytox red，这将为所有需要多个易于使用的参数进行分析的研究人员提供额外的能力和灵活性。最后，该仪器将配备488 nm固态激光器，能够检测标准荧光团，包括绿色荧光蛋白、黄色荧光蛋白和荧光素。该流动分析仪的第二个关键目的是研究细菌和酵母的小颗粒检测，这对研究中心的四组研究人员至关重要。因此，该仪器将包括一个前向散射光电倍增管探测器，能够进行小颗粒探测和分析，直接加强使用细菌和酵母的研究。该仪器将提供最大的能力以及最大的灵活性，以最好地满足不同的研究人员和德克萨斯州医学中心研究社区不断变化的需求。这一工具对于生物医学研究所广泛的健康相关研究基础至关重要，包括旨在开发再生医学、癌症、艾滋病毒/艾滋病、免疫学和老龄化新疗法的项目，这将有助于我们社会的整体福祉。公共卫生相关性：我们在贝勒医学院的研究需要四激光LSRII流式细胞仪，将专门研究细胞内的过程，使我们能够更好地了解干细胞疗法、衰老过程、癌症生物学和心血管发育。所要求的LSRII流式细胞仪对于确定单个细胞之间的复杂性和差异至关重要，这对于我们的研究项目至关重要，这些研究项目旨在开发新的治疗方法或治愈严重的人类疾病，包括癌症，心脏异常，中风和艾滋病毒/艾滋病。

DESCRIPTION (provided by applicant): This application is for funds to purchase a BD Biosciences Special Order LSRII quadruple-laser FACS analyzer that will be situated in the new Cytometry and Cell Sorting Core at Baylor College of Medicine (BCM). This instrument has been selected to serve the purposes of over 16 investigators involved in over 18 NIH- funded projects. This instrument has been designed to serve two key purposes in our community. First, many BCM investigators require the capacity to analyze on multiple fluorescent markers concomitantly with multiple cell surface markers. Our four-laser instrument will allow analysis based on multiple parameters simultaneously such as mCherry, cyan fluorescent protein, yellow fluorescent protein, and allophycocyanin (APC) or sytox red. It will have a 561-nm solid-state laser, allowing detection of red fluorescent protein derivatives such as mCherry and increasing the sensitivity of detecting commonly used probes such as phycoerythrin. It will also be equipped with a 405-nm solid-state laser enabling the use of violet sensitive probes including cyan fluorescent protein and the fluorochrome Pacific Blue. Additionally, a 640-nm laser will allow detection of fluorochromes such as APC and its conjugates as well as the viability dye sytox red, which will give added power and flexibility to all investigators needing multiple easy-to-use parameters for analysis. Finally, the instrument will be equipped with a 488-nm solid-state laser enabling detection of standard fluorophores including green fluorescent protein, yellow fluorescent protein and fluorescein. The second key purpose for this flow analyzer is small-particle detection to study bacteria and yeast, which is critical for four groups of investigators at BCM. Thus, the instrument will include a forward scatter photo-multiplier tube detector enabling small particle detection and analysis, directly enhancing the research using bacteria and yeast. This instrument will afford maximum capabilities as well as maximum flexibility to best serve the evolving needs of the diverse BCM investigators and Texas Medical Center research community. This instrument is essential for the broad base of health-related research at BCM, including projects aimed at developing new therapies in regenerative medicine, cancer, HIV/AIDS, immunology and aging, which will contribute to the overall wellness of our society. Public Health Relevance: Our research at the Baylor College of Medicine requiring a four-laser LSRII flow cytometer will specifically study processes within cells allowing us to better understand stem cell therapies, aging processes, cancer biology and cardiovascular development. The requested LSRII flow cytometer is critical for determining the complexity and differences between individual cells, which is essential for our research projects aimed at developing new treatments or cures for serious human illnesses including cancer, heart abnormalities, stroke and HIV/AIDS.