FLOW CYTOMETRY

流式细胞术

• 批准号: 8180977
• 负责人: Ellen A Lumpkin
• 金额: $ 12.89万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-17 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8180977
• 关键词: Address; Arts; Cancer Center; Cancer Center Support Grant; Cell Count; Cell Cycle Progression; Cell Separation; Cells; Collaborations; Color; Communities; Consultations; Cytometry; DNA; Data Analyses; Ensure; Equipment; Flow Cytometry; Flow Cytometry Shared Resource; Fluorescence; Fluorescence-Activated Cell Sorting; Foundations; Funding; Future; Goals; Grant; Hour; Housing; Institution; Magnetism; Medicine; Minnesota; Mission; Optics; Pediatric Hospitals; Preparation; Protocols documentation; Recording of previous events; Recruitment Activity; Reporter; Research; Research Personnel; Resource Sharing; Resources; Sampling; Seeds; Services; Site; Sorting - Cell Movement; Surveys; Technology; Texas; Time; Training; Transcriptional Regulation; Tumor Suppressor Genes; United States National Institutes of Health; angiogenesis; anticancer research; base; cancer stem cell; cancer therapy; cell separator; college; cost; cost effective; experience; improved; instrument; instrumentation; magnetic cell separation; meetings; member; neoplastic cell; novel; operation; programs; repaired; square foot; transforming virus

The goal of the Flow Cytometry Shared Resource to provide users with cost-effective instrumentation, expertise and training for cell sorting and analysis. This technology continues to develop at a rapid pace, especially with the advent of novel fluorescent reporters, increased computational capacity and more cost-effective optical equipment. To meet our members' increasing demands for state-of-the-art flow cytometry, the DLDCC and BCM administration collaborated to create an entirely new flow cytometry facility in 2007. Renovation, operating costs and instrumentation has been supported by $1.7 million in BCM institutional funds and >$600,000 in DLDCC funds. The revamped Facility is housed in newly renovated, centrally located space, which is available to trained users 24 h a day via key-card access. State-of-the-art instrumentation, all of which has been purchased in the last three years, includes two fully loaded florescence-activated cell sorters, three flow analyzers and a magnetic cell separator. The Resource is directed by Dr, Ellen A. Lumpkin, who has over nine years of experience in flow cytometry, and Mr. Joel Sederstrom, who was recruited from the Univ. of Minnesota's Cancer Center Flow Cytometry Core in a national search. To ensure optimal use of services, the Resource provides consultations, training and protocols for sample preparation, flow analysis and cell sorting. The Resource is also staffed with two full-time experienced flow cytometrists who perform operator-assisted cytometry, and assist users with data analysis. With the Resource's improved services and capacity, FACS sorting has increased by >500% and FACS analysis has increased 160% among Cancer Center members. At present, the Resource operates near 100% of its capacity, with 78% of usage occupied by 65 Cancer Center investigators whose membership spans all Scientific Programs. Future plans include further expanding services by recruiting an additional cytometrist and by including a second site at our affiliated institution, Texas Children's Hospital Cancer Center.

流式细胞术共享资源的目标是为用户提供具有成本效益的仪器，专业知识和细胞分选和分析培训。该技术继续快速发展，特别是随着新型荧光报告分子的出现，计算能力的提高和更具成本效益的光学设备。 为了满足我们的会员对最先进的流式细胞仪日益增长的需求，DLDCC和FDA管理部门于2007年合作创建了一个全新的流式细胞仪设施。翻修、运营费用和仪器得到了170万美元的可持续发展机构资金和60多万美元的荒漠化、土地退化和气候变化协调委员会资金的支持。改造后的设施位于新装修的中心位置，经过培训的用户可以通过钥匙卡每天24小时进入。最先进的仪器，所有这些都有 在过去三年中购买了一台新的细胞分选仪，包括两台满载的荧光激活细胞分选仪、三台流式分析仪和一台磁性细胞分离器。该资源由Ellen A博士指导。伦普金，他在流式细胞术方面有九年多的经验，乔尔·塞德斯特罗姆先生，他是在全国范围内从明尼苏达大学癌症中心流式细胞术核心招募来的。为确保服务得到最佳利用，该资源为样品制备、流动分析和细胞分选提供咨询、培训和规程。该资源还配备了两名全职的经验丰富的流式细胞仪，他们进行操作员辅助细胞仪，并协助用户进行数据分析。 随着资源的服务和能力的改善，癌症中心成员的流式细胞仪分选增加了500%以上，流式细胞仪分析增加了160%。目前，该资源的运作接近100%的能力，78%的使用被65名癌症中心的研究人员占用，他们的成员涵盖了所有的科学计划。未来的计划包括进一步扩大服务，招募额外的细胞仪，并包括在我们的附属机构，得克萨斯州儿童医院癌症中心的第二个网站。