Solid State NMR Console and Probe Upgrade for Biophysical and Biomaterial Studie

描述(由申请人提供)：建议通过替换过时的、自制的单个R.F.来扩大在华盛顿大学进行现代多核固态核磁共振实验的能力。通道500 MHz核磁共振控制台与商业三通道固态核磁共振控制台。近年来，参与独立或与加里·德罗布尼的研究小组合作进行固态核磁共振实验的小组数量有所增加。加布里埃勒·瓦拉尼教授(化学/生物化学)的研究小组使用固态和溶液核磁共振来研究核酸内部运动的性质，以及这些运动在核酸-蛋白质识别中可能发挥的作用。Patrick Stayton教授(生物工程)和Gary Drobny教授(化学)使用固态核磁共振研究生物材料界面上蛋白质的结构和动力学，Rachel Klevit(生物化学)使用固态研究微晶蛋白质的结构，这些微晶蛋白质很难用溶液核磁共振方法进行研究。加里·德罗布尼还使用固态核磁共振研究生物材料表面的多肽结构。为了适应这些研究计划，华盛顿大学的固态核磁共振仪器的可用性需要扩大。我们建议以一种具有成本效益的方式来实现这一点，方法是用三重共振MAS探头增强Bruker DMX-750光谱仪的能力。并不是所有的固态核磁共振实验都需要在17.6T下进行，因此，为了适应大多数想要进行固态核磁共振的群体，我们建议替换单通道自制的500 MHz。带有商用三通道核磁共振控制台的控制台。与公共健康相关：了解生物分子的结构和动力学是了解其功能的基础。本申请中要求的固态核磁共振仪器的主要用户调查：1)与针对HIV病毒RNA的药物设计相关的蛋白质-RNA相互作用的性质；2)蛋白质-生物材料表面相互作用的性质，以期得出生物传感器和医疗植入物涂层的设计原则；以及3)结合和隔离未折叠蛋白质的热休克蛋白质的结构。

DESCRIPTION (provided by applicant): It is proposed to expand the capability to perform modern multi-nuclear solid state NMR experiments at the University of Washington by replacing an obsolete, home-built single r.f. channel 500 MHz NMR console with a commercial triple channel solid state NMR console. The number of groups involved in performing solid state NMR experiments independently or collaboratively with Gary Drobny's research group has increased in recent years. The research group of Professor Gabriele Varani (Chemistry/Biochemistry) uses both solid state NMR and solution NMR to investigate the nature of internal motions in nucleic acids and the role that these motions might play in nucleic acid-protein recognition. Professor Patrick Stayton (Bioengineering), and Professor Gary Drobny (Chemistry) use solid state NMR to study the structure and dynamics of proteins at biomaterial interfaces, and Rachel Klevit (Biochemistry) uses solid state to study the structure of microcrystalline proteins that are intractable to solution NMR methods. Gary Drobny also uses solid state NMR to study peptide structure on biomaterial surfaces. To accommodate these research programs, the availability of solid state NMR instrumentation at UW needs to be expanded. We propose to do this in a cost-effective way by augmenting the capabilities of the Bruker DMX-750 spectrometer with a triple resonance MAS probe. Not all solid state NMR experiments need to be performed at 17.6 T, so to accommodate the majority of groups wanting to do solid state NMR we propose to replace a single channel homebuilt 500 MHz. console with a commercial triple channel NMR console. PUBLIC HEALTH RELEVANCE: Knowledge of the structure and dynamics of bio-molecules is fundamental to understanding their function. The Major-Users of the Solid State NMR Instrumentation requested in this application investigate: 1) the nature of protein-RNA interactions with relevance to the design of drugs that target HIV viral RNA; 2) the nature of protein-biomaterial surface interactions with a view to derive design principles for biosensors, and coatings for medical implants; and 3) the structure of heat shock proteins, that bind and sequester unfolded proteins.