Shared Instrumentation Grant: LTQ Linear Ion Trap Mass Spectrometer

共享仪器资助：LTQ 线性离子阱质谱仪

• 批准号: 7595384
• 负责人: Jeffrey D Esko
• 金额: $ 42.01万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595384
• 关键词: Animal Model; Binding Proteins; Biological; Biological Process; Blood capillaries; California; Categories; Communities; Contract Services; Disease; Electrons; Freezing; Funding; Glycobiology; Glycoconjugates; Glycolipids; Glycosaminoglycans; Glycosides; Grant; Heterogeneity; High Pressure Liquid Chromatography; Housing; Hypersensitivity; Immunology; Income; Institutes; Ions; Lead; Lectin; Mass Spectrum Analysis; Medical Research; Methods; Operative Surgical Procedures; Patients; Pharmacologic Substance; Play; Polysaccharides; Research Training; Role; Sampling; Scientist; Sialic Acids; Source; Specificity; Structure; Therapeutic Agents; Universities; base; capillary; cost; experience; falls; instrument; instrumentation; liquid chromatography mass spectrometry; mass spectrometer; meetings; member; molecular marker; public health relevance

DESCRIPTION (provided by applicant): Glycoconjugates play crucial roles in many fundamental biological processes, and changes in glycan structure correlate with certain diseases and disorders. The large number of biological activities attributed to glycans is due in part to their interaction with glycan-binding proteins or lectins, and the interaction often occurs with great specificity with respect to structure of the glycan. Mass spectrometry, especially liquid chromatography mass spectrometry (LC/MS), is playing an increasingly important role in the analysis of glycan structure. Here we request funds to purchase a Thermo Electron Finnigan LTQ XL ion trap mass spectrometer (MS) with electrospray sources, equipped with an Agilent 1200 capillary HPLC. The instrument will meet the needs of 4 primary projects and 12 secondary projects carried out by multiple users based at the University of California, San Diego, the University of California, Irvine, and The Burnham Institute for Medical Research, the La Jolla Institute for Allergy and Immunology, and Zacharon Pharmaceuticals, Inc. The projects fall into four major categories: (1) Analysis of N- and O-glycans (H. Freeze, L. Parsons, W. Dillman, M. Fukuda, M.N. Fukuda, A. Varki); (2) Sialic acid heterogeneity (A. Varki, D. Broide, V. Nizet); (3) Glycosaminoglycan analysis (J. Esko, B. Crawford, Y. Tor, Y. Yamaguchi, R. Warrior, R. Gallo); and (4) Glycolipids and glycosides (B. Crawford, R. Aroian, M. Kronenberg, J. Esko). The requested instrument will be housed in the Glycotechnology Core and administered through the Glycobiology Research and Training Center at the University of California, San Diego. Priority will be given to the primary projects and secondary users on a recharge basis, with use by other members of the glycobiology community at large when available. The instrument will be operated and maintained by Drs. Anup Datta (Core Director), Roger Lawrence (Project Scientist), and Sulabha Argade all of whom have experience in mass spectrometry as applied to glycans. The cost of instrument operation, supplies, and service contract will be shared by the users from recharge income. PUBLIC HEALTH RELEVANCE: Structural studies of glycans can lead to a better understanding of how they interact with glycan-binding proteins, which in turn can lead to the discovery of molecular markers for diseases and therapeutic agents. Liquid Chromatography Mass Spectrometry is one of the most thorough means for structurally analyzing glycans and glycoconjugates, being more robust, sensitive and reliable than other methods. It is particularly useful in analyzing biological samples from model organisms and patients, without necessitating large amounts of highly purified samples.

描述（由申请人提供）：糖缀合物在许多基本的生物学过程中起着至关重要的作用，而聚糖结构的变化与某些疾病和疾病相关。归因于聚糖的大量生物学活性部分是由于它们与聚糖结合蛋白或凝集素的相互作用，并且相互作用通常相对于聚糖的结构而具有很强的特异性。质谱法，尤其是液相色谱质谱法（LC/MS），在分析聚糖结构中起着越来越重要的作用。在这里，我们要求资金购买带有电喷雾源的Thermo Electron Finnigan LTQ XL离子陷阱质谱仪（MS），配备了Agilent 1200毛细管HPLC。该工具将满足加州大学，圣地亚哥大学，加利福尼亚大学，欧文大学和伯纳姆医学研究所，拉霍亚过敏和免疫学研究所以及Zacharon Pharmaceuticals，Inc的多个用户进行的4个主要项目和12个次要项目的需求。 Dillman，M。Fukuda，M.N。Fukuda，A。Varki）； （2）唾液酸异质性（A. Varki，D。Broide，V。Nizet）； （3）糖胺聚糖分析（J. Esko，B。Crawford，Y。Tor，Y。Yamaguchi，R。Warrior，R。Gallo）； （4）糖脂和糖苷（B. Crawford，R。Aroian，M。Kronenberg，J。Esko）。所请求的仪器将安置在糖精技术核心中，并通过加州大学圣地亚哥分校的糖生物学研究与培训中心进行管理。优先级将以充电为基础向主要项目和二级用户提供优先级，并在可用时使用整个糖生物学社区的其他成员。该仪器将由Drs操作和维护。 Anup Datta（核心总监），Roger Lawrence（项目科学家）和Sulabha Argade都具有应用于Glycans的质谱经验。仪器操作，供应和服务合同的成本将由用户从充值收入中分担。公共卫生相关性：聚糖的结构研究可能会更好地了解它们与聚糖结合蛋白的相互作用，这反过来又可能导致发现疾病和治疗剂的分子标记。液相色谱质谱法是结构分析聚糖和糖缀合物的最彻底手段之一，比其他方法更健壮，敏感和可靠。它在分析来自模型生物和患者的生物样品时特别有用，而无需大量高度纯化的样品。