Live Cell Confocal Microscope for FRAP/PA

用于 FRAP/PA 的活细胞共焦显微镜

• 批准号: 7595583
• 负责人: Duane A. Compton
• 金额: $ 50万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595583
• 关键词: Actins; Aneuploidy; Arts; Biochemistry; Biological Sciences; Cell physiology; Cells; Chromosome Segregation; Communities; Computer software; Diabetes Mellitus; Disease; Etiology; Goals; Head; Health; Insulin; Lasers; Life; Light; Measurement; Membrane Biology; Membrane Protein Traffic; Microscope; Minor; Molecular; Neoplasm Metastasis; Photobleaching; Phototoxicity; Process; Research Personnel; Research Project Grants; Resolution; Technology; Time; college; human disease; insight; instrument; medical schools; neoplastic cell; nucleocytoplasmic transport; photoactivation; photonics; public health relevance

DESCRIPTION (provided by applicant): The goal of this proposal is to acquire a spinning-disc confocal microscope equipped for FRAP and photoactivation to be used for the measurement of dynamic processes in live cells. The major and minor users of this instrument are in the Departments of Biochemistry and Biological Sciences at Dartmouth Medical School and College. Their research projects are aimed at understanding molecular mechanisms of fundamental cellular processes such as chromosome segregation, actin organization, nuclear transport, and membrane trafficking. Satisfactory completion of the stated goals of the NIH-sponsored projects of these users requires examination of dynamic cellular processes in live cells with high spatial and temporal resolution and minimal phototoxicity. The spinning-disc confocal microscope described in this proposal is a state of the art instrument that satisfies these needs by using proven technology (Yokogawa spinning-disc confocal head; Nikon inverted microscope; Photonic Instruments photobleaching/activating laser; Improvision software) to provide real-time confocality (high spatial resolution) at rapid rates (high temporal resolution) with minimal light exposure. It would represent the only instrument of its kind to serve the entire Dartmouth life science community. PUBLIC HEALTH RELEVANCE: The goal of this proposal is to gain insight into the molecular mechanisms that underlie various human disease states such as tumor cell aneuploidy, metastasis, and membrane biology related to health issues such as insulin action in diabetes. The unique attributes of the microscope requested give the investigators unparalleled ability to observe dynamic processes in living cells, and insight into disease etiology can emerge from understanding the dynamics of these fundamental cell mechanisms.

描述（由申请人提供）：本提案的目标是获得一个旋转圆盘共聚焦显微镜，配备FRAP和光活化，用于测量活细胞中的动态过程。该仪器的主要和次要用户是在达特茅斯医学院的生物化学和生物科学系。他们的研究项目旨在了解基本细胞过程的分子机制，如染色体分离，肌动蛋白组织，核运输和膜运输。这些用户的NIH赞助的项目的既定目标的圆满完成需要在活细胞中检查动态细胞过程，具有高的空间和时间分辨率和最小的光毒性。本提案中描述的旋转盘共聚焦显微镜是一种最先进的仪器，通过使用成熟的技术（Yokogawa旋转盘共聚焦头; Nikon倒置显微镜; Photonic Instruments光漂白/激活激光器; Improvision软件）以最小的曝光量以快速（高时间分辨率）提供实时共聚焦（高空间分辨率）来满足这些需求。它将是服务于整个达特茅斯生命科学界的唯一同类仪器。公共卫生相关性：该提案的目标是深入了解各种人类疾病状态的分子机制，如肿瘤细胞非整倍性，转移和与糖尿病中胰岛素作用等健康问题相关的膜生物学。所要求的显微镜的独特属性为研究人员提供了无与伦比的观察活细胞动态过程的能力，并且可以通过了解这些基本细胞机制的动态来深入了解疾病病因。