JEOL JEM-1010 transmission electron microscope & Camera

描述(由申请人提供)：本建议书旨在收购 美国国立卫生研究院资助的新型透射电子显微镜和数码相机 研究和培训，以及其他研究，以及本科教育。 美国国立卫生研究院资助的蛋白质鉴定和特性研究 纺锤体微管负端组织的分子机制 哺乳动物细胞中的两极、金黄色葡萄球菌与哺乳动物的相互作用 细胞，以及霍乱弧菌对人类的定植都依赖于 高质量的透射电子显微镜检查和评价 实验。所选的透射式电子显微镜(TEM)是JEOL USA， JEM-Lolo电子显微镜，100KV显微镜，分辨率0.2 nm，高倾斜(+或 -60度)极片，而摄像机是Gatan，Inc.型号792 BioScan 1K 采用1K广角CCD摄像机。显微镜和照相机将被安置在 里佩尔电子显微镜设施(REMF)，一个机构资源，在那里它 将通过服务合同和全职工作人员进行培训， 研究援助和维护。现有的学院谘询委员会 将扩展至包括个人信息，以确保访问 特姆。瞬变电磁波？被替换的人是24岁，预计寿命没有 两年多，尽管已经连续签订了服务合同。 侦查人员的研究离不开原则的研究。 继续提供透射式电子显微镜。

DESCRIPTION (provided by applicant): This proposal is for the acquisition of a new transmission electron microscope and digital camera for use in NIH funded research and training, and other research, as well as undergraduate education. NIH-funded research on identification of proteins and characterization of molecular mechanisms involved in organizing microtubule minus ends at spindle poles in mammalian cells, interaction of Staphylococcus aureus with mammalian cells, and human colonization by the bacterium Vibrio cholera , all depend on high quality transmission electron microscopy for examination and evaluation of experiments. The transmission electron microscope (ThM) chosen is a JEOL USA, Inc. JEM-lOlO TEM, a 100KV microscope with 0.2 nm resolution, high tilt (+ or -60 degrees) pole piece, while the camera is a Gatan, Inc. Model 792 BioScan 1K by 1K wide angle CCD camera. The microscope and camera will be housed in the Rippel Electron Microscope Facility (REMF), an institutional resource, where it will be covered by service contract and a full time staff for training, research assistance and maintenance. The existing Faculty Advisory Committee will be extended to include the PIs for the purpose of ensuring access to the TEM. The TEM? to be replaced is 24 years old and has an anticipated life of no more than two years, despite having been continuously under service contract. The research of the principle investigators cannot be completed without continued availability of a transmission electron microscope.