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JEOL JEM-1010 transmission electron microscope & Camera

JEOL JEM-1010透射电子显微镜

基本信息

批准号：
6440877
负责人：
Duane A. Compton
金额：
$ 27.46万
依托单位：
DARTMOUTH COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-06-01 至 2003-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6440877
关键词：
Staphylococcus aureus Vibrio cholerae biomedical equipment purchase cell cell interaction charge coupled device camera microtubules spindle pole body transmission electron microscopy

项目摘要

DESCRIPTION (provided by applicant): This proposal is for the acquisition of a new transmission electron microscope and digital camera for use in NIH funded research and training, and other research, as well as undergraduate education. NIH-funded research on identification of proteins and characterization of molecular mechanisms involved in organizing microtubule minus ends at spindle poles in mammalian cells, interaction of Staphylococcus aureus with mammalian cells, and human colonization by the bacterium Vibrio cholera , all depend on high quality transmission electron microscopy for examination and evaluation of experiments. The transmission electron microscope (ThM) chosen is a JEOL USA, Inc. JEM-lOlO TEM, a 100KV microscope with 0.2 nm resolution, high tilt (+ or -60 degrees) pole piece, while the camera is a Gatan, Inc. Model 792 BioScan 1K by 1K wide angle CCD camera. The microscope and camera will be housed in the Rippel Electron Microscope Facility (REMF), an institutional resource, where it will be covered by service contract and a full time staff for training, research assistance and maintenance. The existing Faculty Advisory Committee will be extended to include the PIs for the purpose of ensuring access to the TEM. The TEM? to be replaced is 24 years old and has an anticipated life of no more than two years, despite having been continuously under service contract. The research of the principle investigators cannot be completed without continued availability of a transmission electron microscope.

描述（由申请人提供）：本建议书是为了收购一个 NIH资助使用的新型透射电子显微镜和数码相机研究和培训，以及其他研究，以及本科教育。美国国立卫生研究院资助的蛋白质鉴定和表征研究微管纺锤体负末端形成的分子机制哺乳动物细胞中的极，金黄色葡萄球菌与哺乳动物细胞的相互作用，细胞，以及霍乱弧菌在人类中的定植，都取决于高质量透射电子显微镜检查和评价实验选择的透射电子显微镜（ThM）是JEOL USA， Inc. JEM-1010 TEM，具有0.2nm分辨率的100 KV显微镜，高倾斜（+或 -60度）极片，而相机是Gatan，Inc. 792型BioScan 1 K 1 K广角CCD摄像机显微镜和摄像机将被安置在 Rippel电子显微镜设施（REMF）是一种机构资源，将通过服务合同和全职员工进行培训，研究协助和维护。现有的学院咨询委员会将扩展到包括PI，以确保访问透射电镜TEM？被替换的是24岁，预期寿命为两年多来，尽管一直在服务合同。主要研究人员的研究不可能完成，继续提供透射电子显微镜。