Xenogen IVIS Spectrum Imaging System

Xenogen IVIS 光谱成像系统

• 批准号: 7591501
• 负责人: WILLIAM A MALTESE
• 金额: $ 35万
• 依托单位: UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7591501
• 关键词: Adipocytes; Advisory Committees; Agonist; Animals; Applications Grants; Area; Bacterial Infections; Behavior; Biological Process; Biological Sciences; Bioluminescence; Cell Death; Cell Differentiation process; Contract Services; Data; Development; Diabetes Mellitus; Drug resistance; Equipment; Female; Fluorescence; Funding; Glioblastoma; Grant; Growth; Health Sciences; Image; Imaging technology; Immune response; Immunocompromised Host; Immunologic Surveillance; Infection; Inflammation; Insulin; Investigation; Laboratory Animal Medicine; Life; Lyme Disease; Malignant Neoplasms; Microscopy; Minor; Modeling; Neoplasm Metastasis; Neuroblastoma; Organism; Proteins; Regulation; Research; Research Personnel; Resource Sharing; Role; Schedule; Signaling Molecule; Skin; Steroid Receptors; Supervision; System; Therapeutic; Tissue-Specific Gene Expression; Tissues; Training; Transgenic Organisms; Transplantation; Universities; Work; cancer transplantation; cell type; cellular imaging; design; fluorescence imaging; instrument; instrumentation; malignant breast neoplasm; meetings; mouse model; novel; novel strategies; public health relevance; reproductive; tool; tumor growth

DESCRIPTION (provided by applicant): This is a shared instrumentation proposal to acquire an IVIS(R) Spectrum imaging system, manufactured by the Xenogen Corporation, recently acquired by Caliper Life Sciences. The IVIS Spectrum is a multimodal bioluminescent and fluorescent imaging system specifically designed for noninvasive imaging of cells and tissues in small animals. Bioluminescence and fluorescence imaging is now widely accepted as a valuable research tool for studying a broad range of biological processes in small animals, including tumor growth, cancer metastasis, bacterial infections, immune responses and inflammation, and regulation of tissue-specific gene expression. Although the University of Toledo Health Science Center has a cadre of nationally recognized NIH-funded investigators working in these areas, the University currently does not have an imaging system designed for use with live animals. Consequently, investigators have had to seek alternative approaches that may be more costly or less effective. By meeting a critical need for small animal fluorescence and bioluminescence imaging technology, the acquisition of the Xenogen IVIS Spectrum system will greatly facilitate progress on seven active NIH-sponsored R01 projects directed by the major users. In addition, access to this shared equipment will extend to minor users and new users who have active grant support or are seeking to develop preliminary data for new grant applications. The equipment will be located in the Division of Laboratory Animal Medicine, to facilitate studies with transgenic and immunocompromised mouse models. The day-to-day scheduling, training, and technical support for the instrument will be handled under the auspices of the Advanced Microscopy and Imaging Center, with administrative supervision from the PI and an Internal Advisory Committee. Institutional support for the instrument will come in the form of space allocation for the imaging lab and a 50% subsidy for the annual service contract. Ongoing projects that will benefit from the Xenogen IVIS Spectrum include: 1) Studies aimed at exploring the therapeutic potential of novel cell death mechanisms in glioblastoma; 2) Studies of the effects of steroid receptor agonists and antagonists on the growth of malignancies of the female reproductive tract; 3) Investigations into the mechanisms that give rise to neuroblastoma; 4) Studies aimed at defining new approaches to induce transplant tolerance; 5) Investigations aimed at understanding the behaviors of specific types of cells involved in immune surveillance against infections in the skin; 6) Studies of the roles of newly discovered signaling molecules in the regulation of breast cancer growth; and 7) Studies aimed at understanding the roles of specific proteins that control insulin action in diabetes. Several new projects focused on breast cancer metastasis, development of drug resistance, fat cell differentiation, and immune response to infection by the organism that causes Lyme disease will also benefit from the availability of the requested imaging system. By accelerating progress on these diverse projects, this shared instrumentation will have a major positive impact on health-related research. PUBLIC HEALTH RELEVANCE: We are requesting funds to acquire a Xenogen IVIS(R) Spectrum system, which is the latest model instrument available for noninvasive bioluminescent and fluorescent imaging of cells and tissues in small animals. This instrument is not currently available at this university. It will be operated as a shared resource to accelerate the health-related research of seven major users who are engaged in NIH-sponsored projects focused on cancer, transplantation, immune response to infection, and diabetes.

描述（由申请人提供）：这是一份共享仪器提案，旨在获得由Xenogen公司生产的IVIS(R)光谱成像系统，该系统最近被Caliper生命科学公司收购。IVIS光谱是一种多模态生物发光和荧光成像系统，专为小动物细胞和组织的无创成像而设计。生物发光和荧光成像现在被广泛认为是一种有价值的研究工具，用于研究小动物的广泛生物过程，包括肿瘤生长，癌症转移，细菌感染，免疫反应和炎症，以及组织特异性基因表达的调节。尽管托莱多大学健康科学中心有一批国家认可的nih资助的研究人员在这些领域工作，但该大学目前还没有设计用于活体动物的成像系统。因此，调查人员不得不寻求可能成本更高或效果更差的替代方法。通过满足对小动物荧光和生物发光成像技术的关键需求，Xenogen IVIS光谱系统的收购将极大地促进由主要用户指导的七个活跃的nih赞助R01项目的进展。此外，使用这种共享设备的机会将扩大到获得积极赠款支持或正在寻求为新的赠款申请开发初步数据的未成年用户和新用户。该设备将位于实验动物医学部，以促进转基因和免疫受损小鼠模型的研究。仪器的日常安排、培训和技术支持将在高级显微镜和成像中心的主持下进行，并由PI和内部咨询委员会进行行政监督。该仪器的机构支持将以成像实验室的空间分配和年度服务合同的50%补贴的形式出现。正在进行的项目将受益于Xenogen IVIS光谱包括：1)旨在探索胶质母细胞瘤新细胞死亡机制的治疗潜力的研究；2)类固醇受体激动剂和拮抗剂对女性生殖道恶性肿瘤生长影响的研究；3)神经母细胞瘤发生机制的研究；4)旨在确定诱导移植耐受新方法的研究；5)旨在了解参与皮肤感染免疫监视的特定类型细胞的行为的研究；6)新发现的信号分子在乳腺癌生长调控中的作用研究；7)旨在了解控制胰岛素作用的特定蛋白质在糖尿病中的作用的研究。几个新项目的重点是乳腺癌转移、耐药性的发展、脂肪细胞分化和莱姆病病原菌感染的免疫反应，这些项目也将受益于所需成像系统的可用性。通过加快这些不同项目的进展，这种共享的工具将对与健康有关的研究产生重大的积极影响。