Phosphorimager Replacement

描述（由申请人提供）：这是一份申请，旨在获得资金，将我们现有的Typhoon 8600可变模式成像仪更换为Typhoon 9410，这是一种具有增强功能的相关仪器。现有的仪器只有五年的历史，但需求量很大，在上一个财政年度进行了约7000次扫描。在过去的几年里，服务电话的数量和相应问题的严重性急剧增加。制造商不再销售8600，因此对该仪器的支持可能有限。在NIH的19赠款下，13名研究人员每天都依靠这种仪器进行研究。由其他机构资助的其他一些调查人员也依赖这一工具。台风的使用几乎完全取消了使用昂贵的X射线胶片来检测放射性核素。然而，X射线胶片仍然用于化学发光应用，特别是Western印迹。8600缺乏使用最灵敏的化学荧光试剂所需的蓝色激光，可用于蛋白质印迹应用;因此，我们被迫几乎完全使用化学发光。9410具有所需的蓝色激光。购买Typhoon 9410将通过提高灵敏度来增强化学荧光能力，确保NIH支持的19个研究项目的不间断进展，并消除与使用X射线胶片进行化学发光应用相关的成本。 公共卫生相关性：美国国立卫生研究院资助的项目将从购买台风9410中受益，重点关注从病毒复制和发病机制到癌症的主题，最终目标是预防，诊断和/或治疗困扰美国和世界各地公民的疾病。

DESCRIPTION (provided by applicant): This is an application to obtain funds to replace our existing Typhoon 8600 variable mode imager with a Typhoon 9410, a related instrument with enhanced capabilities. The existing instrument is only five years old but has been in high demand, ~7000 scans during the past fiscal year. Over the past few years, the number of service calls and the severity of the corresponding problems have increased dramatically. The manufacturer no longer sells the 8600, so support for this instrument may be limited. Thirteen investigators, supported by 19 grants from NIH, rely on this instrument on a daily basis to perform their research. Several other investigators, funded by other agencies, rely on this instrument as well. The use of the typhoon has almost completely eliminated the use of expensive X- ray film for detection of radionuclides. However, X-ray film is still used for chemiluminescence applications, especially Western blotting. The 8600 lacks the blue laser required for use of the most sensitive chemifluorescence reagents available for Western-blotting applications; therefore, we have been forced to use chemiluminescence almost exclusively. The 9410 has the required blue laser. Acquisition of a Typhoon 9410 will enhance chemifluorescence capabilities by increasing sensitivity, ensure uninterrupted progress on the 19 NIH-supported research projects, and eliminate the cost associated with the use of X-ray film for chemiluminescence applications. PUBLIC HEALTH RELEVANCE: The NIH-funded projects that will benefit from the purchase of the Typhoon 9410 focus on topics ranging from virus replication and pathogenesis to cancer with the ultimate goal of preventing, diagnosing and/or treating diseases afflicting citizens of the United States and around the world.