Ion Mobility Time-of-flight Mass Spectrometry System

描述（由申请人提供）：俄勒冈州州立大学（OSU）向NCRR寻求资金，以购买与nanoAcquity UPLC系统耦合的沃茨四极行波离子迁移正交加速飞行时间质谱仪。新仪器将支持NIH资助的炎症，神经退行性和年龄相关疾病以及传染病的研究计划。新设备将支持的七个主要项目和七个次要项目分为三个领域：1）与细胞信号、氧化应激反应和衰老相关的非共价蛋白质相互作用研究; 2）蛋白质组学类型的应用，包括鉴定氧化应激介导的疾病、年龄相关功能障碍和传染病中的翻译后修饰;和3）炎性疾病和癌症中的代谢组学。新仪器将为OSU带来新技术，包括MSE能力、高达8000 Th的前体离子选择和离子迁移率分离。该仪器将由俄勒冈州立大学的质谱设施运行和维护，该设施自1973年以来一直在运行。这个共享的仪器实验室目前由三名经验丰富的教师研究助理组成。该设施的运作费用由机构支助、国家环境卫生科学研究所提供的主要核心中心赠款和用户费支付。设立了一个监督委员会，以协调行政政策和该工具的使用。新设备将是全州努力建立一个俄勒冈州蛋白质组联盟的一部分，其中俄勒冈州的主要研究型大学参与促进先进生物医学科学研究的前沿研究环境中的合作，并为年轻科学家提供最先进仪器的培训机会。公共卫生相关性：所寻求的资金将用于购买先进的质谱系统，该系统将通过支持俄勒冈州州立大学和俄勒冈州其他主要学术机构的传染性，炎症和与年龄有关的疾病的研究计划直接影响公共卫生。新设备将促进学生和博士后研究人员在现代生物分析技术方面的培训，促进在工业和学术界实现职业生涯。

DESCRIPTION (provided by applicant): Oregon State University (OSU) seeks funds from the NCRR to purchase a Waters quadrupole traveling wave ion mobility orthogonal acceleration time-of-flight mass spectrometer coupled to nanoAcquity UPLC system. The new instrument will support NIH-funded research programs in inflammatory, neurodegenerative and age-related disorders as well as infectious diseases. The seven major and seven secondary projects that will be supported by the new equipment fall into three areas: 1) non-covalent protein interaction studies relevant to cell signaling, oxidative stress response and aging; 2) proteomics-type applications including the identification of post- translational modifications in oxidative stress-mediated diseases, age-related dysfunctions and infectious diseases; and 3) metabolomics in inflammatory diseases and cancer. The new instrument will bring new technology to OSU including MSE capability, precursor ion selection up to 8000 Th and ion mobility separation. The instrument will be operated and maintained by OSU's Mass Spectrometry facility, which has been in operation since 1973. This shared instrumentation laboratory is currently staffed by three highly experienced faculty research associates. The operational costs of the facility are covered through institutional support, a major core center grant from the National Institute of Environmental Health Sciences and user fees. An oversight committee has been set up to coordinate administration policy and use of the instrument. The new equipment will be part of a statewide effort to establish an Oregon Proteome Consortium in which the major research universities of Oregon participate to foster collaborations in a cutting-edge research environment for advanced biomedical sciences research and provide training opportunities for young scientists on the most advanced instrumentation available. PUBLIC HEALTH RELEVANCE: The funds sought for will be used toward the purchase of an advanced mass spectrometry system which will directly impact public health by supporting research programs in infectious, inflammatory, and age-related diseases at Oregon State University and other major academic institutions in Oregon. The new equipment will promote training of students and postdoctoral researchers in modern bio-analytical techniques fostering fulfilling careers in industry and academia.