Acquisition of 700 MHz Wide-Bore NMR System for Solid-state NMR

获取用于固态 NMR 的 700 MHz 宽口径 NMR 系统

• 批准号: 7498303
• 负责人: YOSHITAKA ISHII
• 金额: $ 200万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-15 至 2013-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7498303
• 关键词: Amyloid; Amyloid Proteins; Area; Arts; Biocompatible Materials; Biological Factors; Chemistry; Chicago; Collaborations; Commuting; Complement; Development; Disease; Education; Environment; Faculty; Frequencies; Funding; Future; Housing; Illinois; Institutes; Institution; Interdisciplinary Study; Kinetics; Location; Medical; Medical center; Membrane; Membrane Proteins; Methods; Neck; Pharmaceutical Preparations; Proteomics; Public Health; Research; Research Personnel; Site; System; Time; Training; United States National Institutes of Health; Universities; Work; base; biological research; driving force; drug metabolism; graduate student; insight; instrument; member; novel; protein complex; protein misfolding; protein structure; public health relevance; research study; solid state nuclear magnetic resonance; structural biology; tool; trafficking

DESCRIPTION (provided by applicant): Faculty of University of Illinois at Chicago (UIC), in collaboration with the major institutions in the Chicago area such as University of Chicago (UC) and the Argonne National Lab (Argonne) seek NIH funding to acquire a high-field wide-bore NMR system at a 1H frequency of 700 MHz or higher for development of new solid-state NMR (SSNMR) experiments on structural studies of biomolecules. The proposed purchase of an ultra-high- field SSNMR system responds to increasing demands and lack of ultra-high-field SSNMR instruments for biomolecular applications in the US. The proposed system also complements the UIC's existing ultra-high-field systems (800 MHz and 900 MHz) at the Center for Structural Biology in the medical center area because these narrow-bore systems equipped with cryoprobes are incapable of SSNMR experiments. The new spectrometer to be housed in an existing NMR room in the UIC Chemistry Department will be within a commuting distance (~30 min) from other Chicago-area institutions such as Argonne, UC, and Northwestern. Chicago's central location at a traffic hub of the Midwest also makes our site suitable for the unique ultra-high-field wide-bore NMR instrument dedicated for SSNMR in the Midwest. The new instrument will be will be a driving force of new interdisciplinary research and inter-institutional collaborations using SSNMR in this area. The new instrument will serve for our department members (150 graduate students, 20 postdoctral fellows, and 24 faculty) and other researchers (~50) in the Chicago-area institutes including UIC, UC, and, Argonne to perform advanced research in a number of areas including (a) structural biology of amyloid proteins and membrane proteins by SSNMR, (b) protein misfolding kinetics, (c) NMR and SSNMR characterization of novel natural products, organimetallics, and drugs, (d) drug metabolism, (e) membrane association of amyloid, (f) protein structures and dynamics of protein complexes, and (g) analysis of biomembrane bi-layer. In addition to the novel applications of NMR stated above, we will develop new SSNMR methods that would impact broad areas of medical and biological research. In particular, (1) we will develop novel methods to significantly enhance sensitivity in order to attain 10-fold time saving in SSNMR experiments for biomolecules and biomaterials. (2) We will offer new tools for structural analysis of amyloid proteins and membrane proteins, which have been major bottle necks in the current structural proteomics. PUBLIC HEALTH RELEVANCE: The impact of this proposal to the public health is two fold. First, our target includes amyloid proteins and membrane proteins, which are associated with various diseases; structural and functional studies proposed here will provide insights into the mechanisms of the diseases. Second, the state-of-the-art SSNMR instrument easily accessible to researchers in UIC and other Chicago-area institutions will generate excellent opportunities of research-based education on structural analysis of biomolecules by NMR and advanced NMR methods; undergraduate and graduate students will be uniquely trained as future work force in environments relevant to the public health.

描述（申请人提供）：伊利诺伊大学芝加哥分校（UIC），与芝加哥地区的主要机构（如芝加哥大学（UC）和阿贡国家实验室（阿贡））合作，寻求NIH资助，以获得1H频率为700 MHz或更高的高场宽孔NMR系统，用于开发新的固态NMR（SSNMR）生物分子结构研究实验。建议购买超高场SSNMR系统，以应对美国生物分子应用中超高场SSNMR仪器的需求日益增加和缺乏。拟议的系统还补充了UIC现有的超高场系统（800 MHz和900 MHz）在医学中心区域的结构生物学中心，因为这些配备冷冻探针的窄口径系统无法进行SSNMR实验。新的光谱仪将被安置在UIC化学系现有的NMR室中，与芝加哥地区的其他机构（如阿贡，加州大学和西北大学）的通勤距离（约30分钟）。芝加哥位于中西部交通枢纽的中心位置，也使我们的场地适合用于中西部SSNMR的独特超高场宽口径NMR仪器。新仪器将成为该领域使用SSNMR的新跨学科研究和机构间合作的推动力。这台新仪器将为我们系的成员服务（150名研究生，20名博士后研究员和24名教师）和其他研究人员（约50名）在芝加哥地区的研究所，包括UIC，UC和，阿贡进行先进的研究，在一些领域，包括（a）淀粉样蛋白和膜蛋白的结构生物学SSNMR，（B）蛋白质错误折叠动力学，（c）新天然产物、有机金属和药物的NMR和SSNMR表征，（d）药物代谢，（e）淀粉样蛋白的膜缔合，（f）蛋白质结构和蛋白质复合物的动力学，以及（g）生物膜双层的分析。除了上述NMR的新应用外，我们还将开发新的SSNMR方法，这些方法将影响医学和生物学研究的广泛领域。特别是，（1）我们将开发新的方法，以显着提高灵敏度，以实现10倍的时间节省在SSNMR实验的生物分子和生物材料。(2)我们将为淀粉样蛋白和膜蛋白的结构分析提供新的工具，这一直是当前结构蛋白质组学的主要瓶颈。公共卫生相关性：该提案对公共卫生的影响是双重的。首先，我们的目标包括淀粉样蛋白和膜蛋白，它们与各种疾病有关;这里提出的结构和功能研究将为疾病的机制提供见解。其次，UIC和其他芝加哥地区机构的研究人员可以轻松获得最先进的SSNMR仪器，这将为通过NMR和先进的NMR方法进行生物分子结构分析的研究型教育提供极好的机会;本科生和研究生将在与公共卫生相关的环境中接受独特的培训，成为未来的工作力量。

项目成果

Nano-mole scale sequential signal assignment by (1)H-detected protein solid-state NMR.

• DOI: 10.1039/c5cc04618a
• 发表时间: 2015-10-18
• 期刊: Chemical communications (Cambridge, England)
• 作者: Wang S;Parthasarathy S;Xiao Y;Nishiyama Y;Long F;Matsuda I;Endo Y;Nemoto T;Yamauchi K;Asakura T;Takeda M;Terauchi T;Kainosho M;Ishii Y
• 通讯作者: Ishii Y

Evolution of CPMAS under fast magic-angle-spinning at 100 kHz and beyond.

CPMAS 在 100 kHz 及以上快速魔角旋转下的演变。

• DOI: 10.1016/j.ssnmr.2015.10.002
• 发表时间: 2015
• 期刊: Solid state nuclear magnetic resonance
• 影响因子: 3.2
• 作者: Wickramasinghe,Ayesha;Wang,Songlin;Matsuda,Isamu;Nishiyama,Yusuke;Nemoto,Takahiro;Endo,Yuki;Ishii,Yoshitaka
• 通讯作者: Ishii,Yoshitaka

Sensitivity and resolution enhanced solid-state NMR for paramagnetic systems and biomolecules under very fast magic angle spinning.

• DOI: 10.1021/ar4000482
• 发表时间: 2013-09-17
• 期刊: ACCOUNTS OF CHEMICAL RESEARCH
• 影响因子: 18.3
• 作者: Parthasarathy, Sudhakar;Nishiyama, Yusuke;Ishii, Yoshitaka
• 通讯作者: Ishii, Yoshitaka

Progress in proton-detected solid-state NMR (SSNMR): Super-fast 2D SSNMR collection for nano-mole-scale proteins.

• DOI: 10.1016/j.jmr.2017.11.011
• 发表时间: 2018-01
• 期刊: Journal of magnetic resonance (San Diego, Calif. : 1997)
• 作者: Ishii Y;Wickramasinghe A;Matsuda I;Endo Y;Ishii Y;Nishiyama Y;Nemoto T;Kamihara T
• 通讯作者: Kamihara T

Nano-mole scale side-chain signal assignment by 1H-detected protein solid-state NMR by ultra-fast magic-angle spinning and stereo-array isotope labeling.

• DOI: 10.1371/journal.pone.0122714
• 发表时间: 2015
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Wang S;Parthasarathy S;Nishiyama Y;Endo Y;Nemoto T;Yamauchi K;Asakura T;Takeda M;Terauchi T;Kainosho M;Ishii Y
• 通讯作者: Ishii Y