PROTEOMICS AND BIOMINERALIZATION OF DENTIN

牙本质的蛋白质组学和生物矿化

基本信息

批准号：
7892492
负责人：
CHARLES SFEIR
金额：
$ 12.19万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-07-15 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7892492
关键词：
Applications Grants Award Bioethics Biological Calcium Binding Cells Ceramics Characteristics Charge Code Core Facility Crystal Formation Data Dentin Dentin Formation Deposition Diffusion Educational workshop Engineering Environment Equipment Event Exons Extracellular Matrix Proteins Fibroblasts Funding Applicant Gel Genes Goals Grant Growth Habits Hand In Vitro Independent Scientist Award Kinetics Laboratories Learning Mammalian Cell Mass Spectrum Analysis Mediating Methods Minerals Modeling Odontoblasts Osteoblasts Pattern Phosphorylation Phosphorylation Site Physiological Play Post-Translational Protein Processing Preparation Process Proteins Proteomics Regulation Research Research Proposals Role Running Sampling Science Scientist Series Site Specific qualifier value Spectroscopy, Fourier Transform Infrared Structure Surface System Techniques Technology Testing Time Training Universities Writing X ray diffraction analysis X-Ray Diffraction biomineralization calcification career development data management dentin matrix protein 3 design extracellular interfacial macromolecule mass spectrometer mineralization phosphophoryn programs research study secretory protein skills tissue culture

项目摘要

DESCRIPTION (provided by applicant): This Independent Scientist Award (K02) application is designed to support career development in the studies of protein-assisted biomineralization: The role of post-translational modifications of extracellular matrix proteins (ECM) and its impact on mineral formation. This expertise will be applied to the applicant's funded R01 "Dentin Biomineralization" which involves the determination of the difference in phosphorylation patterns and sites of exon 5 of Dentin Matrix Protein 3 using mass spectrometry by analysing recombinantly produced phosphophoryn (PP) as well as purified PP from stably transfected non-mineralizing cells (NIH3T3) and cells that have the capability to mineralize (MC3T3-E1 and MDPC-23). In addition, we will assess how this post- translational modification impacts mineral formation. Career development skills will be applied to collaborative research with Co-PI and consultant. New career development activities in proteomics will include proteomics courses offered at the University of Pittsburgh, intensive workshops and hands on experiments in collaborator and core facility laboratories. This will involve sample preparation, running sample on MALDI-TOF/TOF-MS/MS and sample analysis. Career development in mineral characterization will include tutorials of the necessary equipment (x-ray diffraction, FTIR, SEM, TEM and EDAX), courses in Materials Sciences and Engineering focused on ceramics at Carnegie Mellon University, workshops, sample analysis in collaborator laboratory. The candidate will devote at least 75% of his time to research during the 5-year proposal duration and plans to attend a series of advanced courses, which include responsible conduct in science, career development and grant writing, bioethics, data management, writing and presentation skills. Support of this award will allow the applicant protected time to learn the fundamentals of mass spectrometry and mineral characterization as well as establish a strong research program focused on physiological/pathological biomineralization.

描述（由申请人提供）：该独立科学家奖（K02）的申请旨在支持蛋白质辅助生物矿化研究中的职业发展：细胞外基质蛋白（ECM）翻译后修饰的作用及其对矿物形成的影响。 This expertise will be applied to the applicant's funded R01 "Dentin Biomineralization" which involves the determination of the difference in phosphorylation patterns and sites of exon 5 of Dentin Matrix Protein 3 using mass spectrometry by analysing recombinantly produced phosphophoryn (PP) as well as purified PP from stably transfected non-mineralizing cells (NIH3T3) and cells that have the矿化的能力（MC3T3-E1和MDPC-23）。此外，我们将评估这种翻译后修改如何影响矿物形成。职业发展技能将应用于与Co-Pi和顾问的合作研究。蛋白质组学的新职业发展活动将包括匹兹堡大学提供的蛋白质组学课程，密集的研讨会以及合作者和核心设施实验室中的实验。这将涉及样品制备，在MALDI-TOF/TOF-MS/MS上运行样品以及样品分析。矿物质表征的职业发展将包括必要设备的教程（X射线衍射，FTIR，SEM，TEM和EDAX），材料科学和工程课程，专注于卡内基·梅隆大学的陶瓷，讲习班，合作者实验室的样本分析。候选人将在5年的提案期间将至少75％的时间用于研究，并计划参加一系列高级课程，其中包括科学，职业发展和赠款写作，生物伦理学，数据管理，写作，写作和演示技巧的负责任行为。该奖项的支持将使申请人受保护的时间学习质谱和矿物质表征的基本原理，并建立着针对生理/病理生物矿化的强大研究计划。