HAWAII AIDS CLINICAL RESEARCH PROGRAM/MEDICINE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这项研究的目的是开发一种人脑细胞模型，以评估人类等效剂量的HIV抗逆转录病毒药物对线粒体的影响。 我们的假设是，抗逆转录病毒药物通过降低能量代谢和增加氧化应激导致神经元线粒体功能障碍。 我们建议通过用抗逆转录病毒治疗中使用的人类等效药物剂量治疗原代人类神经元细胞并评估线粒体DNA和RNA水平、ATP产生以及线粒体特异性氧化损伤和抗氧化剂来检验这一假设。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this research is to develop a human brain cell model to evaluate the mitochondrial effects of human equivalent doses of HIV antiretroviral medications. Our hypothesis is that antiretroviral drugs are contributing to mitochondrial dysfunction of neurons by decreasing energy metabolism and increasing oxidative stress. We propose to examine this hypothesis by treating primary human neuronal cells with human equivalent drug doses used in antiretroviral therapy and evaluating mitochondrial DNA and RNA levels, ATP production, and mitochondrial specific oxidative damage and antioxidants.