REGULATION OF GENE EXPRESSION BY ELECTRICAL ACTIVITY IN SPINAL NEURONS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 电活动在调节神经系统的生长和发育中起着重要作用。例如，持续的电活动在神经元存活、轴突生长、神经递质表达和发育中的神经元的电分化的调节中起重要作用。该建议旨在测试在调节基因表达的腰椎运动神经元的电活动的作用。通过表达内向整流钾通道Kir2.1抑制电活动。将使用Affyssin基因芯片鸡基因组阵列评估基因表达的变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Electrical activity plays an important role in regulating the growth and development of the nervous system. For example, ongoing electrical activity plays an important role in the regulation of neuronal survival, axon outgrowth, neurotransmitter expression, and electrical differentiation of developing neurons. This proposal is designed to test the role of electrical activity in regulating gene expression in lumbar motoneurons. Inhibition of electrical activity will be performed by expression of the inward rectifier potassium channel Kir2.1. Changes in gene expression will be assessed using the Affymetrix GeneChip Chicken Genome Array