Molecular Signaling in Cardiac Sarcomeres

心脏肌节的分子信号传导

• 批准号: 7919144
• 负责人: R John Solaro
• 金额: $ 37.61万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7919144
• 关键词: 5' -AMP-activated protein kinase; AMP-activated protein kinase kinase; Admission activity; Aerobic; Affect; Binding; Cardiac; Ceramides; Complement; Complex; Consumption; Coupling; Data; Development; Diagnostic Procedure; Dilated Cardiomyopathy; Disease; Embryo; Energy Supply; Evaluation; Failure; Familial Hypertrophic Cardiomyopathy; Fiber; Financial compensation; Funding; Glycolysis; Heart; Heart Diseases; Heart Hypertrophy; Heart failure; Homeostasis; Hospitals; Hypertrophy; In Situ; Investigation; Link; Metabolic; Metabolic Control; Metabolic Pathway; Metabolism; Microfilaments; Modeling; Modification; Molecular; Mus; Muscle Cells; Mutation; Neonatal; Pathway interactions; Peptides; Phenotype; Phosphorylation; Phosphorylation Site; Phosphotransferases; Post-Translational Protein Processing; Protein Isoforms; Proteins; Proteome; Pump; Reporting; Research; Role; Sarcomeres; Signal Pathway; Signal Transduction; Site; Skin; Sphingolipids; Stress; Testing; Therapeutic; Thin Filament; Troponin; Troponin I; adenylate kinase; base; hemodynamics; link protein; mutant; novel; novel diagnostics; oxidation; p21 activated kinase; programs; protein kinase D; research study; skeletal; sphingosine 1-phosphate; stressor

Our long term objective Project 1 is to understand how signals at the level of the cardiac sarcomere serve to coordinate energy supply and energy consumption. Our aims test the hypothesis that modifications at the level of the sarcomeric proteins serve as significant sites of signal convergence in the progression to heart failure. Our preliminary data indicate a complex coupling, which involves promotion of signaling pathways induced by altered sarcomeric function and that coordinately control energy supply and energy consumption through reciprocol post-translational modifications of sarcomeric proteins. The experiments include investigation of novel findings including: i) differential activation of AMP activated kinase (AMPK) in aerobic conditions in hearts expressing mutant troponin I (cTnl) linked to familial hypertrophic cardiomyopathy (FHC), ii) phosphorylation of cTnl by AMPK; iii) identification of novel cTnl sites of phosphorylation associated with PKCe activation and dilated cardiomyopathy, iii) data predicting metabolically driven sphingolipid signaling to the sarcomeres, and iv) evidence for functionally significant cTnl intra-molecular interactions The specific aims are: Aim #1. To compare the role of AMPK (AMP activated protein kinase) as a signaling mechanism coordinating energy supply and energy consumption in normal hearts and hearts stressed by expression of sarcomeric proteins inducing increases in Ca-sensitivity and FHC. Aim #2. To determine the temporal association of the cardiac phenotype of mice expressing PKCe and demonstrating dilated cardiomyopathy with sarcomeric phosphorylation and whether the phenotype is altered by expression of a non-phosphorylatable mutant Tnl lacking the unique N-terminus. Aim #3. To determine the functional significance of interactions of regions of cTnl with itself and with other thin filament protein sites, potentially significant in coordinating energy demand and supply and modified by AMP kinase (AMPK), protein kinase D (PKD), and PKCe. Approaches to the aims includes studies at the level of the in situ beating heart, isolated myocytes, and skinned fibers with focus on dynamics and evaluation of myofilament Ca-sensitivity and the sarcomere sub-proteome. This project interacts closely with and complements the aims of the other three projects. All three cores strongly support this project. Data generated by the experiments proposed will open a new avenue of research linking metabolic signaling with reciprocal signaling to the sarcomeres, and provide molecular mechanisms of significance in the development of novel diagnostic and therapeutic strategies important in heart failure.

我们的长期目标项目1是了解心脏肌节水平的信号如何协调能量供应和能量消耗。我们的目的是检验这一假设，即在肌节蛋白水平上的修饰是心力衰竭进展中信号会聚的重要位点。我们的初步数据表明，一个复杂的耦合，这涉及到促进信号转导途径诱导的肌节功能改变，协调控制能量供应和能量消耗，通过reciprocol翻译后修饰的肌节蛋白。实验包括新发现的研究，包括：i）在有氧条件下表达与家族性肥厚型心肌病（FHC）相关的突变型肌钙蛋白I（cTnI）的心脏中AMP激活激酶（AMPK）的差异活化，ii）AMPK对cTnI的磷酸化; iii）鉴定与PKCe活化和扩张型心肌病相关的新的cTnI磷酸化位点，iii）预测到肌节的代谢驱动的鞘脂信号传导的数据，和iv）功能上显著的cTnI分子内相互作用的证据。比较AMPK（AMP活化蛋白激酶）作为协调正常心脏和因肌节蛋白表达诱导Ca敏感性和FHC增加而应激的心脏中能量供应和能量消耗的信号机制的作用。目标2。为了确定表达PKCe并证明扩张型心肌病的小鼠的心脏表型与肌节磷酸化的时间关联，以及表型是否通过表达缺乏独特N末端的不可磷酸化的突变Tnl而改变。目标3。为了确定函数 cTnI区域与其自身和与其他细丝蛋白位点相互作用的重要性，在协调能量需求和供应方面可能具有重要意义，并被AMP激酶（AMPK）、蛋白激酶D（PKD）和PKCe修饰。实现这些目标的方法包括在原位跳动心脏、分离的肌细胞和去皮纤维水平上的研究，重点是肌丝Ca敏感性和肌节亚蛋白质组的动力学和评价。该项目与其他三个项目的目标密切互动并相互补充。这三个核心都大力支持这个项目。所提出的实验产生的数据将 开辟了一条新的研究途径，将代谢信号与肌节的相互信号联系起来，并为心力衰竭的新诊断和治疗策略的发展提供了重要的分子机制。