Vevo 2100 Imaging System - High Resolution Ultrasound for Biomicroscopy

描述(申请人提供)：在过去的10年里，对小鼠和大鼠的高分辨率非侵入性成像的需求有了极大的增长，特别是在心血管和心肺领域。转基因动物模型的出现，以及对评估外周血管系统、体内干细胞和基因治疗以及肿瘤生长的兴趣，导致了这种仪器的多种用途的激增。对于一个学术机构来说，提供最先进的超声设备是至关重要的，该设备提供广泛的时间和空间分辨率，足以评估啮齿动物模型的心血管功能、血流和外周循环。这项提案要求为最先进的高分辨率超声生物显微镜设备：视觉超音速Vevo 2100提供资金。该仪器将由伊利诺伊大学芝加哥分校(UIC)研究资源中心管理和操作。该中心的任务是允许新的和现有的研究人员获得通常成本高昂的仪器。该仪器对现有和新招募的研究人员的好处包括：(1)能够对肌肉和肿瘤的血流进行量化，并测量肥厚和适应不良心脏的主动脉和肺动脉反流；(2)以更高的帧速率和更高的分辨率获取数据，同时将这些数据与电生理事件进行门控；(3)准确测量用户定义的心肌区域内的肌肉收缩异常或肌肉收缩的变化。本申请中列出的所有调查人员目前至少有一项R01资金。此外，三名调查人员是最近续签的计划项目拨款的一部分。其中三个主要调查人员也有多份合作出版物。需要使用该仪器的研究项目大致可分为：(1)与肥厚和扩张型心肌病相关的翻译后修饰和突变；(2)基于遗传和干细胞的方法从心肌梗死、高血压和肌节蛋白突变所致的衰竭中拯救心脏；(3)肺血管和右心功能；(4)肌营养不良；(5)骨骼肌与外周缺血相关的氧化应激。

DESCRIPTION (provided by applicant): The need for high resolution non-invasive imaging for mice and rats has seen an enormous increase in the last 10 years, particularly in the cardiovascular and cardiopulmonary field. The advent of transgenic animal models and the interest in assessing the peripheral vasculature, stem cell and gene therapy in vivo, and tumorigenic growth has led to a surge in multiple uses for this instrumentation. It is paramount for an academic institution to provide state-of-the-art ultrasound equipment that offers a wide range of temporal and spatial resolution sufficient for assessing cardiovascular function, blood flow, and peripheral circulation in the rodent model. This proposal requests funding for a state-of-the-art high resolution ultrasound bio microscopy device: the Visual Sonics Vevo 2100. The instrument will be managed and operated by the Research Resources Center at the University of Illinois at Chicago (UIC). The Center's mission is to allow a broad base of new and established investigators access to instrumentation that would ordinarily be cost-prohibitive. The benefits of this instrument to existing and newly recruited investigators are (1) the ability to perform blood flow quantification in muscle and tumors, and measure aortic and pulmonary artery regurgitation in hypertrophic and maladaptive hearts, (2) acquire data at higher frame rates and higher resolution while gating these data to electrophysiological events and (3) accurately measure muscle contractile abnormalities or changes in muscle contraction in a user-defined region of the myocardium. All investigators listed on this application have at least one currently funded R01. In addition, three investigators are part of a recently renewed Program Project Grant. Three of the major investigators also have multiple collaborative publications. Research projects requiring the use of this instrument can be broadly classified as: (1) maladaptive post-translational modifications and mutations linked to hypertrophic and dilated cardiomyopathies, (2) genetic and stem cell based approaches to rescue hearts from failure imposed by myocardial infarction, hypertension and mutations of sarcomeric proteins, (3) pulmonary vascular and right heart function, and (4) muscular dystrophy, and (5) oxidative stress associated with peripheral ischemia in skeletal muscle.