Gene to Structure for $10K (GTS10K)

基因到结构仅需 1 万美元 (GTS10K)

基本信息

  • 批准号:
    7652469
  • 负责人:
  • 金额:
    $ 386.53万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2005
  • 资助国家:
    美国
  • 起止时间:
    2005-07-01 至 2010-06-30
  • 项目状态:
    已结题

项目摘要

Description: The field of structural genomics aims to characterize the structure-function relationships of all proteins by the experimental elucidation of the three dimensional atomic structures of representative members of protein families combined with the accurate computation of models for closely sequence related proteins. The path from gene to structure for eukaryotic proteins is riddled with challenges including the error-prone isolation of gene clones from cDNA libraries, poor yields in protein expression and purification, and time lost between the set-up of crystallization experiments and X-ray diffraction analysis of putative crystals. To address these challenges, we propose the development of integrated instruments, methods and software that will substantially increase the success rate while reducing the time, effort, materials, and cost required for protein structure determination. Among these new developments will be: (1) Rosetta-guided design and gene synthesis of expression optimized DNA sequences encoding proteins that are guided towards more facile purification and improved crystallogenesis; (2) nano-volume microfluidic crystallization devices that embody the major protein crystallization methods allowing extensive crystallization screening of precious protein samples and their complexes; and (3) a tunable in-house MAD X-ray source which will allow in situ room-temperature crystal X-ray diffraction screening and final anomalous dispersion diffraction data collection for rapid structure determination. Starting in year 3, these new technologies will be validated through the planned structure determination and public release of over 550 eukaryotic protein targets belonging to families with high relevance to cancer biology and therapy, including kinases, transcription factors, and metabolic enzymes. The dissemination of the proposed instrumentation, methods and software will allow researchers to pursue protein X-ray crystal structures at or below a total cost of $10,000 per eukaryotic protein structure.
结构基因组学领域旨在通过对蛋白质家族代表成员的三维原子结构的实验阐明,结合对序列密切相关蛋白质模型的精确计算,来表征所有蛋白质的结构-功能关系。真核蛋白从基因到结构的过程充满了挑战,包括从cDNA文库中分离基因克隆容易出错,蛋白质表达和纯化的产量低,以及从结晶实验的建立到假定晶体的x射线衍射分析之间的时间浪费。针对这些挑战,我们提出发展一体化

项目成果

期刊论文数量(22)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A Plug-Based Microfluidic System for Dispensing Lipidic Cubic Phase (LCP) Material Validated by Crystallizing Membrane Proteins in Lipidic Mesophases.
  • DOI:
    10.1007/s10404-009-0512-8
  • 发表时间:
    2010-06
  • 期刊:
  • 影响因子:
    2.8
  • 作者:
    Li, Liang;Fu, Qiang;Kors, Christopher A.;Stewart, Lance;Nollert, Peter;Laible, Philip D.;Ismagilov, Rustem F.
  • 通讯作者:
    Ismagilov, Rustem F.
Gene Composer in a structural genomics environment.
结构基因组学环境中的 Gene Composer。
Discovery of leukotriene A4 hydrolase inhibitors using metabolomics biased fragment crystallography.
  • DOI:
    10.1021/jm900259h
  • 发表时间:
    2009-08-13
  • 期刊:
  • 影响因子:
    7.3
  • 作者:
    Davies DR;Mamat B;Magnusson OT;Christensen J;Haraldsson MH;Mishra R;Pease B;Hansen E;Singh J;Zembower D;Kim H;Kiselyov AS;Burgin AB;Gurney ME;Stewart LJ
  • 通讯作者:
    Stewart LJ
Multiparameter screening on SlipChip used for nanoliter protein crystallization combining free interface diffusion and microbatch methods.
Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer.
  • DOI:
    10.1186/1472-6750-9-37
  • 发表时间:
    2009-04-21
  • 期刊:
  • 影响因子:
    3.5
  • 作者:
    Raymond A;Lovell S;Lorimer D;Walchli J;Mixon M;Wallace E;Thompkins K;Archer K;Burgin A;Stewart L
  • 通讯作者:
    Stewart L
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

LANCE J STEWART其他文献

LANCE J STEWART的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('LANCE J STEWART', 18)}}的其他基金

CRYSTALLOGRAPHIC STUDIES ON HUMAN DNA TOPOISOMERASE I
人类 DNA 拓扑异构酶 I 的晶体学研究
  • 批准号:
    7721789
  • 财政年份:
    2008
  • 资助金额:
    $ 386.53万
  • 项目类别:
CRYSTALLOGRAPHIC STUDIES ON HUMAN DNA TOPOISOMERASE I
人类 DNA 拓扑异构酶 I 的晶体学研究
  • 批准号:
    7597990
  • 财政年份:
    2007
  • 资助金额:
    $ 386.53万
  • 项目类别:
CRYSTALLOGRAPHIC STUDIES ON HUMAN DNA TOPOISOMERASE I
人类 DNA 拓扑异构酶 I 的晶体学研究
  • 批准号:
    7370472
  • 财政年份:
    2006
  • 资助金额:
    $ 386.53万
  • 项目类别:
Gene to Structure for $10K (GTS10K)
基因到结构仅需 1 万美元 (GTS10K)
  • 批准号:
    7256302
  • 财政年份:
    2005
  • 资助金额:
    $ 386.53万
  • 项目类别:
Gene to Structure for $10K (GTS10K)
基因到结构仅需 1 万美元 (GTS10K)
  • 批准号:
    7089035
  • 财政年份:
    2005
  • 资助金额:
    $ 386.53万
  • 项目类别:
Gene to Structure for $10K (GTS10K)
基因到结构仅需 1 万美元 (GTS10K)
  • 批准号:
    6961721
  • 财政年份:
    2005
  • 资助金额:
    $ 386.53万
  • 项目类别:
Gene to Structure for $10K (GTS10K)
基因到结构仅需 1 万美元 (GTS10K)
  • 批准号:
    7459569
  • 财政年份:
    2005
  • 资助金额:
    $ 386.53万
  • 项目类别:
HIGH-THROUGHPUT CRYSTALLIZATION OF PROTEINS
蛋白质的高通量结晶
  • 批准号:
    6143971
  • 财政年份:
    2000
  • 资助金额:
    $ 386.53万
  • 项目类别:
ANTICANCER COMPOUNDS DESIGNED TO POISON TOPOISOMERASE I
旨在毒害拓扑异构酶 I 的抗癌化合物
  • 批准号:
    2903492
  • 财政年份:
    1999
  • 资助金额:
    $ 386.53万
  • 项目类别:
ANTICANCER COMPOUNDS DESIGNED TO POISON TOPOISOMERASE I
旨在毒害拓扑异构酶 I 的抗癌化合物
  • 批准号:
    6319611
  • 财政年份:
    1999
  • 资助金额:
    $ 386.53万
  • 项目类别:

相似海外基金

REU Site: Microbial Biofilm Development, Resistance, & Community Structure
REU 网站:微生物生物膜的发展、耐药性、
  • 批准号:
    2349311
  • 财政年份:
    2025
  • 资助金额:
    $ 386.53万
  • 项目类别:
    Continuing Grant
QUANTUM-TOX - Revolutionizing Computational Toxicology with Electronic Structure Descriptors and Artificial Intelligence
QUANTUM-TOX - 利用电子结构描述符和人工智能彻底改变计算毒理学
  • 批准号:
    10106704
  • 财政年份:
    2024
  • 资助金额:
    $ 386.53万
  • 项目类别:
    EU-Funded
Structure-guided optimisation of light-driven microalgae cell factories
光驱动微藻细胞工厂的结构引导优化
  • 批准号:
    DP240101727
  • 财政年份:
    2024
  • 资助金额:
    $ 386.53万
  • 项目类别:
    Discovery Projects
LSS_BeyondAverage: Probing cosmic large-scale structure beyond the average
LSS_BeyondAverage:探测超出平均水平的宇宙大尺度结构
  • 批准号:
    EP/Y027906/1
  • 财政年份:
    2024
  • 资助金额:
    $ 386.53万
  • 项目类别:
    Research Grant
Statistical Foundations for Detecting Anomalous Structure in Stream Settings (DASS)
检测流设置中的异常结构的统计基础 (DASS)
  • 批准号:
    EP/Z531327/1
  • 财政年份:
    2024
  • 资助金额:
    $ 386.53万
  • 项目类别:
    Research Grant
Structure vs Invariants in Proofs (StrIP)
证明中的结构与不变量 (StrIP)
  • 批准号:
    MR/Y011716/1
  • 财政年份:
    2024
  • 资助金额:
    $ 386.53万
  • 项目类别:
    Fellowship
Structure, Dynamics and Activity of Bacterial Secretosome
细菌分泌体的结构、动力学和活性
  • 批准号:
    BB/Y004531/1
  • 财政年份:
    2024
  • 资助金额:
    $ 386.53万
  • 项目类别:
    Research Grant
Understanding the electronic structure landscape in wide band gap metal halide perovskites
了解宽带隙金属卤化物钙钛矿的电子结构景观
  • 批准号:
    EP/X039285/1
  • 财政年份:
    2024
  • 资助金额:
    $ 386.53万
  • 项目类别:
    Research Grant
Postdoctoral Fellowship: OPP-PRF: Leveraging Community Structure Data and Machine Learning Techniques to Improve Microbial Functional Diversity in an Arctic Ocean Ecosystem Model
博士后奖学金:OPP-PRF:利用群落结构数据和机器学习技术改善北冰洋生态系统模型中的微生物功能多样性
  • 批准号:
    2317681
  • 财政年份:
    2024
  • 资助金额:
    $ 386.53万
  • 项目类别:
    Standard Grant
CAREER: Understanding Processing-Structure-Property Relationships in Co-Axial Wire-Feed, Powder-Feed Laser Directed Energy Deposition
职业:了解同轴送丝、送粉激光定向能量沉积中的加工-结构-性能关系
  • 批准号:
    2338951
  • 财政年份:
    2024
  • 资助金额:
    $ 386.53万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了