HIGH-THROUGHPUT CRYSTALLIZATION OF PROTEINS
HIGH-THROUGHPUT CRYSTALLIZATION OF PROTEINS
批准号:
6143971
负责人:
LANCE J STEWART
金额:
$23.06万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-03-01 至 2001-02-28
关键词:
中文摘要
Emerald BioStructures, Inc.开发一套完整的结晶
英文摘要
Emerald BioStructures, Inc. Is developing an integrated set of crystallization
research tools and software that are designed for high-throughput crystallization
by robots as well as the standard laboratory use by individual researchers with
little or no prior crystallization research experience. These tools provide a
streamlined and systematic approach for identifying crystallization conditions
that yield X-ray diffraction quality protein crystals. In this application, the
investigators propose to develop a systematic and generally applicable method
for the preparation of 10s of milligrams of purified proteins that will fed
directly into their integrated crystallization package, thereby specifically
addressing the rate-limiting aspects of the Structural Genomics Initiative.
We propose to fuse the complementary expertise of Molecular Simulations, Inc.
(Protein structure analysis and target selection using GeneAtlas software),
Invitrogen Corporation (construction and validation of expression-ready Gene
Storm vectors) and, with those of Emerald BioStructure, Inc. (Crystallization
reagents and software, and X-ray structure determination). Our goal is to
automate the production and crystallization of full-length target proteins of
unknown structure or function.
This is made possible by the availability of their novel Crystal Monitor
software which enables high speed collection of crystallization trial data
using speech recognition, which will be programmed to drive custom built
crystallization robots.We will take advantage of Invitrogen's ambitious efforts
to generate expression ready GeneStorm vectors encoding all human open
reading frames (ORF's). Molecular Simulations, Inc., will employ their GeneAtlas
software to identify target human ORFs whose structure determinations are
expected to yield completely novel structural information. Together the companies
will explore the utility of systematic procedures for producing, purifying, and
crystallizing soluble native or refolded proteins expressed in E. Coli or insect cells.
In addition, they propose an innovative procedure for renaturing and crystallizing
proteins in the same vapor diffusion chamber. Crystallization of one or more novel
full-length proteins will establish the feasibility of their approach, thereby
enabling a more fully automated Structural Genomics effort in subsequent phases
of research.
PROPOSED COMMERCIAL APPLICATION:
If successful, this Phase I project would form the basis for an automated system
for protein crystallization. Hence, the proposed research will facilitate the high-
throughput X-ray structure determination of proteins, and help to spawn a new
service industry in macromolecular crystallography.
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会议论文
CRYSTALLOGRAPHIC STUDIES ON HUMAN DNA TOPOISOMERASE I
-
批准号:7721789
-
项目类别:
-
资助金额:$0.02万
-
财政年份:2008
-
负责人:LANCE J STEWART
-
依托单位:
CRYSTALLOGRAPHIC STUDIES ON HUMAN DNA TOPOISOMERASE I
-
批准号:7597990
-
项目类别:
-
资助金额:$0.48万
-
财政年份:2007
-
负责人:LANCE J STEWART
-
依托单位:
CRYSTALLOGRAPHIC STUDIES ON HUMAN DNA TOPOISOMERASE I
-
批准号:7370472
-
项目类别:
-
资助金额:$0.19万
-
财政年份:2006
-
负责人:LANCE J STEWART
-
依托单位:
Gene to Structure for $10K (GTS10K)
-
批准号:7256302
-
项目类别:
-
资助金额:$381.36万
-
财政年份:2005
-
负责人:LANCE J STEWART
-
依托单位:
Gene to Structure for $10K (GTS10K)
-
批准号:7089035
-
项目类别:
-
资助金额:$396.46万
-
财政年份:2005
-
负责人:LANCE J STEWART
-
依托单位:
Gene to Structure for $10K (GTS10K)
-
批准号:6961721
-
项目类别:
-
资助金额:$390.0万
-
财政年份:2005
-
负责人:LANCE J STEWART
-
依托单位:
Gene to Structure for $10K (GTS10K)
-
批准号:7459569
-
项目类别:
-
资助金额:$377.25万
-
财政年份:2005
-
负责人:LANCE J STEWART
-
依托单位:
Gene to Structure for $10K (GTS10K)
-
批准号:7652469
-
项目类别:
-
资助金额:$386.53万
-
财政年份:2005
-
负责人:LANCE J STEWART
-
依托单位:
ANTICANCER COMPOUNDS DESIGNED TO POISON TOPOISOMERASE I
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批准号:2903492
-
项目类别:
-
资助金额:$24.43万
-
财政年份:1999
-
负责人:LANCE J STEWART
-
依托单位:
ANTICANCER COMPOUNDS DESIGNED TO POISON TOPOISOMERASE I
-
批准号:6319611
-
项目类别:
-
资助金额:$29.29万
-
财政年份:1999
-
负责人:LANCE J STEWART
-
依托单位:
HIGH THROUGHPUT XRAY STRUCTURES OF MEMBRANE PROTEINS
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批准号:2867186
-
项目类别:
-
资助金额:$14.05万
-
财政年份:1999
-
负责人:LANCE J STEWART
-
依托单位:
ANTICANCER COMPOUNDS DESIGNED TO POISON TOPOISOMERASE I
-
批准号:6199326
-
项目类别:
-
资助金额:$5.55万
-
财政年份:1999
-
负责人:LANCE J STEWART
-
依托单位:
Anti-Cancer Drug Design Targeting Human Topoisomerase I
-
批准号:6404141
-
项目类别:
-
资助金额:$74.89万
-
财政年份:1998
-
负责人:LANCE J STEWART
-
依托单位:
ANTICANCER DRUG DESIGN TARGETING HUMAN TOPOISOMERASE I
-
批准号:2718909
-
项目类别:
-
资助金额:$9.97万
-
财政年份:1998
-
负责人:LANCE J STEWART
-
依托单位:
Anti-Cancer Drug Design Targeting Human Topoisomerase I
-
批准号:6649262
-
项目类别:
-
资助金额:$69.38万
-
财政年份:1998
-
负责人:LANCE J STEWART
-
依托单位:
海外基金