Role of Acid-Sensing Ion Channels in Pulmonary Vascular Smooth Muscle Store-Opera

酸敏感离子通道在肺血管平滑肌库中的作用-Opera

• 批准号: 7882669
• 负责人: Nikki L Jernigan
• 金额: $ 15.11万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2013-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7882669
• 关键词: ASIC channel; Acids; Acute; Address; Advisory Committees; Agonist; Animals; Binding; Blood Pressure; Blood Vessels; Calcium; Cardiovascular Physiology; Cardiovascular system; Cations; Chronic Obstructive Airway Disease; Chronic lung disease; Confocal Microscopy; Disease; Doctor of Philosophy; Educational process of instructing; Electrophysiology (science); Epithelial; Faculty; Foundations; Funding; Grant; Heart; Heart failure; Human Papillomavirus; Hypoxia; Image; Immunofluorescence Immunologic; Individual; Ion Channel; Journals; Ligands; Lung; Manuscripts; Measures; Mediating; Mediator of activation protein; Membrane; Mentors; Mississippi; Molecular; Neurons; New Mexico; Peer Review; Physiological; Physiology; Postdoctoral Fellow; Protein Family; Proteins; Publishing; Pulmonary Circulation; Pulmonary Vascular Resistance; Pulmonary artery structure; Regulation; Research; Research Proposals; Rest; Reticulum; Reverse Transcriptase Polymerase Chain Reaction; Role; Science; Sleep Apnea Syndromes; Small Interfering RNA; Smooth Muscle Myocytes; Sodium Channel; Stimulus; TRP channel; Techniques; Testing; Thinking; Time; Training; Universities; Vascular Smooth Muscle; Walkers; Work; Workload; Writing; abstracting; career development; experience; graduate student; inhibitor/antagonist; insight; mRNA Expression; member; patch clamp; pressure; programs; receptor; receptor binding; skills; vasoconstriction; voltage

DESCRIPTION (provided by applicant): Dr. Nikki Jernigan earned a PhD in Biomedical Sciences from the University of New Mexico (UNM) and trained as a postdoctoral fellow in the Dept. of Physiology at the University of Mississippi and at UNM. Dr. Jernigan has published 10 first-authored manuscripts in high-quality, peer-reviewed journals. She is a member of the UNM Vascular Physiology Group, which includes six faculty members with expertise in a wide array of technical approaches ranging from whole animal studies to molecular techniques. This group holds a NHLBI-funded Cardiovascular Training Grant, and maintains an excellent record of training both graduate students and postdoctoral fellows. Drs. Walker and Resta will serve as mentors for Dr. Nikki Jernigan's research program and career development. Drs. Kanagy, Prossnitz, and Shuttleworth will serve on Dr. Jernigan's advisory committee. All have strong research programs and considerable experience in mentoring junior faculty. Under the proposed training plan, Dr. Jernigan will build upon her background in cardiovascular physiology and develop new expertise in electrophysiology and calcium imaging. Other facets of Dr. Jernigan's training will be the refinement of her presentation, teaching, writing, and critical thinking skills. The current research proposal focuses on a unique class of ion channels, acid-sensing ion channels (ASIC), and their role in mediating pulmonary vascular smooth muscle (VSM) store-operated calcium entry (SOCE). This work is significant because it will provide new insights into the mechanism of SOCE as a foundation for the regulation of pulmonary vascular resistance, which is altered in disease states such as chronic obstructive pulmonary disease (COPD) and sleep apnea. The long-term objective is to determine the contribution of ASIC-mediated SOCE contributes to elevated pulmonary vascular resistance during chronic lung diseases. Lay Relevance: The pressure in the pulmonary circulation is relatively low compared to the rest of the body. COPD is associated with increased blood pressure in the lung which places an extra workload on the heart and leads to right heart failure. Increases in pulmonary VSM basal calcium and calcium entry contribute to this elevated pressure during COPD. The proposed project will determine the role of a unique class of ion channels, in mediating calcium entry into pulmonary VSM. (End of Abstract)

描述(由申请人提供)： 尼基·杰尼根博士从新墨西哥大学(UNM)获得生物医学博士学位，并在该系接受博士后培训。密西西比大学和密西西比州立大学生理学教授。杰尼根博士在高质量的同行评议期刊上发表了10篇第一作者的手稿。她是UNM血管生理学小组的成员，该小组包括六名教职员工，他们拥有从整体动物研究到分子技术等广泛的技术方法方面的专业知识。这个团队拥有NHLBI资助的心血管培训补助金，并在培训研究生和博士后研究员方面保持着良好的记录。沃克博士和雷斯塔博士将担任尼基·杰尼根博士的研究项目和职业发展的导师。Kanagy、Prossnitz和Shuttleworth博士将在Jernigan博士的顾问委员会任职。它们都有强大的研究项目，并在指导初级教员方面拥有丰富的经验。根据拟议的培训计划，杰尼根博士将在心血管生理学背景的基础上，开发电生理学和钙成像方面的新专业知识。Jernigan博士的培训的其他方面将是她的演讲、教学、写作和批判性思维技能的精炼。目前的研究方案集中在一类独特的离子通道，酸敏感离子通道(ASIC)，以及它们在介导肺血管平滑肌(VSM)储存操作的钙内流(SOCE)中的作用。这项工作具有重要意义，因为它将为SOCE作为调节肺血管阻力的基础的机制提供新的见解，肺血管阻力在慢性阻塞性肺疾病(COPD)和睡眠呼吸暂停等疾病状态下会发生变化。长期目标是确定ASIC介导的SOCE在慢性肺部疾病期间肺血管阻力升高中的作用。 相关性：与身体其他部位相比，肺循环中的压力相对较低。慢性阻塞性肺疾病与肺部血压升高有关，这会给心脏带来额外的工作量，并导致右心衰竭。在COPD期间，肺VSM基础钙和钙进入增加导致这种压力升高。这项拟议的项目将确定一类独特的离子通道在介导钙进入肺VSM中的作用。(摘要结束)