The Cell: An Image Library

描述(由申请人提供)：该项目将建立一个全面的、易于访问的公共资源数据库，其中包括来自各种生物的细胞的图像、视频和动画，包括细胞结构和细胞内功能，以及通过立即部署以及创造和/或保留18个职位来刺激经济。这个数据库的目的是促进对细胞活动的研究，最终目标是改善人类健康。在这个“后基因组”时代，对细胞图像库的关注尤其及时。细胞是组织的基石，在人类胚胎发育过程中经历了动态的重组和形状和运动的变化；这些过程中的异常导致了一些出生缺陷和疾病，如癌症。重要的是，科学家和临床医生充分了解细胞的结构和动态行为，来自各种生物的细胞为我们提供了关于人类细胞生物学的知识。该数据库将包括从当前收藏、出版物和招募中获得的图像。人员将构建网站架构，开发提交方法，审查提交和标签，添加注释，以及宣传和维护网站。具体地说，我们建议：(1)设计和实施图像图书馆所需的电子架构；(2)建立一个经审查的细胞图像数据库(在两年的供资时间内将近15,000张)；(3)评估初步数据库的有效性，并不断纳入基于反馈的修改；(4)寻求对该资源的长期支持以及与其他现有数据库的链接。 与公共健康相关：该提案旨在为各种生物体的细胞开发一个图像库，该图像库将包含高质量的图像、视频和动画，展示细胞的结构和功能。图书馆将是全面的，作为公共资源很容易获取。长期目标是建立一个图像库，作为研究的主要数据。这样一个文库将对健康相关领域的研究产生重大影响，这种影响可与访问基因组序列数据文库带来的变化相媲美。拟议的工作创建了电子基础设施和系统协议，用于获取、评估、注释和上传新图像、视频和动画。这项基础设施发展是该提案的第一个具体目标。在这个“后基因组”时代，对细胞图像库的关注尤其及时。细胞是组织的基石，在人类胚胎发育过程中经历了动态的重组和形状和运动的变化；这些过程中的异常导致了一些出生缺陷和疾病，如癌症。重要的是，科学家和临床医生充分了解细胞的结构和动态行为，来自各种生物的细胞为我们提供了关于人类细胞生物学的知识。在这项提议的两年时间框架内，将有大约15,000张图像进入图像库。随着该项目的继续，预计提交和评价图像的速度将会扩大。通过添加反映用户兴趣并利用新技术和协作的新上传模块，将在图像获取管道中加入长期灵活性。图像的注释将是分层的，从4-5个字的短标签开始，在下一个级别，更多的信息，在更深的级别，图像准备和处理的细节。这种分层的注释满足了图书馆的研究目标，它还将允许教育用户在入门级访问。该提案的第二个具体目标是用带注释的图像来填充图书馆。图像库的新颖性和广度需要来自开发人员、图像提交者、其他来源/资源、注释者和用户的即时和持续的反馈。可以根据收到的反馈，随着内容的增长而对库的用户界面和内容进行修改。调查、使用分析和基准达标率将在整个两年筹资期间提供定期检查点。对贡献者和用户的数据库进行评估是该提案的第三个具体目标。最后，建立、发展和维护图像库成为所有研究人员都可以从中提取信息以回答有关细胞功能的问题的资源，这将远远超过最初的两年资助期。我们将不仅寻求发展额外的资金来源，而且还将发展大规模的合作者，以确保这个图片库的延续和发展。发展资金来源和合作者是这项提案的第四个具体目标。

DESCRIPTION (provided by applicant): This project will establish a comprehensive, easily accessible public resource database of images, videos, and animations of cells from a variety of organisms, including both cell architecture and intracellular functionalities, as well as stimulate the economy through immediate deployment and the creation and/or retention of 18 positions. The purpose of this database is to advance research on cellular activity with the ultimate goal of improving human health. The focus on an Image Library of cells is especially timely in this "post-genomic" era. Cells are the building blocks of tissues and undergo dynamic rearrangements and changes in shape and motility during human embryonic development; abnormalities in these processes underlie some birth defects and diseases such as cancer. It is important that scientists and clinicians fully appreciate the structure and dynamic behavior of cells, and cells from a variety of organisms contribute to our knowledge of human cell biology. The database will include images acquired from current collections, publications, and by recruitment. Personnel will build site architecture, develop submission methods, review submissions and tags, add annotations, and publicize and maintain the site. Specifically, we propose to: (1) Design and implement the electronic architecture needed for the Image Library; (2) Populate a database of reviewed cell images (nearly 15,000 in the two year funding timeframe); (3) Assess the effectiveness of the preliminary database and incorporate feedback-based modifications continuously; (4) Seek long-term support for the resource as well as link with other available databases. PUBLIC HEALTH RELEVANCE: This proposal aims to develop an Image Library for cells from a variety of organisms, a Library that will contain high quality images, videos, and animations demonstrating cellular architecture and functions. The Library will be comprehensive and easily accessible as a public resource. The long-term goal is the construction of a library of images that will serve as primary data for research. Such a library would have a significant impact on research in health-related fields, an impact comparable to the changes wrought by access to a library of genome sequence data. The proposed work creates the electronic infrastructure and a systematic protocol for acquisition, evaluation, annotation, and uploading of new images, videos, and animations. This infrastructure development is the first Specific Aim of the proposal. The focus on an Image Library of cells is especially timely in this "post-genomic" era. Cells are the building blocks of tissues and undergo dynamic rearrangements and changes in shape and motility during human embryonic development; abnormalities in these processes underlie some birth defects and diseases such as cancer. It is important that scientists and clinicians fully appreciate the structure and dynamic behavior of cells, and cells from a variety of organisms contribute to our knowledge of human cell biology. During the two-year timeframe of this proposal, some 15,000 images will populate the Image Library. The rate of submission and evaluation of images is expected to expand as the project continues. Long-term flexibility will be built into the image acquisition pipeline through addition of new upload modules that reflect user interests and take advantage of new technologies and collaborations. Annotation of images will be tiered, starting with short tags of 4-5 words, and at the next level, more information, and at a deeper level, details of image preparation and processing. This layered annotation satisfies the research objective of the library, and it will also allow an educational user access at the entry level. The population of the library with annotated images is the second Specific Aim of the proposal. The novelty and extent of the Image Library requires immediate and continual feedback from developers, image submitters, other sources/resources, annotators, and users. Modifications to the user interface and content of the library can be made as the content grows, based on the feedback received. Surveys, use analysis, and benchmark attainment will provide the periodic checkpoints throughout the two-year funding period. This assessment of the database for contributors and users is the third Specific Aim of the proposal. Finally, the establishment, growth, and maintenance of the Image Library into a resource from which all researchers might extract information to answer questions about the cell's functions will go well beyond the initial two-year funding period. We will seek to develop not only additional funding sources but also large-scale collaborators to ensure continuation and growth of this Image Library. The development of funding sources and collaborators is the fourth Specific Aim of this proposal.