Experimental Mice

9.1.3实验用小鼠共享服务 实验小鼠共享服务(EMSS)是为您体内的所有小鼠提供的一站式共享服务 实验需要。EMSS的目的是为亚利桑那州癌症中心的研究人员提供 (AZCC)使用实验小鼠模型和广泛的小鼠实验技术。这个 EMSS结合了之前的基因工程小鼠“发育”共享服务(GEMSS) 以及之前的EMSS。因此，EMSS从设计和构建基因靶向和 转基因小鼠载体，基因打靶和ES细胞筛选靶向克隆，基因靶向的生产 小鼠，转基因小鼠的生产，转基因和基因敲除创始人的筛选，胚胎 小鼠品系病原体清除和冷冻保存的再衍生直至完成实验 服务于异种移植和宝石模型的方方面面。EMSS的具体目标包括创建 模型、共享和研究人员群体的维护、活体实验设计方面的协助，以及 由精通小鼠实验规程的人员执行小鼠实验。因此， EMSS在小鼠实验内部和之间提供程序的一致性和质量，以确保 实验结果的准确性，对结果的解释的信心，以及 小鼠实验，这对于实验室内和实验室间的比较分析至关重要。由于事实是 MICE是所有转化型癌症工作的典范，既是NIH类型的资助，也是临床进展 在试用过程中，EMSS是一项绝对必要的共享服务。EMSS的好处是为AZCC提供 研究人员具有经济高效的机制来创建不同的老鼠模型并完成 利用训练有素和经验丰富的技术人员进行活体小鼠实验。经济及社会保障计划继续扩展 它的服务非常具有成本效益和效率。

9.1.3 EXPERIMENTAL MOUSE SHARED SERVICE The Experimental Mouse Shared Service (EMSS) is a one-stop shared service for all your in vivo mouse experimentation needs. The purpose of the EMSS is to provide investigators at the Arizona Cancer Center (AZCC) with experimental mouse models and with a wide range of mouse experimentation techniques. The EMSS has combined the previous Genetically Engineered Mouse "Developmental" Shared Service (GEMSS) and the previous EMSS. Therefore, the EMSS begins with the design and construction of gene targeting and transgenic mouse vectors, gene targeting and ES cell screening for targeted Clones, production of genetargeted mice, production of transgenic mice, screening for transgenic and knockout founders, embryo rederivation for pathogen cleanup and cryopreservation of mouse strains through to complete experimentation services in all aspects of xenograft and GEM models. The specific objectives of the EMSS include creation of models, maintenance of shared and investigator colonies, assistance in the design of in vivo experiments, and performance of mouse experiments by personnel with expertise in mouse experimental protocols. Hence, the EMSS provides consistency and quality of procedures within and between mouse experiments that ensures accuracy in experimental results, confidence in interpretation of results, and an inner-consistency between mouse experiments which is essential for intra-lab and inter-lab comparative analyses. Due to the fact that the mouse is a model for all translational cancer work for both NIH-type funding as well as progression to clinical trials, the EMSS is an absolutely essential shared service. The benefits of the EMSS are to provide AZCC investigators with a cost-effective mechanism for the creation of different mouse models and for completing in vivo mouse experiments utilizing highly trained and experienced technicians. The EMSS continues to expand its services in a very cost-effective and efficient manner.