SPATIAL REGULATION OF RAS ACTIVITY DURING CHEMOTAXIS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 RAS是趋化过程中定向感觉的关键调节因子，已被认为是细胞定向罗盘的关键组成部分。我们已经证明，RasGAP(GTPase激活蛋白)控制着RAS活性的时空调节和细胞感知化学吸引物梯度的能力。目前尚不清楚的是，NF1在细胞中的位置。我们建议使用TIRF来定位NF1以响应化学诱导剂的刺激，因为这将有助于我们对信号通路进行建模。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ras is a key regulator of directional sensing during chemotaxis and has been proposed to be a key component of the cell's directional compass. We have demonstrated that the RasGAP (GTPase activation protein) controls the spatiotemporal regulation of Ras activity and the cell's ability to sense the chemoattractant gradient. What is not clear is where NF1 resides in the cell. We propose to use TIRF to localize NF1 in response to chemoattractant stimulation as this will help us model the signaling pathway.