SPATIAL REGULATION OF RAS ACTIVITY DURING CHEMOTAXIS

趋化过程中 RAS 活性的空间调控

• 批准号: 7957645
• 负责人: RICHARD A FIRTEL
• 金额: $ 4.68万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-15 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957645
• 关键词: Cells; Cellular Structures; Chemotactic Factors; Chemotaxis; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Guanosine Triphosphate Phosphohydrolases; Image Analysis; Institution; Modeling; Regulation; Research; Research Personnel; Resources; Signal Pathway; Source; United States National Institutes of Health; directional cell; protein activation; response; spatiotemporal

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ras is a key regulator of directional sensing during chemotaxis and has been proposed to be a key component of the cell's directional compass. We have demonstrated that the RasGAP (GTPase activation protein) controls the spatiotemporal regulation of Ras activity and the cell's ability to sense the chemoattractant gradient. What is not clear is where NF1 resides in the cell. We propose to use TIRF to localize NF1 in response to chemoattractant stimulation as this will help us model the signaling pathway.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 Ras是趋化过程中方向感的关键调节因子，已被认为是细胞方向罗盘的关键组成部分。 我们已经证明，RasGAP（GT3激活蛋白）控制Ras活性的时空调节和细胞的能力，感觉到的趋化梯度。 目前尚不清楚NF 1在细胞中的位置。 我们建议使用TIRF定位NF 1在响应于化学引诱物刺激，因为这将有助于我们模拟信号通路。