SPATIAL REGULATION OF RAS ACTIVITY DURING CHEMOTAXIS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 Ras是趋化过程中方向感的关键调节因子，已被认为是细胞方向罗盘的关键组成部分。 我们已经证明，RasGAP（GT3激活蛋白）控制Ras活性的时空调节和细胞的能力，感觉到的趋化梯度。 目前尚不清楚NF 1在细胞中的位置。 我们建议使用TIRF定位NF 1在响应于化学引诱物刺激，因为这将有助于我们模拟信号通路。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ras is a key regulator of directional sensing during chemotaxis and has been proposed to be a key component of the cell's directional compass. We have demonstrated that the RasGAP (GTPase activation protein) controls the spatiotemporal regulation of Ras activity and the cell's ability to sense the chemoattractant gradient. What is not clear is where NF1 resides in the cell. We propose to use TIRF to localize NF1 in response to chemoattractant stimulation as this will help us model the signaling pathway.