SPATIAL REGULATION OF RAS ACTIVITY DURING CHEMOTAXIS

趋化过程中 RAS 活性的空间调控

• 批准号: 7957645
• 负责人: RICHARD A FIRTEL
• 金额: $ 4.68万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-15 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957645
• 关键词: Cells; Cellular Structures; Chemotactic Factors; Chemotaxis; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Guanosine Triphosphate Phosphohydrolases; Image Analysis; Institution; Modeling; Regulation; Research; Research Personnel; Resources; Signal Pathway; Source; United States National Institutes of Health; directional cell; protein activation; response; spatiotemporal

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ras is a key regulator of directional sensing during chemotaxis and has been proposed to be a key component of the cell's directional compass. We have demonstrated that the RasGAP (GTPase activation protein) controls the spatiotemporal regulation of Ras activity and the cell's ability to sense the chemoattractant gradient. What is not clear is where NF1 resides in the cell. We propose to use TIRF to localize NF1 in response to chemoattractant stimulation as this will help us model the signaling pathway.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 RAS是趋化过程中定向感测的关键调节剂，并被认为是细胞定向指南针的关键组成部分。 我们已经证明了RasGAP（GTPase激活蛋白）控制RAS活性的时空调节和细胞感知化学吸收剂梯度的能力。 不清楚的是NF1居住在细胞中的位置。 我们建议使用TIRF来响应于化学吸引力刺激来定位NF1，因为这将有助于我们对信号传导途径进行建模。