CELLULAR LOCALISATION OF THE TRANSCRIPTIONAL REGULATORS E12 AND E47

转录调节因子 E12 和 E47 的细胞定位

• 批准号: 7957610
• 负责人: CORNELIS MURRE
• 金额: $ 3.12万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-15 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7957610
• 关键词: B-Cell Development; B-Lymphocytes; Biological; Computer Retrieval of Information on Scientific Projects Database; Data; Development; Electron Microscopy; Epitopes; Fluorescence; Funding; Gene Expression; Gene Rearrangement; Grant; Image Analysis; Immunoglobulin Gene Rearrangement; Immunoglobulins; Institution; Label; Lymphocyte; Lymphoid Cell; Mono-S; Research; Research Personnel; Resources; Source; Staging; TCF3 gene; United States National Institutes of Health; cell growth; helix-loop-helix protein E12; insight; transcription factor

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The E2A proteins E12 and E47 are expressed throughout B-cell development to activate B-cell specific gene expression and immunoglobulin gene rearrangement. Here we propose to examine the cellular localization of E12 and E47 during distinct stages of B-cell development utilizing biarsenical-tetracysteine labeling. E12 and E47 are transcriptional regulators that control developmental progression, cellular expansion, survival and gene rearrangement in developing lymphoid cells. How E12 and E47 regulate this diverse set of biological activities is unknown. Additionally it is unclear where E12 and E47 are localized during the distinct stages of lymphocyte development. In this application we propose to tag E12 and E47 utilizing the biarsenical-tetracysteine epitope. We would examine localization by fluorescence as well as EM studies. Ultimately we would like to combine electron microscopy and 3D-FISH to examine the localization of these transcription factors in the immunoglobulin locus. Data obtains from the studies may obtain insights into the mono-allelic activation of immunoglobulin gene rearrangement.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 E2 A蛋白E12和E47在整个B细胞发育过程中表达，以激活B细胞特异性基因表达和免疫球蛋白基因重排。在这里，我们建议检查细胞定位的E12和E47在不同阶段的B细胞的发展利用双砷-四半胱氨酸标记。E12和E47是控制发育中淋巴细胞的发育进程、细胞扩增、存活和基因重排的转录调节因子。E12和E47如何调节这些不同的生物活性尚不清楚。此外，目前还不清楚E12和E47在淋巴细胞发育的不同阶段定位在哪里。在本申请中，我们建议利用双砷-四半胱氨酸表位标记E12和E47。我们将通过荧光和EM研究来检查定位。最后，我们想结合联合收割机电子显微镜和3D-FISH检查这些转录因子在免疫球蛋白基因座的定位。从这些研究中获得的数据可能有助于了解免疫球蛋白基因重排的单等位基因激活。