MOLECULES BOUND TO AMYLOID FIBRILS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 这项研究将使用EELS来成像与阿尔茨海默氏症相关的β-淀粉样纤维结合的碘化小分子。 最近通过专有的生物化学测定法鉴定了几种碘化小分子以高亲和力与淀粉样蛋白原纤维结合。 为了评估这些化合物的放射性标记形式作为阿尔茨海默氏症斑块的体内造影剂的潜在效用，SPECT，这项研究旨在开发一种体外成像方法，以更快地评估这些分子的非放射性标记版本具有以下特征：1）高亲和力，2）高密度，3）标记淀粉样蛋白纤维的高选择性。 如果成功的话，这项研究将产生一种非放射性的方法，可以直观地确认小分子特异性标记组织中淀粉样蛋白沉积物的能力，这将大大加快阿尔茨海默病新诊断剂的发现和开发。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This research will use EELS to image iodinated small molecules bound to Alzheimer¿s related beta-amyloid fibrils. Several iodinated small molecules have recently been identified by a proprietary biochemical assay to bind with high affinity to amyloid fibrils. In order to evaluate the potential utility of radiolabeled versions of these compounds as in vivo contrast agents for Alzheimer¿s plaques by e.g., SPECT, this research seeks to develop an in vitro imaging method to more rapidly assess which non-radiolabeled versions of these molecules have the characteristics of: 1) high affinity, 2) high density, and 3) high selectivity for labeling amyloid fibrils. If successful, this research will result in a non radioactivity-based method to visually confirm the capability of small molecules to specifically label amyloid deposits in tissues, which should dramatically expedite the discovery and development of new diagnostic agents for Alzheimer¿s disease.