Flow Cytometry

流式细胞仪

• 批准号: 7944894
• 负责人: Tucker W LeBien
• 金额: $ 14.14万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-08 至 2014-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7944894
• 关键词: Apoptosis; Arts; Biology; Cancer Center; Cancer Center Support Grant; Cell Count; Cell Cycle; Cell Separation; Cell Size; Cells; Color; Communication; Computer software; Consult; Consultations; Cytometry; Doctor of Philosophy; Educational workshop; Electronic Mail; Event; Flow Cytometry; Goals; Hour; Image; Individual; Journals; Laboratory Technicians; Lasers; Link; Manuals; Methods; Mission; Operative Surgical Procedures; Participant; Phenotype; Policies; Population; Postdoctoral Fellow; Procedures; Protocols documentation; Research; Research Personnel; Resources; Scanning; Science; Scientific Advances and Accomplishments; Scientist; Services; Side; Signal Transduction; Site; Sorting - Cell Movement; Speed; Staining method; Stains; Target Populations; Textbooks; Time; Training; Training Programs; Transplantation; Writing; abstracting; cost; data acquisition; experience; graduate student; handbook; instrument; investigator training; lectures; meetings; online tutorial; programs; release of sequestered calcium ion into cytoplasm; skills; success; web site

9.4.1 ABSTRACT: FLOW CYTOMETRY Director: Tucker LeBien, Ph.D. The mission of the Cancer Center Flow Cytometry Core (hereafter referred to as the Flow Core) is to provide state-of-the-art flow cytometry/cell sorting services, scientific consultation, and technical support to facilitate interaction and advance scientific discovery in a reliable and cost-effective manner. The Flow Core has a 3- laser FACSVantage SE (equipped for high-speed sorting and upgraded with the DiVa option), a 3-laser FACSAria, a 3-laser LSR (I, four FACSCalibur benchtop cytometers and an Amnis ImageStream 100 imaging scanning cytometer. The major services offered are: 1) high-speed multiparameter cell sorting (conducted by a full-time dedicated operator), b) individual investigator training and access to the FACSCaliburs and LSR II, and 3) individual or small group consultation on data acquisition and analysis. The success of the Flow Core is evident by the quality of the science it supports and the broad range of users encompassing the seven research programs.

9.4.1摘要：流式细胞仪 主任：塔克·勒比恩（Tucker Lebien）博士 癌症中心流式细胞仪核心（以下称为流核）的任务是提供 最先进的流式细胞仪/细胞分类服务，科学咨询和技术支持以促进 以可靠且具有成本效益的方式进行互动和进步科学发现。流核有3- 激光FACSVantage SE（配备了高速分类并升级Diva选项） FACSARIA，3射线LSR（I，四个Facscalibur台式细胞仪和AMNIS Imagestream 100 成像扫描细胞仪。提供的主要服务是：1）高速多参数分类 （由全职专用运营商进行），b）个人调查员培训并访问 FACSCALIBURS和LSR II，以及3）有关数据获取和分析的个人或小组咨询。 流动核心的成功是可以通过它支持的科学质量和广泛的 用户涵盖了七个研究计划。