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Flow Cytometry

流式细胞仪

基本信息

批准号：
7944894
负责人：
Tucker W LeBien
金额：
$ 14.14万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-08 至 2014-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7944894
关键词：
Apoptosis Arts Biology Cancer Center Cancer Center Support Grant Cell Count Cell Cycle Cell Separation Cell Size Cells Color Communication Computer software Consult Consultations Cytometry Doctor of Philosophy Educational workshop Electronic Mail Event Flow Cytometry Goals Hour Image Individual Journals Laboratory Technicians Lasers Link Manuals Methods Mission Operative Surgical Procedures Participant Phenotype Policies Population Postdoctoral Fellow Procedures Protocols documentation Research Research Personnel Resources Scanning Science Scientific Advances and Accomplishments Scientist Services Side Signal Transduction Site Sorting - Cell Movement Speed Staining method Stains Target Populations Textbooks Time Training Training Programs Transplantation Writing abstracting cost data acquisition experience graduate student handbook instrument investigator training lectures meetings online tutorial programs release of sequestered calcium ion into cytoplasm skills success web site

项目摘要

9.4.1 ABSTRACT: FLOW CYTOMETRY Director: Tucker LeBien, Ph.D. The mission of the Cancer Center Flow Cytometry Core (hereafter referred to as the Flow Core) is to provide state-of-the-art flow cytometry/cell sorting services, scientific consultation, and technical support to facilitate interaction and advance scientific discovery in a reliable and cost-effective manner. The Flow Core has a 3- laser FACSVantage SE (equipped for high-speed sorting and upgraded with the DiVa option), a 3-laser FACSAria, a 3-laser LSR (I, four FACSCalibur benchtop cytometers and an Amnis ImageStream 100 imaging scanning cytometer. The major services offered are: 1) high-speed multiparameter cell sorting (conducted by a full-time dedicated operator), b) individual investigator training and access to the FACSCaliburs and LSR II, and 3) individual or small group consultation on data acquisition and analysis. The success of the Flow Core is evident by the quality of the science it supports and the broad range of users encompassing the seven research programs.

9.4.1摘要：流式细胞术导演：Tucker LeBien博士癌症中心流式细胞术核心（以下简称流式核心）的使命是提供一流的流式细胞术/细胞分选服务、科学咨询和技术支持，以可靠和具有成本效益方式促进科学发现。流量核心有一个3- 激光FACSVantage SE（配备高速分拣并升级为DiVa选项），3激光 FACSAria，3激光LSR（1，4台FACSCalibur台式细胞仪和Amnis ImageStream 100 成像扫描细胞仪。提供的主要服务有：1）高速多参数细胞分选（由专职操作员进行），B）个体研究者培训和访问 FACSCaliburs和LSR II，以及3）关于数据采集和分析的个人或小组咨询。流动核心的成功是显而易见的，它支持的科学质量和广泛的用户包括七个研究项目。