Cell Models Core

细胞模型核心

基本信息

批准号：
7688324
负责人：
Scott H Randell
金额：
$ 17.76万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7688324
关键词：
Air Alveolar Arts Biological Models Cell Culture Techniques Cell Line Cell model Cells Cellular biology Characteristics Collaborations Communities Cystic Fibrosis Development Disease Dissection Elements Endothelial Cells Environment Epithelial Epithelial Cells Epithelium Fostering Foundations Gastrointestinal tract structure Gene Delivery Gene Expression Gene Proteins Gene Transduction Agent Genes Goals Human Hypoxia Implantable venous access port In Vitro Injury Intestines Investigation Liquid substance Liver Lung Mission Modeling Molecular Motion Mucous Membrane Mus Mutation National Institute of Diabetes and Digestive and Kidney Diseases North Carolina Output Production Recording of previous events Research Research Personnel Resistance Services Small Intestines Stem cells System Testing Tissue Procurements Transplantation Universities airway epithelium body system cell type cost gene therapy improved in vivo in vivo Model meetings new growth novel preclinical study protein expression stem subcutaneous success trafficking uptake vector

项目摘要

Cell models are instrumental for studying basic and applied aspects of gene and molecular therapy for cystic fibrosis (CF). Human airway epithelial cell cultures maintained at an air-liquid interface and displaying mucociliary differentiation similar to the in vivo epithelium faithfully reproduce high resistance to gene therapy vectors characteristic of the native human mucosa. There cultures facilitate understanding vector interaction with target cells and provide a strong platform for pre-clinical studies vital to the success of gene and molecular therapy for CF. A Tissue Procurement and Cell Culture Core was established at the University of North Carolina (UNC) in 1984, under the auspices of the CF Foundation, to provide standardized cell cultures to CF researchers. The Core has supported UNC Gene Therapy for CF projects since 1993 and has increased its output and capabilities to meet growing research demands. The Core routinely makes available cells and media that are unavailable and/or prohibitively expensive if purchased from commercial suppliers. The Core has focused on providing human airway epithelial cell cultures to UNC CF Center investigators. The present application will support continuing essential services and will increase the range of services to the University -wide gene and molecular therapy community. The Core provides human airway epithelial cells in environments more representative of in vivo conditions, supports relevant in vivo models and supplies additional cell types including progenitor cells. To accomplish these goals, we propose the following specific aims; 1) to provide normal and CF human and mouse airway epithelial cells in model systems reproducing important elements of the in vivo airway environment, 2) to cost effectively provide additional lung cell types that are high priority targets for the UNC gene and molecular therapy community, and 3) to cost effectively provide liver and intestine cell types under investigation by the UNC gene and molecular community. Through these functions, and in conjunction with the other Cores in this application, the Cell Models Core will foster collaborations directed at improving vector efficiency to both the airway epithelium and additional cell and organ systems relevant to the research mission of NIDDK.

细胞模型对研究基因和分子疗法的基本和应用方面具有重要作用纤维化（CF）。人类气道上皮细胞培养物保持在空气界面并显示类似于体内上皮忠实再现对基因疗法的高耐药性的粘膜循环分化人类粘膜的矢量特征。那里的文化有助于理解矢量互动与靶细胞一起，为临床前研究提供了一个强大的平台，对基因的成功至关重要 CF的分子疗法。在大学建立了组织采购和细胞培养核心 1984年，在CF基金会的主持下，北卡罗来纳州（UNC）提供标准化的电池 CF研究人员的文化。自1993年以来，核心一直支持CF项目的UNC基因疗法增加了其产出和能力，以满足不断增长的研究需求。核心通常可以提供如果从商业供应商那里购买，则无法获得和/或过高的媒体。核心专注于为UNC CF中心研究人员提供人类气道上皮细胞培养物。本申请将支持持续的基本服务，并将增加服务范围大学 - 全基因和分子疗法社区。核心提供人类气道上皮环境中的细胞更多地代表体内条件，支持相关的体内模型和提供包括祖细胞在内的其他细胞类型。为了实现这些目标，我们建议以下具体目的； 1）在模型系统中提供正常的和小鼠气道上皮细胞再现体内气道环境的重要元素，2）成本有效地提供了其他肺部细胞类型是UNC基因和分子治疗社区的高优先级目标，3）成本有效地提供了UNC基因和分子研究的肝脏和肠细胞类型社区。通过这些功能，并与该应用中的其他核心结合在一起 Model Model Core将促进旨在提高矢量效率到气道上皮的合作以及与NIDDK研究任务相关的其他细胞和器官系统。