Resource for the Development of Biomedical Accelerator Mass Spectrometry (AMS)

描述（由申请人提供）：国家加速器质谱资源（AMS）成立于1999年，旨在使生物医学研究人员能够在探索生物学基本问题时准确量化极低水平的放射性同位素。在这次更新中，我们将通过开发完全集成的HPLC AMS来扩展我们现有的能力，以提高我们的合作者需要的代谢测量能力。我们将开发方法来研究生物化学途径和细胞过程下降到单细胞水平。最后，我们将开发和验证AMS在人类转化研究中的应用方法，这是合作者和服务用户日益增长的需求领域。在拨款期间，我们将继续为研究界提供资源，包括为熟悉辅助医疗系统的研究人员提供服务，培训研究人员的技术和传播资源。为了实现这些目标，我们的具体目标是：通过直接耦合分离仪器，增加AMS的吞吐量。2.） 通过将分子鉴定与定义分离物的定量相结合，增加AMS测量的价值和信息含量，用于非常小的细胞，动物和人类样本的途径分析。3)。在样品材料中使用多种同位素示踪剂提供生物系统的定量。4)。为合作和服务客户提供高通量的精确定量。公共卫生相关性：该中心为填补生物医学研究中超高灵敏度同位素定量的独特生态位提供了新技术。该技术是在不干扰模型系统中的自然代谢的情况下定量终点的理想技术，因此结果是相关的，它允许在人类中进行研究，以确保模型代表人类情况和转化研究。这项技术支持超过60个资助的研究人员。

DESCRIPTION (provided by applicant): The National Resource for Accelerator Mass Spectrometry (AMS) was established in 1999 to enable biomedical researchers to accurately quantify very low levels of radioisotopes while exploring fundamental issues in biology. In this renewal, we will expand our present capabilities by developing a fully integrated HPLC AMS to increase our capabilities for metabolic measurements which our collaborators require. We will develop methods to study biochemical pathways and cellular processes down to the level of the single cell. Finally we will develop and validate methods for the application of AMS in human translational research which is a growing area of demand by collaborators and service users. Throughout the tenure of the grant we will continue to provide a resource to the research community that will include service to investigators familiar with AMS, training of investigators in the technology and dissemination of the Resource. Towards these goals, our specific aims are to: 1.) Increase throughput of AMS through direct coupling to separatory instruments. 2.) Increase the value and information content of AMS measurements by combining molecular identities with quantitation of defined isolates for pathway analysis from very small cellular, animal, and human samples. 3.) Provide quantitation of biological systems using multiple isotopic tracers within sampled materials. 4.) Provide high throughput precision quantitation for collaborative and service clients. PUBLIC HEALTH RELEVANCE: This Center provides new technology for filling the unique niche of ultra-high sensitivity isotope quantitation in biomedical studies The technology is ideal for quantifying endpoints without perturbing the natural metabolism in model systems so that results are relevant and it allows studies to be done in humans to assure that models represent the human situation and for translational research. This technology supports over 60 funded investigators.