Resource for the Development of Biomedical Accelerator Mass Spectrometry (AMS)

描述(由申请人提供)：国家加速器质谱学资源(AMS)成立于1999年，旨在使生物医学研究人员在探索生物学基本问题的同时，准确地量化极低水平的放射性同位素。在这次更新中，我们将通过开发完全集成的高效液相色谱仪AMS来扩展我们现有的能力，以提高我们的代谢测量能力，以满足我们的合作者的要求。我们将开发研究生化途径和细胞过程的方法，深入到单个细胞的水平。最后，我们将开发和验证AMS在人类翻译研究中的应用方法，这是合作者和服务用户日益增长的需求领域。在整个赠款期限内，我们将继续向研究界提供资源，包括向熟悉AMS的调查人员提供服务，对调查人员进行技术培训，以及传播资源。朝着这些目标，我们的具体目标是：1.通过直接连接到分离仪器来增加AMS的吞吐量。2.)通过将分子同一性与从非常小的细胞、动物和人类样本中进行通径分析的已定义分离株的定量相结合，增加AMS测量的价值和信息含量。3.)使用取样材料中的多个同位素示踪剂对生物系统进行定量。4.)为协作型和服务型客户提供高吞吐量的精确量化。公共卫生相关性：该中心为填补生物医学研究中超高灵敏度同位素定量这一独特的利基领域提供了新技术，该技术是在不干扰模型系统中的自然新陈代谢的情况下量化终点的理想技术，因此结果是相关的，它允许在人类身上进行研究，以确保模型代表人类的情况和翻译研究。这项技术支持60多名受资助的调查人员。

DESCRIPTION (provided by applicant): The National Resource for Accelerator Mass Spectrometry (AMS) was established in 1999 to enable biomedical researchers to accurately quantify very low levels of radioisotopes while exploring fundamental issues in biology. In this renewal, we will expand our present capabilities by developing a fully integrated HPLC AMS to increase our capabilities for metabolic measurements which our collaborators require. We will develop methods to study biochemical pathways and cellular processes down to the level of the single cell. Finally we will develop and validate methods for the application of AMS in human translational research which is a growing area of demand by collaborators and service users. Throughout the tenure of the grant we will continue to provide a resource to the research community that will include service to investigators familiar with AMS, training of investigators in the technology and dissemination of the Resource. Towards these goals, our specific aims are to: 1.) Increase throughput of AMS through direct coupling to separatory instruments. 2.) Increase the value and information content of AMS measurements by combining molecular identities with quantitation of defined isolates for pathway analysis from very small cellular, animal, and human samples. 3.) Provide quantitation of biological systems using multiple isotopic tracers within sampled materials. 4.) Provide high throughput precision quantitation for collaborative and service clients. PUBLIC HEALTH RELEVANCE: This Center provides new technology for filling the unique niche of ultra-high sensitivity isotope quantitation in biomedical studies The technology is ideal for quantifying endpoints without perturbing the natural metabolism in model systems so that results are relevant and it allows studies to be done in humans to assure that models represent the human situation and for translational research. This technology supports over 60 funded investigators.