Vascular Pathophysiology and History Core (VPHC)

血管病理生理学和病史核心 (VPHC)

• 批准号: 7662920
• 负责人: Kevin P. Claffey
• 金额: $ 12.35万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-26 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7662920
• 关键词: Acetone; Actinin; Address; Age; Animal Feed; Animal Model; Animals; Antibodies; Antigens; Aorta; Apolipoprotein E; Apoptosis; Area; Arterial Fatty Streak; Atherosclerosis; Biology; Blood Vessels; Bone Marrow; Buffers; CCRL2 gene; Cell Separation; Cells; Cholesterol; Clinic; Color; Computer software; Core Facility; Coupled; Cryoultramicrotomy; Cybernetics; Data; Dehydration; Dermal; Development; Diet; Differentiation Antigens; Dissection; Documentation; Dose; Embryo; Endothelial Cells; Event; Excision; Face; Fatty acid glycerol esters; Freezing; Frozen Sections; Functional disorder; Future; Genetic; Genotype; Goals; Heart; Histology; Human; Image; Image Analysis; Immunohistochemistry; Implant; In Situ; Individual; Inflammatory; Investigation; Kidney; Label; Lectin; Lesion; Lipids; Masks; Methanol; Methods; Microscope; Microscopy; Modeling; Molecular Probes; Mus; PECAM1 gene; Paraffin; Paraffin Embedding; Plant Roots; Population; Preparation; Procedures; Process; Protocols documentation; Quality Control; Reagent; Recording of previous events; Research Personnel; Resolution; Resources; Retinal; Retrieval; Sampling; Services; Signal Pathway; Signal Transduction; Smooth Muscle; Sphingosine-1-Phosphate Receptor; Staining method; Stains; Supporting Cell; Techniques; Testing; Time; Tissue Embedding; Tissue Fixation; Tissue Stains; Tissues; Training; Vascular Diseases; Waxes; Work; alanine aminopeptidase; angiogenesis; aortic arch; capillary bed; digital; experience; human tissue; improved; indexing; laser capture microdissection; macrophage; matrigel; monocyte; oil red O; paraform; programs; research study; response; sample fixation; tissue processing; vascular inflammation

The Histology, Cell and Atheroma Core will provide five main services for investigators within the program project. 1) Tissue Processing and Immuno- and Histological Analysis, 2) Photo-documentation, image analysis and quantification and statistical testing of endpoint data for significance, 3) Mouse Lipidomic Analysis, 4) Primary endothelial cell isolation training and quality control for purity and 5) Isolation and culturing of bone marrow-derived macrophage training and reagents. The core has abundant experience and will coordinate with co-director, Dr. Jonathan Smith, Cleveland Clinic, on the detailed and quantitative analysis of atheroma lesions and murine lipidomics needed for these studies. Advanced histological processing and immunohistochemistry will be available to all projects. A key issue in the program goals has been the utilization of primary cell populations, primarily endothelial and bone marrow derived macrophage. Since different labs and individuals are currently isolating primary cells with variable levels of efficiency and application of quality control assessment of population purity, we have incorporated primary cell isolation and analysis into the core. The support from the core will allow investigators to develop projects and experiments without having technical difficulties with quantitative or cell isolation and analysis protocols is an integral component of the program project, provides expanded opportunities and goals for each project and will continue to be an indispensible resource for future efforts.

组织学，细胞和动脉粥样硬化核心将提供五个主要服务的研究人员在该计划 项目1)组织处理、免疫学和组织学分析，2）照片记录，图像 终点数据的显著性分析、定量和统计学检验，3）小鼠脂质组学 分析，4）原代内皮细胞分离训练和纯度的质量控制，和5）分离和纯化， 骨髓源性巨噬细胞的培养及试剂。核心拥有丰富的经验， 我将与克利夫兰诊所的联合主任乔纳森·史密斯博士协调， 分析这些研究所需的动脉粥样硬化病变和鼠脂质组学。高级组织学 将为所有项目提供处理和免疫组织化学服务。方案目标中的一个关键问题是， 利用原代细胞群，主要是内皮和骨髓来源的巨噬细胞。 由于不同的实验室和个人目前正在以不同的效率水平分离原代细胞， 应用群体纯度的质量控制评估，我们将原代细胞分离和 分析核心。来自核心的支持将使研究人员能够开发项目和实验 没有定量或细胞分离和分析方案的技术困难 计划项目的组成部分，为每个项目提供了扩展的机会和目标，并将 继续是今后努力不可或缺的资源。