Large Animal/Histomorphometry

动物/组织形态计量学核心的目标是提供方案项目赠款的调查人员 (Ppg)为设计和执行涉及动物和动物的研究提供科学、技术和财政支持 心脏组织的组织形态计量学评估。我们最先进的设施容纳并包含所有 进行实验和分析冠脉病变犬标本所需的动物和设备 微栓塞术导致的心力衰竭(HF)以及快速起搏引起的心力衰竭的狗。超过了 在过去的23年里，我们已经建立并完全描述了犬慢性心衰的模型，这是理想的。 适用于PPG中概述的研究。在这个模型中，心力衰竭是由多个连续的 使用微球进行冠状动脉内栓塞会导致存活心肌的丧失。该模型体现了 许多，如果不是全部，人类心力衰竭的后遗症，包括严重的收缩和舒张期左心功能障碍，左心室 扩张性和代偿性肥厚，左心室充盈压力增加，全身血管增加 阻力，心输出量减少，更重要的是，线粒体和特征的异常 左心功能进行性恶化。核心将实现以下目标：1)协调 参与冠脉微栓塞术心力衰竭研究的犬的获取、安置和准备， 2)执行涉及冠状动脉微栓塞术引起的心衰的药理学方案，即假设 负责项目2，3)协调狗和心脏组织的分布 来自冠状动脉微栓塞术诱导的心衰实验和4)进行组织学分析 来自冠状动脉微栓塞术引起的心力衰竭和小鼠研究的组织样本(项目1、2和 4)以及来自PPG项目3所特有的快起搏诱发心力衰竭的狗。服务对象 PPG调查员将由核心领导人(H.N.Sabbah博士)、核心合作伙伴组成的团队提供。 组长(V.G.Sharov医生)、1名助理职员调查员(M.Wang博士)和1名执业兽医 技术专家(Annette Hazel女士)。设施兽医(格雷戈里·海西博士)是一个机构资源 并将免费提供所有必要和必要的兽医支持。

The objective of the Animal/Histomorphometry core is to provide investigators of the program project grant (PPG) scientific, technical and financial support for the design and execution of studies involving animals and histomorphometric assessment of cardiac tissue. Our state-of-the art facility houses and contains all the animals and equipment necessary to execute experiments and analyze specimens from dogs with coronary microembolization-induced heart failure (HF) as well as from dogs with rapid pacing-induced HF. Over the past 23 years, we have developed and fully characterized a canine model of chronic HF which is ideally suited for the studies outlined in the PPG. Heart failure in this model is produced by multiple sequential intracoronary embolizations with microspheres that lead to loss of viable myocardium. The model manifests many, if not all of the sequelae of HF in humans including profound systolic and diastolic LV dysfunction, LV dilation and compensatory hypertrophy, increased LV filling pressures, increased systemic vascular resistance, decreased cardiac output and, importantly, abnormalities of mitochondria and characteristic progressive worsening of LV function. The Core will the perform the following aims: 1) coordinate the acquisition, housing and preparation of dogs involved in the coronary microembolization-induced HF studies, 2) execute pharmacologic protocols involving coronary microembolization-induced HF i.e. assume responsibility for treatment regimens for project 2, 3) coordinate distribution of dogs and cardiac tissues derived from coronary microembolization-induced HF experiments and 4) perform histological analysis of tissue samples derived from coronary-microembolization induced HF and mouse studies (Projects 1, 2 and 4) as well as from dogs with rapid-pacing-induced HF that are specific to project 3 of the PPG. Services to PPG investigators will be provided by a team consisting of the Core Leader (Dr. H.N. Sabbah), the Core Co- Leader (Dr. V.G. Sharov), 1 Assistant Staff Investigator (Dr M. Wang) and 1 Licensed Veterinary Technologists (Ms. Annette Hazel). The facility veterinarian (Dr. Gregory Heisey) is an institutional resource and will provide all the necessary and required veterinary support without charge.