Large Animal/Histomorphometry

大型动物/组织形态计量学

• 批准号: 7750208
• 负责人: HANI N SABBAH
• 金额: $ 28.67万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-14 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7750208
• 关键词: Animals; Arts; Canis familiaris; Cardiac; Cardiac Output; Characteristics; Charge; Chronic; Coronary; Energy Metabolism; Equipment; Financial Support; Functional disorder; Heart failure; Housing; Human; Hypertrophy; Lead; Licensing; Measurement; Microspheres; Mitochondria; Modeling; Mus; Myocardium; Peripheral Resistance; Preparation; Program Research Project Grants; Protocols documentation; Research Personnel; Resources; Services; Specimen; Therapeutic Embolization; Tissue Sample; Tissues; Treatment Protocols; Veterinarians; animal tissue; design; pressure; research study

The objective of the Animal/Histomorphometry core is to provide investigators of the program project grant (PPG) scientific, technical and financial support for the design and execution of studies involving animals and histomorphometric assessment of cardiac tissue. Our state-of-the art facility houses and contains all the animals and equipment necessary to execute experiments and analyze specimens from dogs with coronary microembolization-induced heart failure (HF) as well as from dogs with rapid pacing-induced HF. Over the past 23 years, we have developed and fully characterized a canine model of chronic HF which is ideally suited for the studies outlined in the PPG. Heart failure in this model is produced by multiple sequential intracoronary embolizations with microspheres that lead to loss of viable myocardium. The model manifests many, if not all of the sequelae of HF in humans including profound systolic and diastolic LV dysfunction, LV dilation and compensatory hypertrophy, increased LV filling pressures, increased systemic vascular resistance, decreased cardiac output and, importantly, abnormalities of mitochondria and characteristic progressive worsening of LV function. The Core will the perform the following aims: 1) coordinate the acquisition, housing and preparation of dogs involved in the coronary microembolization-induced HF studies, 2) execute pharmacologic protocols involving coronary microembolization-induced HF i.e. assume responsibility for treatment regimens for project 2, 3) coordinate distribution of dogs and cardiac tissues derived from coronary microembolization-induced HF experiments and 4) perform histological analysis of tissue samples derived from coronary-microembolization induced HF and mouse studies (Projects 1, 2 and 4) as well as from dogs with rapid-pacing-induced HF that are specific to project 3 of the PPG. Services to PPG investigators will be provided by a team consisting of the Core Leader (Dr. H.N. Sabbah), the Core Co- Leader (Dr. V.G. Sharov), 1 Assistant Staff Investigator (Dr M. Wang) and 1 Licensed Veterinary Technologists (Ms. Annette Hazel). The facility veterinarian (Dr. Gregory Heisey) is an institutional resource and will provide all the necessary and required veterinary support without charge.

动物/组织形态计量学核心的目的是提供计划项目赠款的调查员 （PPG）针对涉及动物的研究设计和执行的科学，技术和财政支持以及 心脏组织的组织形态计量学评估。我们最先进的设施房屋，并包含所有 执行实验和分析冠状动脉狗的标本所需的动物和设备 微栓塞引起的心力衰竭（HF）以及快速起搏引起的HF的狗。在 过去23年，我们已经开发并充分表征了慢性HF的犬模型，理想情况下是 适用于PPG中概述的研究。该模型中的心力衰竭是由多个顺序产生的 带有微球导致可行心肌丧失的冠状动脉内栓塞。该模型表现出来 许多人（如果不是全部）HF的后遗症，包括深度收缩和舒张期LV功能障碍，LV 扩张和补偿性肥大，LV填充压力增加，全身血管增加 电阻，心输出量降低，重要的是线粒体和特征性异常 LV功能的进行性恶化。核心将执行以下目的：1）协调 涉及冠状动脉微栓塞引起的HF研究的狗的获取，住房和制备， 2）执行涉及冠状动脉微栓塞诱导的HF的药理方案，即假设 项目2的治疗方案的责任2，3）协调狗和心脏组织的分布 源自冠状动脉微栓塞引起的HF实验，4）进行组织学分析 衍生自冠状动脉微栓塞诱导的HF和小鼠研究的组织样品（项目1、2和 4）以及来自PPG项目3的快速诱导的HF的狗。服务 PPG调查人员将由由核心负责人（H.N. Sabbah博士）组成的团队提供 负责人（V.G. Sharov博士），1名助理参谋长（M. Wang博士）和1名许可的兽医 技术人员（Annette Hazel女士）。设施兽医（Gregory Heisey博士）是一种机构资源 并将在不指控的情况下提供所有必要和必要的兽医支持。