Molecular Biology and Protein Expression

分子生物学和蛋白质表达

• 批准号: 7663370
• 负责人: Rodney M Camire
• 金额: $ 24.2万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7663370
• 关键词: Acids; Area; Awareness; Biology; Chemistry; Coagulation Process; Core Facility; Crystallization; Ensure; Goals; Human Resources; Huntington Disease; Individual; Instruction; Laboratories; Measurement; Molecular Biology; Motivation; Participant; Plasma Proteins; Property; Proteins; Quality Control; Reagent; Recombinant Proteins; Research Personnel; Resources; Services; Training; Vitamin K; Yeasts; expression vector; interest; member; programs; protein expression; protein purification; repository; stable cell line; success

for the large-scale isolation of coagulation proteins from plasma. Core B personnel will provide the necessary purified proteins for crystallization trials conducted by Dr. James Huntington. Finally, the core will provide some quality control services particularly the quantitation of y-carboxyglutamic acid content to verify the integrity of expressed vitamin K-dependent proteins. The Co-Directors will be responsible for the oversight and efficient conduct of these activities and for ensuring equitable service is provided to the projects. RELEVANCE (See instructions): PROJECT/

大型 从血浆中分离凝固蛋白。核心B人员将提供必要的纯化蛋白质 詹姆斯亨廷顿博士进行的结晶试验。最后，核心将提供一些质量控制 特别是γ-羧基谷氨酸含量的定量，以验证表达的完整性， 维生素K依赖蛋白质。联席董事将负责监督和有效开展 这些活动，并确保公平的服务提供给项目。 相关性（参见说明）： 项目/