Benchtop Device for Detection of BoNT in Clinical Samples

我们建议开发用于肉毒杆菌神经毒素(BONT)定量检测的设备 全自动台式仪器的全摩尔灵敏度。我们将采用现有的基于珠子的 Bot Alissa技术，由希望之城的Kalkum实验室开发成整体式微柱 普通液体搬运机器人要使用的格式。我们还将开发一种自动化的、高度敏感的 以及用于集成在机器人中的廉价的基于波导型光学读出系统。由此产生的 期望功能原型系统能够自动处理和分析多个样本 同时且重复(高达每小时96个)。肉毒杆菌的自动校准程序和控制 毒素和非肉毒杆菌毒素蛋白水解酶将包括在内。我们的检测系统将进行测试和验证 复杂的生物基质，如血清、固体器官和食品提取物以及临床样本。

We are proposing to develop devices for the quantitative detection of botulinum neurotoxins (BoNT) at attomolar sensitivity with fully automated bench-top instrumentation. We will adopt the existing bead-based BoNT ALISSA technology, developed by the Kalkum lab at City of Hope, into a monolithic microcolumn format to be utilized by common liquid handling robots. We will also develop an automated, highly sensitive, and inexpensive wave-guide-based optical read-out system for integration in the robots. The resulting functional prototype system is expected to automatically process and analyze multiple samples simultaneously and repeatedly (up to 96 per hour). Automated calibration routines and controls for botulinum toxin and non-botulinum toxin proteases will be included. Our assay system will be tested and validated with complex biological matrices such as serum, solid organ and food extracts as well as clinical samples.