Arthritis Genomics and Bioinformatics

关节炎基因组学和生物信息学

• 批准号: 8128460
• 负责人: CHRISTOPHE O. BENOIST
• 金额: $ 21.88万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8128460
• 关键词: Address; Affect; Algorithms; Animal Model; Animals; Ankle; Architecture; Arthritis; Bioinformatics; Bone Marrow; Cells; Code; Collaborations; Communication; Complex; Computer software; Custom; Data; Data Analyses; Data Set; Data Storage and Retrieval; Databases; Deposition; Development; Evolution; Experimental Models; Gene Expression; Gene Expression Microarray Analysis; Genes; Genetic; Genome; Genomics; Goals; Housing; Human Resources; In Vitro; Individual; Intranet; Joints; Label; Laboratories; Meta-Analysis; Methods; Microarray Analysis; Molecular Biology; Mus; Oligonucleotides; Online Systems; Pathway interactions; Preparation; Principal Investigator; Procedures; Process; RNA; Research; Research Design; Research Personnel; Rest; Review Committee; Sampling; Secure; Software Tools; Specificity; Spottings; Staging; Standardization; Structure; Techniques; Therapeutic; Tissues; Training; Writing; computerized tools; data mining; data sharing; design; experience; flexibility; in vivo; insight; programs; research study; response; tool

The overall goal of the Arthritis Genomics and Bioinformatics Core will be to support investigators in the Program utilizing microarray techniques. The core will provide uniform sample preparation, microarray design, data storage and bioinformatics, thus maximizing the reliability and compatibility of information obtained across the Program. The Core will perform standardized RNA preparation from joint tissues of experimental animals, and the amplification steps to generate labeled probe from small amounts of in vivo or in vitro derived RNA. This should help to generate highly comparable datasets throughout the Program. In addition, the Core will design a study-specific "Arthritis Chip", with spotted oligonucleotides that represent genes that vary during arthritogenesis. These custom chips will provide flexibility, allow a greater throughput than would be economically feasible with whole-genome chips, and again enhance data compatibility between the groups. The Core's bioinformatics personnel, which have acquired significant experience in microarray analysis, will guide, train, and help investigators through data analysis in an open and collaborative manner. The Core will provide access to basic analysis packages, and the ability to write custom software in a data-driven manner, in response to particular experimental situations. In addition, data analysis will benefit from a baseline of microarray data on gene expression during arthritis. A central server for secure data storage will house copies of the data, and Web-based software for data browsing will be applied as a Program-specific intranet and for public posting, as appropriate. In addition, the Core will perform data mining on the assembled data, explorations made possible by the coordinated processing and storage of the data, and by our pre-existing data on the evolution of gene expression in arthritic joints. Cross-experiment analyses will search for gene-gene correlations and clusters throughout a variety of conditions, and will generate functional gene hierarchies. These meta-analyses will enrich each of the individual analyses: defining, for example, the subset of genes that are still induced in spite of a genetic blockade of arthritis will help to pinpoint the cellular or functional level at which the gene is implicated. In addition, the meta-analyses made possible by the combined and coordinated datasets will also provide a unique insight into the "arthritis genome".

关节炎基因组学和生物信息学核心的总体目标是支持研究人员 利用微阵列技术的程序。核心将提供均匀的样品制备、微阵列 设计、数据存储和生物信息学，从而最大限度地提高信息的可靠性和兼容性 通过该计划获得。 Core 将从关节组织中进行标准化 RNA 制备 实验动物，以及从少量体内产生标记探针的扩增步骤 或体外衍生的RNA。这应该有助于在整个计划中生成高度可比的数据集。 此外，核心还将设计一种特定于研究的“关节炎芯片”，其中含有斑点寡核苷酸， 代表在关节炎发生过程中变化的基因。这些定制芯片将提供灵活性，允许更大 吞吐量比全基因组芯片在经济上可行，并再次增强数据 群体之间的兼容性。核心的生物信息学人员，获得了重要的 具有微阵列分析经验，将指导、培训和帮助研究人员以开放的方式进行数据分析 和协作方式。核心将提供对基本分析包的访问，以及编写 以数据驱动的方式定制软件，以响应特定的实验情况。此外， 数据分析将受益于关节炎期间基因表达的微阵列数据基线。一个中央 用于安全数据存储的服务器将保存数据的副本，并且用于数据浏览的基于网络的软件将 酌情用作特定于计划的内联网并用于公开发布。此外，核心将 对组装的数据进行数据挖掘，通过协调处理使探索成为可能 和数据存储，以及我们预先存在的关于关节炎关节基因表达进化的数据。 交叉实验分析将在各种不同的领域中寻找基因与基因的相关性和聚类。 条件，并将产生功能基因层次结构。这些荟萃分析将丰富每个 个体分析：例如，定义尽管存在遗传因素但仍被诱导的基因子集 关节炎的阻断将有助于查明该基因所涉及的细胞或功能水平。在 此外，通过合并和协调的数据集进行荟萃分析也将提供 对“关节炎基因组”的独特见解。