Intra-arterial delivery of skeletal muscle stem cells

骨骼肌干细胞的动脉内输送

• 批准号: 7768143
• 负责人: ZIPORA YABLONKA-REUVENI
• 金额: $ 24.57万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7768143
• 关键词: Acquired Immunodeficiency Syndrome; Address; Adult; Aging; Animals; Area; Asphyxia; Behavioral; Biopsy; Blood Circulation; Cell Separation; Cell Therapy; Cells; Cessation of life; Clinical; Clinical Research; Coculture Techniques; Development; Diabetes Mellitus; Disease; Duchenne muscular dystrophy; Dystroglycan; Dystrophin; Effectiveness; Endothelium; Engraftment; Exploratory/Developmental Grant; Future; Genes; Goals; Guidelines; Hereditary Disease; Injection of therapeutic agent; Intra-Arterial Injections; Intramuscular; Investigation; Knowledge; Lead; Life; Limb structure; Lung; Malignant Neoplasms; Mesenchymal Stem Cells; Methodology; Methods; Modeling; Mus; Muscle; Muscle Cells; Muscle Fibers; Muscle satellite cell; Muscular Dystrophies; Mutation; Myoblasts; Nature; Organ; Outcome; PECAM1 gene; Patients; Pericytes; Proliferating; Property; Protocols documentation; Reporter; Reporting; Research; Research Project Grants; Resolution; Respiratory Muscles; Retinal; Risk; Sarcolemma; Sepsis; Skeletal Muscle; Source; Staging; Stem cells; Striated Muscles; System; Techniques; United States National Institutes of Health; Venous; Wasting Syndrome; Western Blotting; base; clinical practice; combat; congenital muscular dystrophy; femoral artery; in vivo; insight; mdx mouse; muscle form; novel; protein complex; protein expression; public health relevance; regenerative; repaired; satellite cell; skeletal; wasting

DESCRIPTION (provided by applicant): This exploratory project aims to evaluate engraftment and myogenic potential of donor pericytes delivered systemically to host skeletal muscles. The long-term goal is the development of cell-based therapy for combating muscle wasting. The search for means to reduce muscle wasting has been especially intensified in Duchenne muscular dystrophy, a genetic disorder where mutations in the dystrophin gene lead to progressive waste of striated muscles and early lethality. Muscle wasting is also associated with aging and is a hallmark of a number of diseases, including cancer, bacterial sepsis, AIDS and diabetes. Muscle wasting can cause generalized weakness and debilitation and in its extreme, when respiratory muscles are involved, asphyxia and even death. One of the proposed ways to treat muscle wasting disorders is engraftment of donor cells that can contribute to myofiber repair and increase muscle mass. The donor cells would need to be delivered effectively to multiple host muscles, preferentially via circulation. However, ongoing studies have shown that intra-venous delivery of donor cells is problematic because many of the cells are trapped in the lungs (and other filter organs). Thus, at present, intra-arterial cell injection seems to be the favorable means for efficient delivery of donor cells. Satellite cells, bona fide myogenic stem cells and their proliferating progeny are unable to reach target muscles when delivered systemically. Recent studies have pointed to the potential of perivascular cells for cell-based therapy in muscular dystrophy. The characterization of these atypical myogenic sources has been based on their properties following ex vivo expansion. The terms mesoangioblasts (endothelium related CD31+ cells) and pericytes (microvasculature- associated contractile cells that engulf the endothelium) have been used interchangeably without a clear resolution of their actual nature. In all, the functional significance of microvasculature sources of myogenic cells in cell-based muscle therapy has remained a subject of debate. In order to analyze the phenomenon in a reproducible manner, standardized approaches need to be developed for isolating and characterizing these atypical sources of myogenic cells. The specific aims of this application are: 1. Determine whether systemically delivered donor pericytes can target dystrophic muscles and be incorporated into myofibers. 2. Evaluate the potential of the engrafted donor cells to restore expression of proteins essential for muscle integrity. To address these specific aims we will first use dystrophin deficient (mdx) host mice and analyze pericyte effectiveness in restoring dystrophin expression in the sarcolemma. Donor pericytes will be delivered via the femoral artery. The anticipated outcome of the proposed studies will provide valuable insights for cell-based therapies in muscular dystrophies and other muscle wasting conditions. PUBLIC HEALTH RELEVANCE: Muscle wasting is associated with muscular dystrophies and causes generalized weakness and debilitation and in severe cases, leads to early lethality. Muscle wasting is also associated with aging and is a hallmark of a number of diseases including cancer and diabetes. The proposed study will evaluate whether systemically delivered donor cells can reach target muscles and contribute to muscle repair and increased muscle mass. The anticipated results will provide important insights for developing cell-based therapies to combat muscle wasting disorders.

描述（由申请方提供）：该探索性项目旨在评价供体周细胞全身递送至宿主骨骼肌的植入和肌生成潜力。长期目标是开发基于细胞的治疗方法来对抗肌肉萎缩。在杜氏肌营养不良症（一种遗传性疾病，其中肌营养不良蛋白基因的突变导致横纹肌的进行性浪费和早期致死性）中，对减少肌肉消耗的方法的研究尤其得到加强。肌肉萎缩也与衰老有关，是许多疾病的标志，包括癌症、细菌性败血症、艾滋病和糖尿病。肌肉萎缩可导致全身无力和虚弱，在极端情况下，当呼吸肌受累时，会导致窒息甚至死亡。治疗肌肉萎缩症的一种建议方法是移植供体细胞，这有助于肌纤维修复和增加肌肉质量。供体细胞需要有效地输送到多个宿主肌肉，优先通过循环。然而，正在进行的研究表明，静脉内输送供体细胞是有问题的，因为许多细胞被困在肺（和其他过滤器官）中。因此，目前，动脉内细胞注射似乎是有效递送供体细胞的有利手段。卫星细胞、真正的肌源性干细胞及其增殖后代在全身递送时无法到达靶肌肉。最近的研究指出，血管周围细胞的潜力为基础的细胞治疗肌营养不良症。这些非典型肌源性来源的表征是基于它们在体外扩增后的性质。术语中成血管细胞（内皮相关的CD31+细胞）和周细胞（微血管系统相关的收缩细胞，其吞噬内皮）已经互换使用，而没有对其实际性质的明确解析。总之，肌源性细胞的微血管来源在基于细胞的肌肉治疗中的功能意义仍然是一个争论的主题。为了以可重复的方式分析这种现象，需要开发标准化的方法来分离和表征这些非典型来源的肌源性细胞。本申请的具体目的是：1.确定是否全身递送供体周细胞可以靶向营养不良的肌肉并被纳入肌纤维。2.评价移植供体细胞恢复肌肉完整性所必需的蛋白质表达的潜力。为了解决这些具体的目标，我们将首先使用抗肌萎缩蛋白缺陷（mdx）的宿主小鼠和分析周细胞的有效性，在肌膜中恢复抗肌萎缩蛋白的表达。供体周细胞将通过股动脉输送。拟议研究的预期结果将为肌营养不良症和其他肌肉萎缩症的细胞疗法提供有价值的见解。 公共卫生关系： 肌肉萎缩与肌营养不良症有关，导致全身无力和虚弱，严重时会导致早期死亡。肌肉萎缩也与衰老有关，是许多疾病的标志，包括癌症和糖尿病。这项拟议的研究将评估全身递送的供体细胞是否可以到达目标肌肉，并有助于肌肉修复和增加肌肉质量。预期的结果将为开发基于细胞的疗法以对抗肌肉萎缩症提供重要的见解。