A Foundation for the Oral Microbiome and Metagenome
口腔微生物组和宏基因组的基础
基本信息
- 批准号:7796933
- 负责人:
- 金额:$ 18.72万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-05-01 至 2011-03-31
- 项目状态:已结题
- 来源:
- 关键词:Actinobacillus actinomycetemcomitansAmerican Type Culture CollectionAnimal ModelAppointmentBacteriaBibliographyBiochemicalBiologicalBlast CellBrainClinicalCollectionCommunicable DiseasesCommunitiesComputer SimulationDataDatabasesDentalDental cariesDepositionDiseaseDisease AssociationEarEnvironmentFacultyFamilyFoundationsGenbankGene ProteinsGenesGeneticGenomeGenomic LibraryGenomicsGoalsHabitatsHealthHeartHost Defense MechanismHumanHuman MicrobiomeInfectionInfectious Diseases ResearchInstitutesInstitutionInternetInvestigationJointsKnowledgeLaboratoriesLeadLibrariesLifeLinkLiverLungMedicineMetagenomicsMethodsMicrobeMicrobiologyMolecularMolecular AnalysisMouth DiseasesNIH Program AnnouncementsNamesNational Institute of Dental and Craniofacial ResearchNucleic AcidsOnline SystemsOralOral cavityOral healthOrganismPathologicPeriodontal DiseasesPhylogenetic AnalysisPorphyromonas gingivalisPrevalencePrincipal InvestigatorProkaryotic CellsProteinsProteobacteriaPubMedPublicationsQualifyingResearchResearch PersonnelResourcesRibosomal RNASchemeScientistSequence AnalysisSideSiteStreptococcus mutansSurveysSystemic infectionTaxonTaxonomyTreesUnited Statesbasebonecostexperiencegenome sequencinginnovationmembermetagenomic sequencingmicrobialmicrobiomemicroorganismnoveloral bacteriaoral infectionoral microbiomepreventprogramsresearch studyresponsetoolvector
项目摘要
The long-term objective of this research is to characterize the cultivable and currently uncultivated
microorganisms present in the human oral cavity (microbiome) and all of their genes (metagenome) in order
to develop an integrated view of host-microbiome interactions in health and disease. The objectives of this
project are to define the unnamed and uncultured oral taxa and to obtain broad metagenomic sequence
information that is representative of the phylogenetic diversity of these microorganisms. Recent studies
have identified almost 800 species in the oral cavity, of which 35% are named, 11% unnamed but
represented by strains, and 54% are unnamed and known only as 16S rRNA sequence phylotypes. There is
currently no naming scheme for the unnamed 65% of the microbiome, thus it is nearly impossible to link
sequence, phenotypic, prevalence, disease association, and bibliographic information. Aim 1 will make it
possible to link these important types of data by naming the unnamed phylotypes, and establishing a
curated, Internet-accessible Human Oral Microbiome Database for all prokaryotic oral taxa. There are
species from 121 genera in the oral cavity and only minimal genomic information exists for half of these
genera. Aim 2 will obtain partial genome sequences for 105 phylogenetically diverse human oral bacteria.
Genomic libraries for each species will be constructed and 384 clones will be sequenced from each end,
yielding 10%-20% genome coverage for each species. This will add information on 600 genes and proteins
for each species and over 60,000 new gene sequences to the oral metagenome. These phylogenetically
diverse sequences will facilitate attribution of contigs to specific genera or species in other metagenome
projects. Currently, 65% of oral prokaryotes are known only as phylotypes based on 16S rRNA sequences.
Meaningful biological characterization requires having living organisms to manipulate on the bench. Aim 3
will identify and deposit reference strains of approximately 200 unnamed oral phylotypes. Strains from
phenotypically characterized investigator culture collections will be identified by 16S rRNA sequence
analysis and novel reference strains deposited with the American Type Culture Collection. This proposal will
significantly enhance our understanding of the human microbiome and allow eventual understanding of its
interaction with the host in oral health and disease. This proposal is in response to PA-04-131,
Metagenomic Analyses of the Oral Microbiome.
This research will help us to understand the several hundred bacteria that live in the human oral cavity and
their genes. These bacteria can cause tooth decay, periodontal disease, and infections elsewhere in the
body. Knowing more about these many bacteria will allow scientists to develop better methods for treating
and preventing oral diseases.
这项研究的长期目标是描述可栽培和目前未栽培的特征
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Floyd E Dewhirst其他文献
Floyd E Dewhirst的其他文献
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{{ truncateString('Floyd E Dewhirst', 18)}}的其他基金
Cloud-Based Machine Learning and Biomarker Visual Analytics for Salivary Proteomics
基于云的机器学习和唾液蛋白质组生物标志物可视化分析
- 批准号:
10827649 - 财政年份:2023
- 资助金额:
$ 18.72万 - 项目类别:
A Community Knowledgebase to Evaluate Host-Microbial Salivary Proteomics
评估宿主微生物唾液蛋白质组学的社区知识库
- 批准号:
10429378 - 财政年份:2021
- 资助金额:
$ 18.72万 - 项目类别:
A Foundation for the Oral Microbiome and Metagenome
口腔微生物组和宏基因组的基础
- 批准号:
9249523 - 财政年份:2016
- 资助金额:
$ 18.72万 - 项目类别:
A Foundation for the Oral Microbiome and Metagenome
口腔微生物组和宏基因组的基础
- 批准号:
9898161 - 财政年份:2016
- 资助金额:
$ 18.72万 - 项目类别:
A Foundation for the Oral Microbiome and Metagenome
口腔微生物组和宏基因组的基础
- 批准号:
10596650 - 财政年份:2006
- 资助金额:
$ 18.72万 - 项目类别:
A Foundation for the Oral Microbiome and Metagenome
口腔微生物组和宏基因组的基础
- 批准号:
7095759 - 财政年份:2006
- 资助金额:
$ 18.72万 - 项目类别:
A Foundation for the Oral Microbiome and Metagenome
口腔微生物组和宏基因组的基础
- 批准号:
7387409 - 财政年份:2006
- 资助金额:
$ 18.72万 - 项目类别:
A Foundation for the Oral Microbiome and Metagenome
口腔微生物组和宏基因组的基础
- 批准号:
7585799 - 财政年份:2006
- 资助金额:
$ 18.72万 - 项目类别:
A Foundation for the Oral Microbiome and Metagenome
口腔微生物组和宏基因组的基础
- 批准号:
7217362 - 财政年份:2006
- 资助金额:
$ 18.72万 - 项目类别:
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