Mouse Oral Microbiome Database

小鼠口腔微生物组数据库

基本信息

批准号：
9902793
负责人：
Floyd E Dewhirst
金额：
$ 23.6万
依托单位：
ADA FORSYTH INSTITUTE, INC.
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-07-11 至 2020-07-10
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9902793
关键词：
16S ribosomal RNA sequencing Address Affect Animal Model Bacteria Biochemical Biological Biological Models Breeding Canis familiaris Collaborations Collection Communities Culture Techniques Data Databases Dental caries Disease Endodontics Family Felidae Foundations Genetic Genome Genomics Genotype Goals Health Heart Diseases Housing Human In Vitro Infection Investigation Laboratories Length Microbe Modeling Mouth Diseases Mus Names Oral Oral cavity Organism Outcome Parents Pathogenicity Periodontitis Phylogenetic Analysis Play Premature Birth Prevalence Procedures Process Reproducibility Research Research Personnel Resources Ribosomal RNA Role Sampling Scheme Scientist Source Stroke Structure Taxonomy Tooth structure Vendor base culture plates genetic makeup host-microbe interactions human disease human model human pathogen in vivo malignant mouth neoplasm member metagenome microbial microbiome microbiome analysis microbiome components microbiome composition microbiome research mouse model oral bacteria oral microbiome parent grant tissue regeneration tool web-accessible

项目摘要

Project Summary One of the major biological questions being targeted by the oral research community is how microbe-microbe and microbe-host interactions affect health and disease. To achieve real progress on this goal, the following foundation and resources must be in place: 1) A shared ability to identify members of the oral microbiome and to access and analyze microbiome data; 2) Access to isolates of the microbiome for in vitro and in vivo analyses; and 3) Genomic information for all members of the microbiome so that we can understand their genetic makeup, and biochemical and pathogenic potential. The parent grant, “A foundation for the Oral Microbiome and Metagenome” addressed these issues in the following three Aims: 1) Provide the research community with curated taxonomic and genomic information and tools to analyze oral microbiome data; 2) Allow in vivo and in vitro investigation of uncultivated oral taxa by obtaining isolates; and 3) Obtain genome sequences for oral bacterial species currently lacking genomic information. To fully understand microbe-microbe and microbe-host interactions, studies of the human oral microbiome must be supplemented with investigations of defined oral microbiomes in animal model systems, such as mice, so the variables of microbial composition and host genetic background can be controlled (something that is impossible in human only studies). The proposed supplement expands the three aims of the parent grant to include organisms which investigators are likely to encounter in using mouse model systems. Many studies in mice involve putting select human pathogens in the mouths of mice. Unfortunately, such studies cannot document the initial mouse microbiome composition as the majority of bacterial species have not yet been identified and placed in a provisional naming scheme. Completion of this supplement will allow the research community to reliably identify mouse oral bacteria, examine their taxonomic and genomic information in a curated web accessible database, and significantly increase the rigor and reproducibility of oral microbiome research.

项目摘要口头研究界针对的主要生物学问题之一是如何微生物微生物和微生物 - 宿主相互作用会影响健康和疾病。实现真实该目标的进展，以下基础和资源必须实现：1）共享能够识别口服微生物组成员并访问和分析微生物组数据； 2）访问微生物组的分离株进行体外和体内分析； 3）基因组有关微生物组所有成员的信息，以便我们可以理解它们的遗传构成，以及生化和致病潜力。父母赠款，“口头的基础微生物组和元基因组”在以下三个目的中解决了这些问题：1）提供具有策划的分类学和基因组信息和工具的研究界可以分析口服微生物组数据； 2）允许体内和体外调查未经培养的口服分类单元获得分离株； 3）获得目前缺乏口服细菌物种的基因组序列基因组信息。为了充分了解微生物微生物和微生物 - 宿主相互作用，对人口腔微生物组的研究必须补充口腔定义的投资动物模型系统（例如小鼠）中的微生物组，因此微生物组成的变量可以控制寄主遗传背景（仅在人类中是不可能的研究）。拟议的补充剂扩大了父母赠款的三个目标，包括研究人员在使用鼠标模型系统中可能会遇到的生物体。许多在小鼠中进行的研究涉及将某些人类病原体放入小鼠的口中。很遗憾，这样的研究无法记录初始小鼠微生物组组成，这是大多数细菌物种尚未被鉴定并置于临时命名方案中。该补充剂的完成将使研究社区可靠地识别鼠标口头细菌，在可访问的网络中检查其分类学和基因组信息数据库，并显着提高口服微生物组研究的严格性和可重复性。

项目成果

期刊论文数量（26）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Understanding Caries From the Oral Microbiome Perspective.

DOI：
10.1080/19424396.2016.12221036
发表时间：
2016-07
期刊：
Journal of the California Dental Association
影响因子：
0
作者：
A. Tanner;C. Kressirer;L. Faller
通讯作者：
A. Tanner;C. Kressirer;L. Faller

The feline oral microbiome: a provisional 16S rRNA gene based taxonomy with full-length reference sequences.

DOI：
10.1016/j.vetmic.2014.11.019
发表时间：
2015-02-25
期刊：
VETERINARY MICROBIOLOGY
影响因子：
3.3
作者：
Dewhirst, Floyd E.;Klein, Erin A.;Bennett, Marie-Louise;Croft, Julie M.;Harris, Stephen J.;Marshall-Jones, Zoe V.
通讯作者：
Marshall-Jones, Zoe V.

Anaerobic culture to detect periodontal and caries pathogens.