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Mouse Oral Microbiome Database

小鼠口腔微生物组数据库

基本信息

批准号：
9902793
负责人：
Floyd E Dewhirst
金额：
$ 23.6万
依托单位：
ADA FORSYTH INSTITUTE, INC.
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-07-11 至 2020-07-10
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9902793
关键词：
16S ribosomal RNA sequencing Address Affect Animal Model Bacteria Biochemical Biological Biological Models Breeding Canis familiaris Collaborations Collection Communities Culture Techniques Data Databases Dental caries Disease Endodontics Family Felidae Foundations Genetic Genome Genomics Genotype Goals Health Heart Diseases Housing Human In Vitro Infection Investigation Laboratories Length Microbe Modeling Mouth Diseases Mus Names Oral Oral cavity Organism Outcome Parents Pathogenicity Periodontitis Phylogenetic Analysis Play Premature Birth Prevalence Procedures Process Reproducibility Research Research Personnel Resources Ribosomal RNA Role Sampling Scheme Scientist Source Stroke Structure Taxonomy Tooth structure Vendor base culture plates genetic makeup host-microbe interactions human disease human model human pathogen in vivo malignant mouth neoplasm member metagenome microbial microbiome microbiome analysis microbiome components microbiome composition microbiome research mouse model oral bacteria oral microbiome parent grant tissue regeneration tool web-accessible

项目摘要

Project Summary One of the major biological questions being targeted by the oral research community is how microbe-microbe and microbe-host interactions affect health and disease. To achieve real progress on this goal, the following foundation and resources must be in place: 1) A shared ability to identify members of the oral microbiome and to access and analyze microbiome data; 2) Access to isolates of the microbiome for in vitro and in vivo analyses; and 3) Genomic information for all members of the microbiome so that we can understand their genetic makeup, and biochemical and pathogenic potential. The parent grant, “A foundation for the Oral Microbiome and Metagenome” addressed these issues in the following three Aims: 1) Provide the research community with curated taxonomic and genomic information and tools to analyze oral microbiome data; 2) Allow in vivo and in vitro investigation of uncultivated oral taxa by obtaining isolates; and 3) Obtain genome sequences for oral bacterial species currently lacking genomic information. To fully understand microbe-microbe and microbe-host interactions, studies of the human oral microbiome must be supplemented with investigations of defined oral microbiomes in animal model systems, such as mice, so the variables of microbial composition and host genetic background can be controlled (something that is impossible in human only studies). The proposed supplement expands the three aims of the parent grant to include organisms which investigators are likely to encounter in using mouse model systems. Many studies in mice involve putting select human pathogens in the mouths of mice. Unfortunately, such studies cannot document the initial mouse microbiome composition as the majority of bacterial species have not yet been identified and placed in a provisional naming scheme. Completion of this supplement will allow the research community to reliably identify mouse oral bacteria, examine their taxonomic and genomic information in a curated web accessible database, and significantly increase the rigor and reproducibility of oral microbiome research.

项目摘要口腔研究界针对的一个主要生物学问题是，微生物与微生物和微生物与宿主的相互作用影响健康和疾病。达到真实的为了实现这一目标，必须建立以下基础和资源：1）共享识别口腔微生物组成员并访问和分析微生物组数据的能力; 2)获得微生物组的分离物用于体外和体内分析;以及3）基因组微生物组所有成员的信息，以便我们能够了解它们的基因组成，以及生化和致病潜力。家长补助金，“口头基金会 Microbiome and Metagenome”在以下三个目标中解决了这些问题：1）提供研究社区提供精选的分类学和基因组信息和工具进行分析口腔微生物组数据; 2）允许通过以下方法对未培养的口腔分类群进行体内和体外研究获得分离株;和3）获得目前缺乏的口腔细菌物种的基因组序列基因组信息为了充分了解微生物与微生物和微生物与宿主的相互作用，对人类口腔微生物组的研究必须补充对定义的口腔微生物组的研究。微生物组在动物模型系统，如小鼠，所以微生物组成的变量宿主的遗传背景可以被控制（这在人类中是不可能的研究）。拟议的补助金扩大了父母补助金的三个目标，研究人员在使用小鼠模型系统时可能遇到的微生物。许多在老鼠身上进行的研究包括将选定的人类病原体放入老鼠的口中。不幸的是，这些研究不能记录最初的小鼠微生物组组成，细菌种类尚未确定并列入临时命名方案。完成这一补充将使研究界能够可靠地确定小鼠口服细菌，检查他们的分类和基因组信息在一个策划的网络访问数据库，并显着提高口腔微生物组研究的严谨性和可重复性。

项目成果

期刊论文数量（26）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Understanding Caries From the Oral Microbiome Perspective.

DOI：
10.1080/19424396.2016.12221036
发表时间：
2016-07
期刊：
Journal of the California Dental Association
影响因子：
0
作者：
A. Tanner;C. Kressirer;L. Faller
通讯作者：
A. Tanner;C. Kressirer;L. Faller

The feline oral microbiome: a provisional 16S rRNA gene based taxonomy with full-length reference sequences.

DOI：
10.1016/j.vetmic.2014.11.019
发表时间：
2015-02-25
期刊：
VETERINARY MICROBIOLOGY
影响因子：
3.3
作者：
Dewhirst, Floyd E.;Klein, Erin A.;Bennett, Marie-Louise;Croft, Julie M.;Harris, Stephen J.;Marshall-Jones, Zoe V.
通讯作者：
Marshall-Jones, Zoe V.

Cultivation strategies for growth of uncultivated bacteria.