AUTOMATED DETECTION OF REGIONS OF INTEREST IN MEMBRANE TRANSPORTERS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 以前，NIH/GM资助的膜转运体药物遗传学(PMT)研究项目限制了膜转运体到外显子和围绕这些外显子的短侧翼序列中单核苷酸多态(SNP)的发现区域，导致几乎没有利用其他基因组项目提供的信息。然而，通过使用目标外显子周围其他生物的保守序列区域，可以获得对可能发生功能重要的SNP的区域的更多洞察力。将这些信息与重复掩蔽和图形成像相结合，可以快速视觉检测新的SNP搜索空间，并有很好的成功PCR扩增的前景。我们现在正在扩展之前的一项初步研究，通过自动检索和处理350个感兴趣的膜转运体的信息来探索这一想法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Previously, the NIH/GM-funded Pharmacogenetics of Membrane Transporters (PMT) research project restricted areas of single nucleotide polymorphism (SNP) discovery in membrane transporters to exons and short flanking sequences surrounding these exons, resulting in little leveraging of information available from other genome projects. However, by using conserved regions of sequence from other organisms surrounding the exon of interest additional insight can be gained into areas where functionally important SNPs may occur. Combining this information with repeat masking and graphical imaging allows for rapid visual detection of new SNP search spaces with a good prospect of successful PCR amplification. We are now extending a previous pilot study exploring this idea by automating the retrieval and processing of this information for 350 membrane transporters of interest.