Photoacoustic imaging of functional domains on primary motor cortex of monkeys

猴子初级运动皮层功能域的光声成像

• 批准号: 7774637
• 负责人: Xinmai Yang
• 金额: $ 25.16万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7774637
• 关键词: Abbreviations; Animals; Area; Auditory; Blood; Brain; Brain imaging; Cerebrovascular Circulation; Core-Binding Factor; Craniotomy; DAQ; Detection; Development; Dura Mater; Environment; Extinction (Psychology); Forelimb; Functional Imaging; Functional Magnetic Resonance Imaging; Goals; Hand; Hemoglobin; Human; Image; Imagery; Imaging Techniques; Imaging technology; Lasers; Light; Macaca mulatta; Magnetic Resonance Imaging; Measures; Methods; Microscopy; Monitor; Monkeys; Motor Cortex; Movement; Noise; Optical Coherence Tomography; Optics; Parkinson Disease; Penetration; Physiologic pulse; Positron-Emission Tomography; Precentral gyrus; Primates; Procedures; Process; Property; Qualifying; Research; Resolution; Sampling; Signal Transduction; Solutions; Spin Labels; Stress; Stroke; Structure; Surface; System; Techniques; Temperature; Testing; Time; Tissue Sample; Tissues; Ultrasonic Transducer; Ultrasonics; Ultrasonography; Variant; Visual; absorption; attenuation; blood oxygen level dependent; cranium; data acquisition; diffuse optical tomography; imaging modality; irradiation; light scattering; nonhuman primate; optical imaging; public health relevance; relating to nervous system; soft tissue; somatosensory; success; tool

DESCRIPTION (provided by applicant): Functional detection in primate brains has particular advantages because of the similarity between non-human primate brain and human brain and the potential for relevance to a wide range of conditions such as stroke and Parkinson's disease. In the proposed research, we use photoacoustic imaging (PAI) to detect functional changes at primary motor cortex of rhesus monkeys. In a rhesus monkey, primary motor cortex is buried in the precentral gyrus, which extends down 8-9 mm below the surface. Therefore, the advantage of PAI for imaging deeper structures is particularly beneficial for this application. In summary, the advantages of utilizing PAI for the detection of primate brain function include: (1) durotomy is not required, therefore, it is a minimal-invasive process; (2) detecting optical absorption properties, which is intrinsically sensitive and provide high contrast; (3) imaging buried cortical structures; (4) high-resolution of ~100 5m; (5) potentially PAI can be performed in real-time. The long term goal of this research is to develop PAI as a qualified tool for high-resolution functional brain imaging in primate research. The objective of current research is to test the feasibility of PAI for detection of primate brain activation using a minimal-invasive procedure. The establishment of a minimal-invasive procedure for research is important because it reduces the stress on the animal while studies can be carried out in a friendly environment to produce highly reliable results. The underlying hypothesis of this proposal is that PAI can reliably detect primary motor cortex activation associated with forelimb movement in rhesus macaques through the dura matter, and provide depth-resolved functional information. The specific aims of this study include (1) to construct a PAI system for monkey brain cortex imaging; (2) to produce both structural and functional images on monkey brain cortex; (3) to compare the sensitivity and resolution of PAI with other established techniques. PUBLIC HEALTH RELEVANCE: In this project, we propose to test the feasibility of photoacoustic imaging technique for brain function imaging in non-human primates. With photoacoustic imaging, buried cortical structures might be imaged with optical contrast at ultrasound resolution. The success of this project will potentially contribute to studies involving stroke and Parkinson's disease in non- human primates.

描述（由申请人提供）：由于非人类灵长类动物大脑和人类大脑之间的相似性以及与中风和帕金森氏病等多种疾病的潜在相关性，灵长类动物大脑的功能检测具有特殊的优势。 在拟议的研究中，我们使用光声成像（PAI）来检测恒河猴初级运动皮层的功能变化。 在恒河猴中，初级运动皮层埋藏在中央前回中，中央前回向下延伸至表面以下 8-9 毫米。 因此，PAI 对更深结构成像的优势对于该应用特别有利。 综上所述，利用PAI检测灵长类脑功能的优点包括：（1）不需要硬脑膜切开术，因此是一种微创过程； (2) 检测光学吸收特性，本质上灵敏且提供高对比度； (3) 埋藏皮质结构成像； (4) 高分辨率~100 5m； (5)潜在的PAI可以实时执行。 这项研究的长期目标是将 PAI 开发为灵长类动物研究中高分辨率功能性脑成像的合格工具。 当前研究的目的是测试 PAI 使用微创程序检测灵长类动物大脑激活的可行性。 建立微创研究程序非常重要，因为它可以减轻动物的压力，同时研究可以在友好的环境中进行，以产生高度可靠的结果。 该提议的基本假设是，PAI 可以通过硬脑膜可靠地检测与恒河猴前肢运动相关的初级运动皮层激活，并提供深度分辨的功能信息。 本研究的具体目标包括（1）构建猴脑皮层成像的PAI系统； (2) 生成猴脑皮层的结构和功能图像； (3) 将 PAI 的灵敏度和分辨率与其他已建立的技术进行比较。 公共健康相关性：在这个项目中，我们建议测试光声成像技术在非人类灵长类动物中进行脑功能成像的可行性。 通过光声成像，埋藏的皮质结构可以在超声分辨率下通过光学对比度进行成像。 该项目的成功将可能有助于非人类灵长类动物中风和帕金森病的研究。