Photoacoustic imaging of functional domains on primary motor cortex of monkeys

描述（由申请人提供）：灵长类动物脑中的功能检测具有特别的优点，因为非人灵长类动物脑和人脑之间的相似性以及与广泛的病症如中风和帕金森病相关的潜力。 在本研究中，我们利用光声成像技术（派）检测恒河猴初级运动皮层的功能变化。 在恒河猴中，初级运动皮层被埋在中央前回中，中央前回向下延伸到表面以下8-9 mm。 因此，派用于成像较深结构的优点对于该应用特别有益。 总之，利用派检测灵长类动物脑功能的优点包括：（1）不需要切开硬脑膜，因此它是一个微创的过程;（2）检测光吸收特性，这是固有的敏感性和提供高对比度;（3）成像埋藏的皮质结构;（4）高分辨率~100 5 m;（5）潜在地派可以实时执行。 本研究的长期目标是开发派作为灵长类动物研究中高分辨率脑功能成像的合格工具。 目前研究的目的是测试派用于使用微创程序检测灵长类动物脑激活的可行性。 建立微创研究程序非常重要，因为它减少了对动物的压力，同时可以在友好的环境中进行研究，以产生高度可靠的结果。 该建议的基本假设是派可以通过硬脑膜可靠地检测与恒河猴前肢运动相关的初级运动皮层激活，并提供深度分辨的功能信息。 本研究的具体目的包括：（1）构建一个用于猴脑皮层成像的派系统;（2）生成猴脑皮层的结构和功能图像;（3）比较派与其他已建立的技术的灵敏度和分辨率。 公共卫生相关性：在本计画中，我们提出以非人类灵长类动物为研究对象，探讨光声成像技术应用于脑功能成像的可行性。 利用光声成像，可以在超声分辨率下利用光学对比度对埋藏的皮质结构进行成像。 该项目的成功将有助于非人类灵长类动物中风和帕金森病的研究。

DESCRIPTION (provided by applicant): Functional detection in primate brains has particular advantages because of the similarity between non-human primate brain and human brain and the potential for relevance to a wide range of conditions such as stroke and Parkinson's disease. In the proposed research, we use photoacoustic imaging (PAI) to detect functional changes at primary motor cortex of rhesus monkeys. In a rhesus monkey, primary motor cortex is buried in the precentral gyrus, which extends down 8-9 mm below the surface. Therefore, the advantage of PAI for imaging deeper structures is particularly beneficial for this application. In summary, the advantages of utilizing PAI for the detection of primate brain function include: (1) durotomy is not required, therefore, it is a minimal-invasive process; (2) detecting optical absorption properties, which is intrinsically sensitive and provide high contrast; (3) imaging buried cortical structures; (4) high-resolution of ~100 5m; (5) potentially PAI can be performed in real-time. The long term goal of this research is to develop PAI as a qualified tool for high-resolution functional brain imaging in primate research. The objective of current research is to test the feasibility of PAI for detection of primate brain activation using a minimal-invasive procedure. The establishment of a minimal-invasive procedure for research is important because it reduces the stress on the animal while studies can be carried out in a friendly environment to produce highly reliable results. The underlying hypothesis of this proposal is that PAI can reliably detect primary motor cortex activation associated with forelimb movement in rhesus macaques through the dura matter, and provide depth-resolved functional information. The specific aims of this study include (1) to construct a PAI system for monkey brain cortex imaging; (2) to produce both structural and functional images on monkey brain cortex; (3) to compare the sensitivity and resolution of PAI with other established techniques. PUBLIC HEALTH RELEVANCE: In this project, we propose to test the feasibility of photoacoustic imaging technique for brain function imaging in non-human primates. With photoacoustic imaging, buried cortical structures might be imaged with optical contrast at ultrasound resolution. The success of this project will potentially contribute to studies involving stroke and Parkinson's disease in non- human primates.