Autophagy as a component of the innate immune response

自噬作为先天免疫反应的一个组成部分

• 批准号: 7837483
• 负责人: MICHELE Somes SWANSON
• 金额: $ 37.41万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-14 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7837483
• 关键词: Alveolar Macrophages; Amino Acids; Autophagocytosis; Autophagosome; Bacteria; Biological Assay; C57BL/6 Mouse; Caspase-1; Cell Death; Commit; Complex; Cytolysis; Cytoplasm; Data; Development; Dose; Enzyme-Linked Immunosorbent Assay; Epitopes; Flagellin; Fluorescence Microscopy; Genetic; Human; Immune response; Immune system; Infection; Inflammatory; Interleukin-18; Knowledge; Legionella pneumophila; Leukocytes; Life; Lung; Lysosomes; Mammalian Cell; Microbe; Microscopy; Molecular; Mus; Natural Immunity; Nutrient; Pathway interactions; Pattern; Proteins; Route; Signal Transduction; Starvation; Testing; Toll-like receptors; Virulence; Virulent; Withdrawal; abstracting; deprivation; macrophage; microbial; mutant; particle; pathogen; respiratory; response; tool; trait

Abstract An exciting development in the innate immunity field is the discovery that macrophages enlist autophagy to protect their cytoplasm from infection. Nutrient deprivation has long been known to induce autophagy, but how infection rapidly triggers this alternate route to digestive lysosomes is poorly understood. Genetic data indicate that mice resist L. pneumophila infection by relying on NOD-like proteins to detect cytosolic flagellin and then to digest the microbes by autophagy or to commit a pro-inflammatory cell death known as pyroptosis. To determine how macrophages integrate autophagy and pyroptosis as barriers to microbial infection, we will exploit Legionella pneumophila as a genetic probe of the innate immune system.

摘要 先天免疫领域的一个令人兴奋的发展是发现巨噬细胞 利用自噬来保护它们的细胞质不受感染。营养匮乏由来已久 已知可诱导自噬，但感染是如何迅速触发这一替代途径的 消化溶酶体知之甚少。遗传数据表明，小鼠对L. 依赖NOD样蛋白检测胞液鞭毛和 然后通过自噬来消化微生物，或者进行促炎细胞死亡 被称为上睑下垂。为了确定巨噬细胞如何整合自噬和 嗜肺军团菌作为微生物感染的屏障，我们将利用军团菌作为 先天免疫系统的遗传探针。

项目成果

Inflammasome components coordinate autophagy and pyroptosis as macrophage responses to infection.

• DOI: 10.1128/mbio.00620-12
• 发表时间: 2013-02-12
• 期刊: mBio
• 影响因子: 6.4
• 作者: Byrne BG;Dubuisson JF;Joshi AD;Persson JJ;Swanson MS
• 通讯作者: Swanson MS

Secrets of a successful pathogen: legionella resistance to progression along the autophagic pathway.

• DOI: 10.3389/fmicb.2011.00138
• 发表时间: 2011
• 期刊: Frontiers in microbiology
• 影响因子: 5.2
• 作者: Joshi AD;Swanson MS
• 通讯作者: Swanson MS