Core--Quantitative Molecular Assay

核心--定量分子检测

• 批准号: 7812199
• 负责人: Kenneth Hadlock
• 金额: $ 9.76万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7812199
• 关键词: Actins; Air; Aliquot; Alzheimer' s Disease; Amyotrophic Lateral Sclerosis; Anabolism; Animal Model; Biological Assay; Buffers; Cell Count; Cell Line; Cell Separation; Cells; Centrifugation; Classification; Clinical; Complementary DNA; Computer software; Cost Savings; DNA; DNA copy number; Data; Deoxyribonucleases; Detection; Diagnostic; Diploidy; Disease; Drug Delivery Systems; Edetic Acid; Endopeptidase K; Ensure; Ethanol; FCGR3B gene; Fostering; Genes; Genome; Genomics; Glycogen; Guanidinium Chloride; HIV; Heating; Human; Hydroxymethylbilane Synthase; Ice; Incubated; Individual; Inflammation; Instruction; Investigation; Label; Location; Macaca mulatta; Manufacturer Name; Manuscripts; Measurement; Methods; Molecular; Monitor; Neurodegenerative Disorders; Nucleic Acids; Pathogenesis; Patients; Performance; Phase I Clinical Trials; Plasma; Polyamines; Precipitation; Preparation; Program Research Project Grants; Protocols documentation; RNA; RNA-Directed DNA Polymerase; Reaction; Reagent; Recommendation; Reproducibility; Research Project Grants; Research Proposals; Reverse Transcriptase Polymerase Chain Reaction; Reverse Transcription; Rhodamine; Rhodamines; Ribonucleases; Role; Running; SIV; SYBR Green I; Sampling; Scheme; Services; Signal Transduction; Site; Sum; Supervision; Technology; Time; Tissues; Tube; Variant; Virion; Voting; Water; Weight; austin; guanidine thiocyanate; indexing; inhibitor/antagonist; interest; macrophage; melting; monocyte; nervous system disorder; present value; prognostic; programs; receptor; uracil-DNA glycosylase

In HIV associated neurological disease (HAND), HIV infected macrophages- and Fcgamma receptor III (CD16) positive macrophages and monocytes in particular, have been associated with disease pathogenesis. However, the differences between normal CD16+ monocytes that appear to have a role in replenishing tissue macrophages and dysfunctional CD16+ monocytes that appear to foster inflammation are not well defined. Pathologica LLC has recently developed a very sensitive molecular assay for the detection of the dysfunctional macrophage subset associated with severe neurodegenerative disease and by inference HIV associated neurological disease. In this program project grant all of the research proposals will make extensive use of this assay in the course of their investigations. Accordingly performance of this multi-gene real time quantitative reverse-transcription-PCR assay will be centralized at Pathologica during the course of this program project. Additionally, Pathologica will provide quantitative HIV and SIV PCR assays for all sites, including HIV proviral load, SIV proviral load, and SIV RNA. These assays will allow for an unambiguous determination if treatment with polyamine biosynthesis inhibitors significantly impacts HIV or SIV replication. The performance of these HIV / SIV assays at one location will help ensure the reproducibility and quality of the assay data for all research projects, as well as results in some cost savings.

在HIV相关神经疾病（HAND）中，HIV感染的巨噬细胞和Fc γ受体III 特别是CD 16阳性巨噬细胞和单核细胞，与疾病相关 发病机制然而，正常的CD 16+单核细胞之间的差异似乎有一个作用， 补充组织巨噬细胞和功能失调的CD 16+单核细胞，似乎促进炎症， 没有很好的定义。Pathologica LLC最近开发了一种非常灵敏的分子检测方法， 与严重神经退行性疾病相关的功能失调的巨噬细胞亚群， HIV相关的神经系统疾病在这个项目中，所有的研究提案都将获得资助。 在他们的研究过程中广泛使用这种测定法。因此，这种多基因的性能 真实的实时定量逆转录-PCR检测将在 这个节目项目。此外，Pathologica将为所有人提供定量HIV和SIV PCR检测。 位点，包括HIV前病毒载量、SIV前病毒载量和SIV RNA。这些试验将允许 明确确定用多胺生物合成抑制剂治疗是否显著影响HIV或 SIV复制。在一个地点进行这些HIV / SIV检测将有助于确保 所有研究项目的分析数据的重现性和质量，以及一些成本节约的结果。