Neural Basis of Taste Elicited Ingestion and Rejection

味觉引起摄入和排斥的神经基础

• 批准号: 7903000
• 负责人: JOSEPH B TRAVERS
• 金额: $ 3.02万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-14 至 2010-12-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7903000
• 关键词: Address; Amino Acids; Amygdaloid structure; Animals; Anorexia Nervosa; Appetitive Behavior; Behavioral; Biological; Biological Models; Brain Stem; Cell Nucleus; Chronic; Complex; Consummatory Behavior; Consumption; Deglutition Disorders; Desire for food; Disease; Feeding behaviors; Food; Functional disorder; Glycine; Hypertension; Immediate-Early Genes; Immunohistochemistry; In Situ Hybridization; Infusion procedures; Ingestion; Interneurons; Jaw; Label; Lateral; Ligands; Liquid substance; Medial; Motor; Motor Neurons; Muscle Contraction; N-Methyl-D-Aspartate Receptors; Neuraxis; Neurologic; Neuromodulator; Neurons; Neurotransmitters; Nitric Oxide; Nucleus solitarius; Obesity; Oral; Pattern; Phase; Pontine structure; Prosencephalon; Quinine; Reflex action; Regulation; Reticular Formation; Rodent; Role; Sensory; Series; Signal Transduction; Site; Sodium Chloride; Soluble Guanylate Cyclase; Solutions; Source; Stimulus; Sucrose; System; Taste Perception; Techniques; Testing; Tongue; awake; base; energy balance; feeding; gamma-Aminobutyric Acid; hindbrain; immunoreactivity; neuromechanism; parvocellular; receptor; relating to nervous system; research study; response; retrograde transport

DESCRIPTION (provided by applicant): The objectives of this proposal are to determine neural substrates in the rodent hindbrain through which gustatory stimuli guide ingestive behavior. The regulation of ingestive behavior is exceedingly complex, involving widespread regions of the central nervous system but the basic regulatory function of taste to determine palatable from unpalatable stimuli is complete in the caudal brainstem. These consummatory behaviors of ingestion and rejection are essential to the homeostatic control of energy balance. Anatomical studies suggest that the lateral tegmental field of the medullary reticular formation is a multifunctional substrate, receiving afferent sensory signals from the gustatory region of the nucleus of the solitary tract and producing the appropriate oromotor patterns of ingestion or rejection. When this region is pharmacologically inactivated, taste-elicited ingestion and rejection reponses are eliminated. The proposed experiments combine several techniques to address two broad questions: the generality and spatial extent of the brainstem consummatory substrate and its underlying mechanisms. Increasing appetitive responses to different stimuli by pharmacological infusions into selected forebrain sites will be combined with reversible block of selected brainstem reticular formation sites to establish whether forebrain signals converge on a single hindbrain region. A second series of microinfusion studies will determine the role of inhibitory amino acids and nitric oxide on oromotor coordination and a related series of studies will use double-labeling immunohistochemistry combined with retrograde transport and expression of the immediate early gene c-fos produced by gustatory stimulation to identify the source of these neurotransmitters and their respective receptors on interneurons in the circuit. Diseases such as obesity, hypertension, anorexia nervosa and dysphagia all involve ingestive dysfunction. Understanding the neurological basis of the fundamental biological decision ingest or reject food will contribute to the eventual solution of these chronic debilitating disorders.

描述（由申请人提供）：该提案的目标是确定啮齿动物后脑中的神经底物，味觉刺激引导摄入行为。摄取行为的调节非常复杂，涉及中枢神经系统的广泛区域，但是在尾脑干中，味觉的基本调节功能是确定可口刺激的基本调节功能。这些摄入和拒绝的绝妙行为对于能量平衡的稳态控制至关重要。解剖学研究表明，髓质网状形成的横向盖场是一种多功能底物，从孤立区的细胞核中接收传入的感觉信号，并产生适当的摄入或抑制或抑制的口感模式。当该区域在药理学上灭活时，消除了味觉吸收的摄入和排斥回购。提出的实验结合了几种技术来解决两个广泛的问题：脑干完整底物及其潜在机制的一般性和空间范围。通过药理学输注到选定的前脑部位对不同刺激的食欲反应增加将与选定的脑干网状形成位点的可逆块结合在一起，以确定前脑信号是否在单个后部脑区域汇聚。第二系列的微输注研究将确定抑制性氨基酸和一氧化物对口量配位的作用，一系列相关的研究将使用双标记的免疫组织化学，结合了逆行运输和直接的早期基因C-FOS的表达，从而通过阵发刺激来识别这些神经透射术的源及其互动，以识别这些神经透射剂的源。肥胖，高血压，神经性厌食症和吞咽困难等疾病均涉及摄入功能障碍。了解基本生物决策或拒绝食物的神经系统基础将有助于这些长期衰弱的疾病的最终解决方案。

项目成果

Medullary reticular formation activity during ingestion and rejection in the awake rat.

清醒大鼠摄入和排斥过程中的髓质网状形成活动。

• DOI: 10.1007/s002219900223
• 发表时间: 2000
• 期刊: Experimental brain research
• 影响因子: 2
• 作者: Travers,JB;DiNardo,LA;Karimnamazi,H
• 通讯作者: Karimnamazi,H

A computational model for motor pattern switching between taste-induced ingestion and rejection oromotor behaviors.

味觉诱导的摄入和拒绝口腔运动行为之间运动模式切换的计算模型。

• DOI: 10.1007/s10827-006-0009-3
• 发表时间: 2007
• 期刊: Journal of computational neuroscience
• 影响因子: 1.2
• 作者: Venugopal,Sharmila;Travers,JosephB;Terman,DavidH
• 通讯作者: Terman,DavidH

Intrinsic membrane properties of pre-oromotor neurons in the intermediate zone of the medullary reticular formation.

髓质网状结构中间区前口腔运动神经元的内在膜特性。

• DOI: 10.1016/j.neuroscience.2010.03.036
• 发表时间: 2010
• 期刊: Neuroscience
• 影响因子: 3.3
• 作者: Venugopal,S;Boulant,JA;Chen,Z;Travers,JB
• 通讯作者: Travers,JB

Distribution of fos-like immunoreactivity in the medullary reticular formation of the rat after gustatory elicited ingestion and rejection behaviors.

味觉诱发摄入和排斥行为后，大鼠髓质网状结构中类 fos 免疫反应性的分布。

• DOI: 10.1523/jneurosci.17-10-03826.1997
• 发表时间: 1997
• 期刊: The Journal of neuroscience : the official journal of the Society for Neuroscience
• 作者: DiNardo,LA;Travers,JB
• 通讯作者: Travers,JB