STRUCTURAL STUDIES OF GLYCOLIPID-REACTIVE T CELLS AND ANTIBODIES

糖脂反应性 T 细胞和抗体的结构研究

• 批准号: 8170083
• 负责人: Dirk M Zajonc
• 金额: $ 0.34万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170083
• 关键词: Adjuvant; Agonist; Animal Model; Antibodies; Antigen Presentation; Antigens; B-Lymphocytes; Borrelia burgdorferi; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Development; Epitopes; Family; Funding; Glycolipids; Grant; Hand; Immune; Immune system; Institutes; Institution; Lipid Binding; Lipids; Lyme Disease; Multiple Sclerosis; Mus; Patients; Research; Research Personnel; Resources; Source; T-Lymphocyte; United States National Institutes of Health; Work; base; chemotherapeutic agent; immune function

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The CD1 family of antigen presenting molecules presents a wide variety of glycolipid antigens to lipid-specific T cells, resulting in their activation and expansion, necessary to carry out various immune functions including the activation of other immune cells. Structural studies of various CD1-lipid-TCR complexes will greatly enhance our understanding of glycolipid antigen presentation and recognition in the immune system. The gained structural results can potentially be used for the development of lipid based chemotherapeutic agents or adjuvants to target various immune conditions, such as Multiple Sclerosis (MS) or lyme disease. The immunomodulatory functions of these compounds can be assessed using animal models in our institute. In that regard we are working on one hand with T cells that are enriched in MS patients and on the other hand we have already crystallized mouse CD1d with a potential T cell agonist from Borrelia burgdorferi, the causative agent of Lyme disease. Furthermore we have obtained antibodies, which can discriminate between different CD1d-bound lipids. A ternary complex between CD1d-lipid-Fab will provide a detailed comparison of the recognition and interaction with specific glycolipid epitopes between T cells and B cells.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 抗原分子的CD1家族为脂质特异性T细胞提供了各种各样的糖脂抗原，从而导致其激活和膨胀，这是执行各种免疫功能所必需的，包括激活其他免疫细胞。各种CD1-脂质-TCR复合物的结构研究将大大增强我们对免疫系统中糖脂抗原表现和识别的理解。获得的结构结果可能有可能用于开发脂质化学治疗剂或辅助药物，以靶向各种免疫疾病，例如多发性硬化症（MS）或莱姆病。这些化合物的免疫调节功能可以使用我们研究所的动物模型来评估。在这方面，我们正在与富含MS患者的T细胞一起工作，另一方面，我们已经用来自Borlelia burgdorferi的潜在T细胞激动剂结晶了小鼠CD1D，Borrelia burgdorferi是莱姆病的病因。此外，我们获得了可以区分不同CD1D结合脂质的抗体。 CD1D-脂肪射击之间的三元复合物将对与T细胞和B细胞之间的特定糖脂表位的识别和相互作用进行详细的比较。