Single nucleotide polymorphisms of neuronal CACNA1C L-type calcium channels assoc

神经元CACNA1C L型钙通道关联的单核苷酸多态性

• 批准号: 7995239
• 负责人: Diane Lipscombe
• 金额: $ 20.05万
• 依托单位: BROWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-01 至 2012-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7995239
• 关键词: Adult; Adverse effects; Affect; Alternative Splicing; Ankyrins; Architecture; Attention; Behavior; Behavioral; Binding; Binding Sites; Bipolar Disorder; Brain; Brain imaging; Brain region; Calcium; Calcium Channel; Calcium Channel Blockers; Cardiac; Cell Death; Cell Nucleus; Cell physiology; Cells; Chromosomes, Human, Pair 12; Chronic; Comorbidity; Data; Development; Disease; Drug Delivery Systems; Embryo; Event; Exons; Funding Mechanisms; Gene Combinations; Gene Expression; Genes; Heart Diseases; Hereditary Disease; Hippocampus (Brain); Human; Human Chromosomes; Hypertension; Individual; Introns; Knockout Mice; L-Type Calcium Channels; Link; Lithium; Manic; Maps; Mental Depression; Mental disorders; Messenger RNA; Molecular; Mus; Muscle Contraction; Mutation; Neurons; Nucleotides; Patients; Pattern; Population Study; Positioning Attribute; Predisposition; RNA; RNA Splicing; RNA-Binding Proteins; Reading; Regulatory Element; Risk; Risk Factors; Role; Safety; Second Messenger Systems; Signal Transduction; Single Nucleotide Polymorphism; Single Nucleotide Polymorphism Map; Site; Staging; Structure; Surface; Synapses; System; Testing; Time; Timothy syndrome; Translations; United States; Variant; Work; base; common treatment; density; disorder control; genome wide association study; high risk; insight; interest; mRNA Precursor; nervous system disorder; neuronal growth; neurotransmitter release; novel; painful neuropathy; postsynaptic; protein expression; public health relevance; second messenger; theories; voltage

DESCRIPTION (provided by applicant): This is a new R21 application to study the role of a neuronal calcium channel in determining susceptibility to bipolar disorder. All excitable cells use calcium ion channels on their surface to read electrical signals and convert them into a change in intracellular calcium, a ubiquitous second messenger. Calcium controls a huge number of cellular processes including muscle contraction, neurotransmitter release, cell death and neuronal growth. Calcium ion channels are important drug targets for treating hypertension and neuropathic pain. Mutations in the CACNA1C calcium ion channel gene cause a rare hereditary disorder Timothy Syndrome and single nucleotide polymorphisms identified very recently in the same CACNA1C gene associate with bipolar disorder. Bipolar disorder is a chronic mental illness affecting close to 6 million adults in the United States characterized by cyclical episodes of mania and depression. The most common treatment is lithium but this agent is only partially effective, has numerous side effects, and a low safety margin. There is a clear inheritable risk in bipolar disorder. Recently a large, collaborative genome-wide association study analyzed >10,000 bipolar and control individuals and identified a region in human chromosome 12 that has significant association with bipolar disorder. Bipolar disease-associated single nucleotide polymorphisms mapped to a long intron in an uncharacterized region of the CACNA1C gene. This exciting discovery affords us a unique opportunity to understand how single nucleotide variations in the CACNA1C gene could disrupt normal calcium channel activity in the brain. We will use a combination of gene and RNA analyses, and electrophysiological recordings to explore how this potential site of bipolar susceptibility in CACNA1C controls calcium ion channel function. Our work has the potential to provide novel insights into the molecular mechanisms underlying bipolar disorder. ) PUBLIC HEALTH RELEVANCE: This R21 project will test the hypothesis that a long intron in the CACNA1C gene, recently identified as a risk factor in bipolar disorder, controls calcium channel function through alternative pre-mRNA splicing. We will use a combination of RNA, gene, and electrophysiological analyses to reveal the functional role of intron 4 in controlling L-type calcium channel activity in neurons.

描述(由申请人提供)：这是一项新的R21申请，研究神经元钙通道在确定双相情感障碍易感性中的作用。所有可兴奋的细胞都使用其表面的钙离子通道来读取电信号，并将其转化为细胞内钙的变化，钙是普遍存在的第二信使。钙控制着大量的细胞过程，包括肌肉收缩、神经递质释放、细胞死亡和神经元生长。钙离子通道是治疗高血压和神经病理性疼痛的重要药物靶点。CACNA1C钙离子通道基因突变导致一种罕见的遗传性疾病Timothy综合征，最近发现同一CACNA1C基因中的单核苷酸多态与双相情感障碍有关。双相情感障碍是一种慢性精神疾病，影响着美国近600万成年人，其特征是躁狂和抑郁的周期性发作。最常见的治疗方法是锂，但这种药物只有部分有效，副作用众多，安全边际低。双相情感障碍存在明显的遗传风险。最近，一项大型的全基因组协作性研究分析了10,000名双相情感障碍和对照个体，并确定了人类12号染色体上与双相情感障碍有显著关联的区域。与双相情感疾病相关的单核苷酸多态定位于CACNA1C基因一个未特定区的长内含子。这一令人兴奋的发现为我们提供了一个独特的机会来了解CACNA1C基因的单核苷酸变异如何扰乱大脑中正常的钙通道活动。我们将结合基因和RNA分析以及电生理记录来探索CACNA1C中这个潜在的双极易感部位是如何控制钙离子通道功能的。我们的工作有可能为双相情感障碍的分子机制提供新的见解。) 公共卫生相关性：这个R21项目将测试CACNA1C基因中的一个长内含子的假设，该基因的一个长内含子最近被确定为双相情感障碍的危险因素，通过替代的前mRNA剪接控制钙通道功能。我们将结合核糖核酸、基因和电生理分析来揭示内含子4在控制神经元L型钙通道活动中的功能作用。