Electromechanical Imaging of Live Vascular Smooth Muscle Cells

活血管平滑肌细胞的机电成像

• 批准号: 8019005
• 负责人: Alexey A Vertegel
• 金额: $ 18.12万
• 依托单位: CLEMSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8019005
• 关键词: Address; Atherosclerosis; Atomic Force Microscopy; Behavior; Biological; Biological Models; Biological Process; Biosensing Techniques; Blood Vessels; Calcified; Cell Culture Techniques; Cells; Collagen Fibril; Computer software; Coupling; Culture Media; Development; Diagnosis; Diagnostic; Diagnostic Procedure; Electrolytes; Electron Beam; Elements; Environment; Functional disorder; Future; Goals; Heart; Image; Individual; Injury; Investigation; Ion Channel; Length; Life; Liquid substance; Maps; Mechanics; Medical; Methodology; Methods; Microfilaments; Microtubules; Modeling; Molecular; Motor; Muscle Cells; Patch-Clamp Techniques; Pathologic Processes; Phenotype; Physiological; Play; Process; Property; Proteins; Protons; Reaction; Resolution; Role; Scanning Probe Microscopy; Smooth Muscle Myocytes; Solutions; Stimulus; Structure; System; Techniques; Testing; Tissue Engineering; Tissues; Vision; aqueous; base; biological systems; cell behavior; cell type; design; electric field; instrument; laminin-5; member; molecular shape; nanoscale; novel diagnostics; novel therapeutic intervention; operation; prevent; public health relevance; response; restenosis; submicron; success; tool; voltage

DESCRIPTION (provided by applicant): Coupling between electrical and mechanical phenomena is a universal feature of all biological systems. Yet, not much is known about origins of biological electromechanical phenomena on the cellular and subcellular scale. Understanding the underlying molecular mechanisms may have tremendous impact on general understanding of biological processes and specific biomedical applications. Electromechanical stimulation of cells can become a valuable tool for their characterization and can eventually result in the development of novel therapeutic interventions. Insufficient information about electromechanical phenomena in biological systems is a result of the lack of characterization techniques capable of providing such information on the nanometer scale and capable of operation in liquid environment. Recently, piezoresponse force microscopy (PFM) has demonstrated potential for imaging structure of connective and calcified tissues with sub-10 nm resolution. Members of this team have also demonstrated high resolution piezoresponse imaging of model systems in aqueous solutions. The ability to map electromechanical properties in aqueous media opens the way to characterization of biological systems in native-like conditions. Here we propose to expand PFM for characterization and stimulation of live cells in physiological environment. Our long-term vision is to use electromechanical imaging as a diagnostic tool and ultimately, utilize electromechanical stimulation for induction of a desirable change in cell behavior. More specifically, we will focus on electromechanical properties of vascular smooth muscle cells as a model system. To accomplish the goals of this project, we will use electron-beam induced etching to fabricate shielded probes needed for PFM imaging in electrolyte solutions with physiological concentrations. We will perform PFM imaging of live VSMCs in aqueous media and compare piezoresponce of their synthetic and contractile phenotypes. As a result of this project we expect to develop experimental technique needed for investigation of local electromechanical phenomena in live cells and create a methodology for predicting cell behavior upon electromechanical stimulation. Public Health Relevance Statement: Electromechanical imaging of live cells can become a unique method to provide information about electrophysiological response of cells and tissues on the nanoscale. It can also be used in the future for diagnostic purposes. Even more broadly, it is envisioned that the use of electromechanical stimulations of live cells can find applications in tissue engineering, medical diagnosis, and biosensing.

描述（由申请人提供）：电气和机械现象之间的耦合是所有生物系统的普遍特征。然而，对细胞和亚细胞尺度上的生物机电现象的起源知之甚少。了解潜在的分子机制可能对生物过程和特定生物医学应用的一般理解产生巨大影响。细胞的机电刺激可以成为表征它们的有价值的工具，并最终导致新的治疗干预措施的发展。

项目成果

Electromechanical and elastic probing of bacteria in a cell culture medium.

• DOI: 10.1088/0957-4484/23/24/245705
• 发表时间: 2012-06-22
• 期刊: Nanotechnology
• 影响因子: 3.5
• 作者: Thompson GL;Reukov VV;Nikiforov MP;Jesse S;Kalinin SV;Vertegel AA
• 通讯作者: Vertegel AA

Double-layer mediated electromechanical response of amyloid fibrils in liquid environment.

• DOI: 10.1021/nn901127k
• 发表时间: 2010-02-23
• 期刊: ACS nano
• 影响因子: 17.1
• 作者: Nikiforov MP;Thompson GL;Reukov VV;Jesse S;Guo S;Rodriguez BJ;Seal K;Vertegel AA;Kalinin SV
• 通讯作者: Kalinin SV

Synthesis and electroplating of high resolution insulated carbon nanotube scanning probes for imaging in liquid solutions.

• DOI: 10.1088/0957-4484/23/14/145301
• 发表时间: 2012-04-13
• 期刊: Nanotechnology
• 影响因子: 3.5
• 作者: Roberts NA;Noh JH;Lassiter MG;Guo S;Kalinin SV;Rack PD
• 通讯作者: Rack PD

Nanofabrication of insulated scanning probes for electromechanical imaging in liquid solutions.

• DOI: 10.1088/0957-4484/21/36/365302
• 发表时间: 2010-09-10
• 期刊: Nanotechnology
• 影响因子: 3.5
• 作者: Noh JH;Nikiforov M;Kalinin SV;Vertegel AA;Rack PD
• 通讯作者: Rack PD

Functional recognition imaging using artificial neural networks: applications to rapid cellular identification via broadband electromechanical response.

• DOI: 10.1088/0957-4484/20/40/405708
• 发表时间: 2009-10-07
• 期刊: Nanotechnology
• 影响因子: 3.5
• 作者: Nikiforov MP;Reukov VV;Thompson GL;Vertegel AA;Guo S;Kalinin SV;Jesse S
• 通讯作者: Jesse S