Reconstructing Sub-cellular Structure using Plasmonic Metamaterials

摘要： 用等离子体超材料重建亚细胞结构 解析细胞内结构的能力为破译 复杂的化学相互作用，将细胞器的组成和组织与 细胞过程，以及观察病毒对细胞的感染。该项目描述了 等离子体超材料如何在三维（3D）中解析亚细胞结构， 亚衍射分辨率，并且不使用荧光标记。这些新型金属 纳米结构可以与标准光学显微镜集成。因为那道光芒 从金属膜中出现的有限数量的纳米孔（补丁）是周期性的 在3D中调制，这些等离子体超材料可以用于确定 像细胞一样的厚而透明的样品。与传统光学不同， 远离物体，这些平面金属透镜结构可以直接构造在玻璃上 盖玻片因此，可以实现更高的分辨率，因为光不会事先分散。 撞击样品之前或之后。我们建议研究两类等离子体激元 用于细胞的无标记成像的结构：（1）用微尺度贴片穿孔的金膜 纳米级孔，可以通过解构干涉图案来实现3D成像 从结构光束和（2）金金字塔颗粒，它可以用来识别 细胞内特定生物标志物的空间位置。

ABSTRACT: Reconstructing Sub-Cellular Structure with Plasmonic Metamaterials The ability to resolve intracellular structure has opened new opportunities for deciphering complex chemical interactions, for relating the composition and organization of organelles to cellular processes, and for observing the infection of cells by viruses. This project describes how plasmonic metamaterials can resolve sub-cellular structure in three dimensions (3D), with sub-diffraction resolution, and without the use of fluorescent labels. These new types of metal nanostructures can be integrated with standard optical microscopes. Because the light that emerges from a metal film perforated with a finite number of nanoholes (a patch) is periodically modulated in 3D, these plasmonic metamaterials can be used for determining the structure of thick, transparent samples like cells. Unlike in conventional optics, where lenses are situated far from the object, these planar metallic lens structures can be constructed directly on glass coverslips. Hence, higher resolution can be achieved because the light does not disperse prior to or after impinging on the sample. We propose to investigate two classes of plasmonic structures for the label-free imaging of cells: (1) gold films perforated with microscale patches of nanoscale holes, which can achieve 3D imaging by deconstructing interference patterns from structured beams of light and (2) gold pyramidal particles, which can be used to identify the spatial locations of specific biomarkers within cells.