Cell based biosensor fabricated by soft Lithography

通过软光刻技术制造的基于细胞的生物传感器

• 批准号: 6515126
• 负责人: Teri Wang Odom
• 金额: $ 1.61万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-05 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6515126
• 关键词: angiogenesis; angiogenesis factor; angiogenesis inhibitors; apoptosis; bioassay; cell adhesion; cell cell interaction; extracellular matrix proteins; neoplastic cell; tissue /cell culture; vascular endothelium

Soft lithographic techniques are powerful tools to manipulate and pattern biomaterials on the micrometer scale. These methods allow chemical and biological studies on well-defined, confined regions of surfaces. Microcontact printing and patterning by microfluidic channels will be combined to fabricate cell-based biosensors that will be further utilized to investigate cell behavior. This bioassay will ultimately assist in detection and analysis of diseases. The capabilities and effectiveness of the cell bioassay will be tested through proof of concept demonstrations of cell-protein adhesion and adsorption. Also, this cell-based biosensor will enable investigations of angiogenesis and blood vessel formation in bovine capillary endothelial (BCE) cells. Changes in cell morphology due to the presence of angiogenic growth factors and inhibitors will be studied in parallel channels on a single chip. Moreover, differentiation, capillary tube formation, in BCE cells will be investigated on patterned adhesive substrates. Finally, interactions between BCE cells and tumor cells will be probed to learn how their spreading is affected by the presence of either angiogenic promoters or suppressors. These initial experiments should provide insight on the proposed bioassay's potential and limitations, and how such a device may be exploited to investigate the effects of unknown biologically active molecules on cells.

软光刻技术是在微米尺度上操纵和图案化生物材料的强大工具。这些方法允许对明确的、有限的表面区域进行化学和生物学研究。微接触印刷和微流体通道图案化将结合起来制造基于细胞的生物传感器，该传感器将进一步用于研究细胞行为。这种生物测定最终将有助于疾病的检测和分析。细胞生物测定的能力和有效性将通过细胞-蛋白质粘附和吸附的概念验证演示进行测试。此外，这种基于细胞的生物传感器将能够研究牛毛细血管内皮（BCE）细胞的血管生成和血管形成。由于血管生成生长因子和抑制剂的存在而导致的细胞形态的变化将在单个芯片上的并行通道中进行研究。此外，BCE 细胞的分化、毛细管形成将在图案化的粘合基底上进行研究。最后，将探讨 BCE 细胞和​​肿瘤细胞之间的相互作用，以了解血管生成启动子或抑制子的存在如何影响它们的扩散。这些初步实验应该可以深入了解所提出的生物测定的潜力和局限性，以及如何利用这种设备来研究未知生物活性分子对细胞的影响。