Self Healing Biomaterials

描述（由申请人提供）：机械故障的原位修复在生物材料研究中仍然是一个很大程度上未经检验的领域。材料科学的一个新兴领域是“自我修复材料”（SHM），它使用各种嵌入的化学物质在微裂纹合并成扩展裂纹之前原位检测和修复微裂纹。本R21提案探讨了将生物相容性、无毒SHM技术与最简单的承重生物材料配方之一——双组分丙烯酸骨水泥——结合的可行性，并采用了最直接的SHM技术——分散在催化剂嵌入聚合物基质中的微囊化愈合剂。这项研究包括四个具体目标。目的1：利用乳化油水界面聚合制备微囊化氰基丙烯酸烷基酯愈合剂。目的2：将微囊化愈合剂掺入双组分PMMA基质中。目的3：表征自愈合骨水泥力学性能和断裂力学。目的4：通过小鼠成纤维细胞培养中提取培养基的洗脱测试和人成骨细胞培养中骨水泥样品上的细胞生长测试，对自愈骨水泥进行细胞毒性测试。

DESCRIPTION (provided by applicant): In situ repair of mechanical failure remains a largely unexamined area of study in biomaterials. A newly emerging area of materials science is "Self-Healing Materials" (SHM) that uses a variety of embedded chemistries to detect and repair microcracks in situ before they coalesce into propagating cracks. This R21 proposal examines the feasibility of incorporating biocompatible, non-toxic SHM technology into one of the simplest load-bearing biomaterial formulations -- two component acrylic bone cement -- with the most straightforward SHM technology -- microencapsulated healing agent dispersed in a catalyst-embedded polymer matrix. The study consists of four specific aims. Aim 1: Fabrication of microencapsulated alkyl cyanoacrylate healing agent using emulsified oil in water interfacial polymerization. Aim 2: Incorporation of the icroencapsulated healing agent into the two-component PMMA matrix. Aim 3: Characterization of self-healing bone cement mechanical properties and fracture mechanics. Aim 4: Cytotoxicity testing of self-healing bone cement by elution testing of extraction media in mouse fibroblast culture, and by cell ongrowth onto samples of bone cement placed in cultures of human osteoblasts. PUBLIC HEALTH RELEVANCE: The most likely mode of failure of all cyclically loaded biomaterials is fatigue failure resulting from the accumulation of microcracks. The long-term goal of this study is to develop composite biomaterials that have the capacity to repair failure at the microcrack level and thus prolong the use life of cyclically loaded biomaterials. This project specifically tests out the concept feasibility using acrylic bone cement as a model biomaterial.