Genetic Environmental Relationship Between Bone Obesity and Leptin in Mice
Genetic Environmental Relationship Between Bone Obesity and Leptin in Mice
批准号:
8013377
负责人:
JAMES M CHEVERUD
金额:
$3.0万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2010
资助国家:
美国
项目状态:
已结题
起止时间:
2010-02-10 至 2011-01-31
关键词:
AffectAgeAnimal ModelAnimalsAutopsyBioinformaticsBiologicalBiological AssayBiomechanicsBrainCandidate Disease GeneCharacteristicsDiabetes MellitusDietDietary FactorsDietary FatsDiseaseElderlyEnvironmentEnvironmental Risk FactorEvaluationFamily history ofFastingFat-Restricted DietFatty acid glycerol estersFractureGenderGenerationsGenesGeneticGenetic PolymorphismGenetic Predisposition to DiseaseGenetic VariationGenomeGenomicsGenotypeGoalsHealthHeritabilityIndividualInheritedKidneyKnock-outLeadLeptinLightLiverLocationMapsMeasuresMethodsModelingMorphologic artifactsMorphologyMouse StrainsMusMutant Strains MiceNormal RangeObesityOsteoporosisPathway interactionsPhenotypePhysiologicalPopulationPropertyQuantitative Trait LociRecombinantsRegulationResearch PersonnelRiskRisk FactorsRoleSM/J MouseSeriesSignal TransductionSkeletonSpecific qualifier valueTestingTissuesTransgenic MiceVariantWeightWomanWorkbasebonebone massbone strengthcohortcostexperiencefeedinggene environment interactionhigh standardinterestmenmouse modelnovelosteoporosis with pathological fractureoverexpressionprogramsresearch studyresponsesextrait
中文摘要
骨质疏松症和随之而来的骨折是老年人面临的一个重要健康问题。四大风险
骨质疏松症的因素是年龄、性别、遗传倾向和肥胖。在这里,我们建议检查
影响骨量、形态和生物力学特性的饮食和遗传因素,
在已建立的肥胖、糖尿病和饮食性肥胖小鼠模型中,
反应,LG/J和SM/J小鼠品系的交叉。通过这些研究我们将发现新的基因
影响骨骼的途径及其与肥胖的关系。我们将测量骨骼的遗传性水平
LGXSM重组近交系(R1)中的性状及其与肥胖和瘦素水平的遗传相关性
菌株来自每个品系的动物被喂食高脂肪或低脂肪饮食,使我们能够检查
基因和环境(膳食脂肪)对骨特征和骨肥胖关系的影响。我们将
确定影响骨骼的基因组区域(数量性状基因座,QTL)及其与肥胖的关系,
Rl品系、F2互交和F10高级互交(Al)品系群体。饲养所有动物,
基因分型为以前的项目,并提供给这个项目在没有额外的费用。我们还将精细映射
将这些QTL在A1系的F32代(N = 1000)中进行验证和精细定位
早期的人口。与F2和F10代不同,完全肥胖、瘦素和糖尿病相关的
将在F32动物中收集表型,并且动物将以高脂肪或低脂肪饮食饲养。
在这一代的精细定位将使我们能够确定一个小的位置候选基因的集合,
进一步评价。将检查这些基因的序列和表达多态性。强烈
将在敲除和过表达转基因小鼠中评价支持的候选基因。这个项目
将确定影响骨质疏松症的新基因和生理途径,并研究遗传和
基于环境的肥胖影响骨量、形态和生物力学功能。
英文摘要
Osteoporosis and subsequent fractures are an important health problem facing the elderly. Four major risk
factors for osteoporosis are age, gender, genetic predisposition, and obesity. Here we propose to examine
dietary and genetic factors affecting bone mass, morphology, and biomechanical properties and the
relationships of these features to obesity in an established mouse model for obesity, diabetes, and dietary
response, the cross of LG/J and SM/J mouse strains. Through these studies we will discover novel genes
and pathways affecting bone and its relationship to obesity. We will measure the level of heritability for bone
traits and their genetic correlations with obesity and leptin levels in the LGXSM Recombinant Inbred (Rl)
strains. Animals from each strain have been fed a high or low fat diet allowing us to examine the effects of
both genes and environment (dietary fat) on bone characteristics and bone-obesity relationships. We will
identify genomic regions (Quantitative Trait Loci, QTLs) affecting bone and its relationship to obesity in the
Rl strains, F2 intercross, and an F10 Advanced Intercross (Al) line population. All animals were reared and
genotyped for previous projects and are available to this project at no additional cost. We will also fine-map
these QTLs in the F32 generation of the Al Line (N = 1000) to validate and fine-map the QTLs discovered
with earlier populations. Unlike the F2 and F10 generations, complete obesity, leptin, and diabetes-related
phenotypes will be collected in the F32 animals and animals will be reared on either a high or low fat diet.
Fine-mapping in this generation will allow us to identify a small set of positional candidate genes for for
further evaluation. Sequence and expression polymorphism will be examined for these genes. Strongly
supported candidate genes will be evaluated in knock-out and overexpressing transgenic mice. This project
will identify novel genes and physiological pathways affecting osteoporosis and examine how genetic and
environmentally-based obesity affects bone mass, morphology and biomechanical function.
期刊论文(3)
专著(0)
科研奖励(0)
会议论文
DOI:
10.1172/jci175282
发表时间:
2023-12-01
期刊:
JOURNAL OF CLINICAL INVESTIGATION
影响因子:
15.9
作者:
[Cypess, Aaron M.]
通讯作者:
Cypess, Aaron M.
Genetics of Cartilage Regeneration and Osteoarthritis
-
批准号:8927814
-
项目类别:
-
资助金额:$10.0万
-
财政年份:2013
-
负责人:JAMES M CHEVERUD
-
依托单位:
Genetics of Cartilage Regeneration and Osteoarthritis
-
批准号:8579593
-
项目类别:
-
资助金额:$30.4万
-
财政年份:2013
-
负责人:JAMES M CHEVERUD
-
依托单位:
Genetics of Cartilage Regeneration and Osteoarthritis
-
批准号:8740154
-
项目类别:
-
资助金额:$30.4万
-
财政年份:2013
-
负责人:JAMES M CHEVERUD
-
依托单位:
A Mouse Model For Complex Human Diseases
-
批准号:7909226
-
项目类别:
-
资助金额:$15.39万
-
财政年份:2009
-
负责人:JAMES M CHEVERUD
-
依托单位:
Genetic Mouse Models of Cartilage Healing
-
批准号:7853233
-
项目类别:
-
资助金额:$66.89万
-
财政年份:2009
-
负责人:JAMES M CHEVERUD
-
依托单位:
Genetic Mouse Models of Cartilage Healing
-
批准号:7941043
-
项目类别:
-
资助金额:$103.68万
-
财政年份:2009
-
负责人:JAMES M CHEVERUD
-
依托单位:
GENETICS OF SCHIZOPHRENIA-RELATED TRAITS IN MICE
-
批准号:7476364
-
项目类别:
-
资助金额:$41.11万
-
财政年份:2007
-
负责人:JAMES M CHEVERUD
-
依托单位:
Genetic Environmental Relationship Between Bone, Obesity and Leptin in Mice
-
批准号:7220536
-
项目类别:
-
资助金额:$29.93万
-
财政年份:2006
-
负责人:JAMES M CHEVERUD
-
依托单位:
Genetic Environmental Relationship Between Bone Obesity and Leptin in Mice
-
批准号:7585768
-
项目类别:
-
资助金额:$29.31万
-
财政年份:2006
-
负责人:JAMES M CHEVERUD
-
依托单位:
Genetic Environmental Relationship Between Bone Obesity and Leptin in Mice
-
批准号:7385994
-
项目类别:
-
资助金额:$29.31万
-
财政年份:2006
-
负责人:JAMES M CHEVERUD
-
依托单位:
Gene Environment Relationship Btwn Bone, Obesity, Leptin
-
批准号:7083420
-
项目类别:
-
资助金额:$30.92万
-
财政年份:2006
-
负责人:JAMES M CHEVERUD
-
依托单位:
GENETIC VARIATION IN MURINE LONG BONE GROWTH AND DEVELOPMENT
-
批准号:7147809
-
项目类别:
-
资助金额:$33.55万
-
财政年份:2006
-
负责人:JAMES M CHEVERUD
-
依托单位:
GENETIC VARIATION IN MURINE LONG BONE GROWTH AND DEVELOPMENT
-
批准号:7257176
-
项目类别:
-
资助金额:$32.47万
-
财政年份:2006
-
负责人:JAMES M CHEVERUD
-
依托单位:
GENETIC VARIATION IN MURINE LONG BONE GROWTH AND DEVELOPMENT
-
批准号:7446673
-
项目类别:
-
资助金额:$31.82万
-
财政年份:2006
-
负责人:JAMES M CHEVERUD
-
依托单位:
Genetic Variation in Murine Long Bone Growth and Development
-
批准号:7869307
-
项目类别:
-
资助金额:$31.5万
-
财政年份:2006
-
负责人:JAMES M CHEVERUD
-
依托单位:
Genetic Variation in Murine Long Bone Growth and Development
-
批准号:7626358
-
项目类别:
-
资助金额:$31.82万
-
财政年份:2006
-
负责人:JAMES M CHEVERUD
-
依托单位:
GENETICS OF SCHIZOPHRENIA-RELATED TRAITS IN MICE
-
批准号:6969035
-
项目类别:
-
资助金额:$22.95万
-
财政年份:2004
-
负责人:JAMES M CHEVERUD
-
依托单位:
A Mouse Model For Complex Human Diseases
-
批准号:7467915
-
项目类别:
-
资助金额:$36.28万
-
财政年份:2001
-
负责人:JAMES M CHEVERUD
-
依托单位:
A Mouse Model For Complex Human Diseases
-
批准号:7237952
-
项目类别:
-
资助金额:$37.02万
-
财政年份:2001
-
负责人:JAMES M CHEVERUD
-
依托单位:
A Mouse Model for Complex Human Diseases
-
批准号:6639880
-
项目类别:
-
资助金额:$31.92万
-
财政年份:2001
-
负责人:JAMES M CHEVERUD
-
依托单位:
国内基金
海外基金
登录
查看更多内容
补阳还五汤通过AGE-RAGE通路调控脓毒症免疫失衡的机制与转化研究
-
批准号:JCZRLH202601523
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2026
-
负责人:
-
依托单位:
靶向递送一氧化碳调控AGE-RAGE级联反应促进糖尿病创面愈合研究
-
批准号:JCZRQN202500010
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2025
-
负责人:
-
依托单位:
对香豆酸抑制AGE-RAGE-Ang-1通路改善海马血管生成障碍发挥抗阿尔兹海默病作用
-
批准号:2025JJ70209
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2025
-
负责人:雷芬芳
-
依托单位:
AGE-RAGE通路调控慢性胰腺炎纤维化进程的作用及分子机制
-
批准号:--
-
项目类别:面上项目
-
资助金额:--
-
批准年份:2024
-
负责人:万荣
-
依托单位:
甜茶抑制AGE-RAGE通路增强突触可塑性改善小鼠抑郁样行为
-
批准号:2023JJ50274
-
项目类别:省市级项目
-
资助金额:--
-
批准年份:2023
-
负责人:贺志明
-
依托单位:
蒙药额尔敦-乌日勒基础方调控AGE-RAGE信号通路改善术后认知功能障碍研究
-
批准号:--
-
项目类别:地区科学基金项目
-
资助金额:33万元
-
批准年份:2022
-
负责人:都义日
-
依托单位:
补肾健脾祛瘀方调控AGE/RAGE信号通路在再生障碍性贫血骨髓间充质干细胞功能受损的作用与机制研究
-
批准号:--
-
项目类别:面上项目
-
资助金额:52万元
-
批准年份:2022
-
负责人:叶宝东
-
依托单位:
LncRNA GAS5在2型糖尿病动脉粥样硬化中对AGE-RAGE 信号通路上相关基因的调控作用及机制研究
-
批准号:
-
项目类别:省市级项目
-
资助金额:10.0万元
-
批准年份:2022
-
负责人:于海兵
-
依托单位:
围绕GLP1-Arginine-AGE/RAGE轴构建探针组学方法探索大柴胡汤异病同治的效应机制
-
批准号:81973577
-
项目类别:面上项目
-
资助金额:55.0万元
-
批准年份:2019
-
负责人:辛贵忠
-
依托单位:
AGE/RAGE通路microRNA编码基因多态性与2型糖尿病并发冠心病的关联研究
-
批准号:81602908
-
项目类别:青年科学基金项目
-
资助金额:18.0万元
-
批准年份:2016
-
负责人:刘括
-
依托单位: