Computational and Biochemical Docking of Dopamine Transporter Antagonists
多巴胺转运蛋白拮抗剂的计算和生化对接
基本信息
- 批准号:8012807
- 负责人:
- 金额:$ 29.89万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-01-15 至 2014-12-31
- 项目状态:已结题
- 来源:
- 关键词:Active SitesBehavioralBindingBinding SitesBiochemicalBiological AssayChemicalsClinicalCluster AnalysisCocaineComputer SimulationComputing MethodologiesCysteineDataDigestionDockingDrug CompoundingFamily memberGenerationsHomology ModelingIntegral Membrane ProteinInvestigationLeadLibrariesLigand BindingLigandsLinkMental HealthMethionineMethodsModelingMolecularMolecular ModelsMutagenesisNeurologicOutcomePeptide MappingPharmaceutical PreparationsPlayPropertyProteinsRoleScanningSequence AlignmentSiteSite-Directed MutagenesisStructural ModelsStructureTestingTropanesaddictionanalogbaseclinically relevantcomparativecomputer studiescrosslinkdopamine transporterdrug of abusedrug seeking behaviorflexibilityinhibitor/antagonistmolecular dynamicsmolecular modelingmolecular transporternoradrenaline transporternovelprogramsprotein foldingpublic health relevanceserotonin transporteruptake
项目摘要
DESCRIPTION (provided by applicant): This project will investigate how drug compounds interact with dopamine transporter (DAT) utilizing an integrated approach combining biochemical and computational methodologies. Biochemical analyses will use novel photoaffinity inhibitors that irreversibly attach to the transporter. These studies will determine points of contact between the drug and DAT and the molecular orientation of the ligand in the binding site. In parallel, we will carry out computational studies to build comparative models of DAT based on homology to a competitor-bound crystal structure from a related bacterial transporter, LeuT. The comparative models will be used in conjunction with the biochemical results to computationally dock the photoaffinity ligands into DAT. Recent advancements in computational protein folding have legitimized its use in modeling integral membrane proteins. Docked structures consistent with current and ongoing biochemical data will be further refined using molecular dynamics. The results obtained from biochemical and computational analyses will lead to hypotheses that will be experimentally tested using site-directed mutagenesis, cysteine-scanning accessibility, and a library of DAT antagonist analogs to evaluate the molecular predictions. The integration of findings from these approaches will provide significant information related to the structure of the DAT active site and how antagonists exert effects on transport.
PUBLIC HEALTH RELEVANCE: The dopamine transporter is a major target of several drugs of abuse and has been linked to addiction and drug seeking behaviors, and as such is a highly clinically relevant protein. However, we still do not understand many of the details regarding the basis of drug recognition at DAT, or how various DAT drugs induce particular behavioral outcomes. Understanding the molecular basis of these properties could lead to important advances in clinical targeting of DAT as well as the related norepinephrine and serotonin transporters, all which play critical roles in our neurological and psychological health.
描述(由申请人提供):本项目将研究药物化合物如何利用结合生物化学和计算方法的综合方法与多巴胺转运蛋白(DAT)相互作用。生化分析将使用新型的光亲和性抑制剂,不可逆地连接到转运蛋白。这些研究将确定药物和DAT之间的接触点以及结合位点中配体的分子取向。与此同时,我们将进行计算研究,以建立DAT的比较模型的基础上同源性的竞争对手结合的晶体结构,从相关的细菌转运蛋白,LeuT。比较模型将被用于与生物化学的结果计算对接到DAT的光亲和配体。最近的进展,计算蛋白质折叠合法化它的使用在建模的完整的膜蛋白。与当前和正在进行的生化数据一致的对接结构将使用分子动力学进一步完善。从生物化学和计算分析获得的结果将导致的假设,将实验测试使用定点诱变,半胱氨酸扫描的可访问性,和一个数据库的DAT拮抗剂类似物,以评估分子预测。这些方法的结果的整合将提供有关DAT活性位点的结构以及拮抗剂如何对转运产生影响的重要信息。
公共卫生关系:多巴胺转运蛋白是几种滥用药物的主要靶点,与成瘾和药物寻求行为有关,因此是一种高度临床相关的蛋白质。然而,我们仍然不了解DAT中药物识别基础的许多细节,或者各种DAT药物如何诱导特定的行为结果。了解这些特性的分子基础可能会导致DAT以及相关的去甲肾上腺素和5-羟色胺转运蛋白的临床靶向的重要进展,所有这些都在我们的神经和心理健康中发挥着关键作用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
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Loren Keith Henry其他文献
Loren Keith Henry的其他文献
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{{ truncateString('Loren Keith Henry', 18)}}的其他基金
Computational and Biochemical Docking of Dopamine Transporter Antagonists
多巴胺转运蛋白拮抗剂的计算和生化对接
- 批准号:
8212147 - 财政年份:2010
- 资助金额:
$ 29.89万 - 项目类别:
Computational and Biochemical Docking of Dopamine Transporter Antagonists
多巴胺转运蛋白拮抗剂的计算和生化对接
- 批准号:
8415923 - 财政年份:2010
- 资助金额:
$ 29.89万 - 项目类别:
Computational and Biochemical Docking of Dopamine Transporter Antagonists
多巴胺转运蛋白拮抗剂的计算和生化对接
- 批准号:
8585841 - 财政年份:2010
- 资助金额:
$ 29.89万 - 项目类别:
Integration of Computational and Biological Analysis of Serotonin Transporters
血清素转运蛋白的计算和生物学分析的整合
- 批准号:
7657276 - 财政年份:2007
- 资助金额:
$ 29.89万 - 项目类别:
Integration of Computational and Biological Analysis of Serotonin Transporters
血清素转运蛋白的计算和生物学分析的整合
- 批准号:
7320103 - 财政年份:2007
- 资助金额:
$ 29.89万 - 项目类别:
Integration of Computational and Biological Analysis of Serotonin Transporters
血清素转运蛋白的计算和生物学分析的整合
- 批准号:
7588177 - 财政年份:2007
- 资助金额:
$ 29.89万 - 项目类别:
Integration of Computational and Biological Analysis of Serotonin Transporters
血清素转运蛋白的计算和生物学分析的整合
- 批准号:
7474576 - 财政年份:2007
- 资助金额:
$ 29.89万 - 项目类别:
Role of epigenetics on long-lasting behavioral and gene-expression changes following neonate exposure to antidepressants
表观遗传学对新生儿接触抗抑郁药后长期行为和基因表达变化的作用
- 批准号:
9795829 - 财政年份:
- 资助金额:
$ 29.89万 - 项目类别:
Role of epigenetics on long-lasting behavioral and gene-expression changes following neonate exposure to antidepressants
表观遗传学对新生儿接触抗抑郁药后长期行为和基因表达变化的作用
- 批准号:
9976550 - 财政年份:
- 资助金额:
$ 29.89万 - 项目类别:
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